Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Distinct behavior of connexin56 and connexin46 gap junctional channels can be predicted from the behavior of their hemi-gap-junctional channels.
Ebihara L
,
Berthoud VM
,
Beyer EC
.
???displayArticle.abstract???
The gap-junctional protein rat connexin46 (Cx46) has the unusual ability to form voltage-gated channels in the nonjunctional plasma membrane of Xenopus oocytes (Paul et al., 1991; Ebihara and Steiner, 1993). These have been suggested to be gap-junctional hemichannels or connexons. The Xenopus oocyte system was used to characterize the functional properties of a closely related lens gap-junctional protein, chicken connexin56 (Cx56) (Rup et al., 1993) and to contrast them to those of rat Cx46. Single oocytes injected with either Cx56 or Cx46 cRNA developed time-dependent, outward currents that activated on depolarization. The currents induced by Cx56 and Cx46 showed differences in steady-state voltage dependence and in their degree of rectification. Furthermore, the voltage-dependent properties of the nonjunctional channels induced by the connexin cRNAs in external solutions containing low concentrations of calcium ions could account remarkably well for the behavior of the intercellular channels formed by Cx56 and Cx46 in paired oocytes. These results suggest that many of the voltage-dependent properties of the hemi-gap-junctional channels are retained by the intercellular channels.
Barrio,
Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage.
1991, Pubmed,
Xenbase
Barrio,
Gap junctions formed by connexins 26 and 32 alone and in combination are differently affected by applied voltage.
1991,
Pubmed
,
Xenbase
Beyer,
Connexin43: a protein from rat heart homologous to a gap junction protein from liver.
1987,
Pubmed
Beyer,
Connexin family of gap junction proteins.
1990,
Pubmed
Bruzzone,
Connexin40, a component of gap junctions in vascular endothelium, is restricted in its ability to interact with other connexins.
1993,
Pubmed
,
Xenbase
Dahl,
Expression of functional cell-cell channels from cloned rat liver gap junction complementary DNA.
1987,
Pubmed
,
Xenbase
Ebihara,
Properties of a nonjunctional current expressed from a rat connexin46 cDNA in Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Goodenough,
Topological distribution of two connexin32 antigenic sites in intact and split rodent hepatocyte gap junctions.
1988,
Pubmed
Hennemann,
Characterization of gap junction genes expressed in F9 embryonic carcinoma cells: molecular cloning of mouse connexin31 and -45 cDNAs.
1992,
Pubmed
Hennemann,
Two gap junction genes, connexin 31.1 and 30.3, are closely linked on mouse chromosome 4 and preferentially expressed in skin.
1992,
Pubmed
,
Xenbase
Hennemann,
Molecular cloning and functional expression of mouse connexin40, a second gap junction gene preferentially expressed in lung.
1992,
Pubmed
,
Xenbase
Kanter,
Cardiac myocytes express multiple gap junction proteins.
1992,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Malchow,
A novel action of quinine and quinidine on the membrane conductance of neurons from the vertebrate retina.
1994,
Pubmed
Methfessel,
Patch clamp measurements on Xenopus laevis oocytes: currents through endogenous channels and implanted acetylcholine receptor and sodium channels.
1986,
Pubmed
,
Xenbase
Milks,
Topology of the 32-kd liver gap junction protein determined by site-directed antibody localizations.
1988,
Pubmed
Musil,
Biochemical analysis of connexin43 intracellular transport, phosphorylation, and assembly into gap junctional plaques.
1991,
Pubmed
Musil,
Multisubunit assembly of an integral plasma membrane channel protein, gap junction connexin43, occurs after exit from the ER.
1993,
Pubmed
,
Xenbase
Paul,
Molecular cloning of cDNA for rat liver gap junction protein.
1986,
Pubmed
Paul,
Connexin46, a novel lens gap junction protein, induces voltage-gated currents in nonjunctional plasma membrane of Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Rup,
Chick connexin-56, a novel lens gap junction protein. Molecular cloning and functional expression.
1993,
Pubmed
Spray,
Equilibrium properties of a voltage-dependent junctional conductance.
1981,
Pubmed
,
Xenbase
Suchyna,
Identification of a proline residue as a transduction element involved in voltage gating of gap junctions.
1993,
Pubmed
,
Xenbase
Swenson,
Formation of gap junctions by expression of connexins in Xenopus oocyte pairs.
1989,
Pubmed
,
Xenbase
Verselis,
Opposite voltage gating polarities of two closely related connexins.
1994,
Pubmed
,
Xenbase
White,
Voltage gating of connexins.
1994,
Pubmed
,
Xenbase
White,
Mouse Cx50, a functional member of the connexin family of gap junction proteins, is the lens fiber protein MP70.
1992,
Pubmed
,
Xenbase
White,
Selective interactions among the multiple connexin proteins expressed in the vertebrate lens: the second extracellular domain is a determinant of compatibility between connexins.
1994,
Pubmed
,
Xenbase
Willecke,
Mouse connexin37: cloning and functional expression of a gap junction gene highly expressed in lung.
1991,
Pubmed
,
Xenbase
Yancey,
The 43-kD polypeptide of heart gap junctions: immunolocalization, topology, and functional domains.
1989,
Pubmed
Zhang,
Sequence and tissue distribution of a second protein of hepatic gap junctions, Cx26, as deduced from its cDNA.
1989,
Pubmed
