Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The Nup107-160 nucleoporin complex is required for correct bipolar spindle assembly.
Orjalo AV
,
Arnaoutov A
,
Shen Z
,
Boyarchuk Y
,
Zeitlin SG
,
Fontoura B
,
Briggs S
,
Dasso M
,
Forbes DJ
.
???displayArticle.abstract???
The Nup107-160 complex is a critical subunit of the nuclear pore. This complex localizes to kinetochores in mitotic mammalian cells, where its function is unknown. To examine Nup107-160 complex recruitment to kinetochores, we stained human cells with antisera to four complex components. Each antibody stained not only kinetochores but also prometaphase spindle poles and proximalspindle fibers, mirroring the dual prometaphase localization of the spindle checkpoint proteins Mad1, Mad2, Bub3, and Cdc20. Indeed, expanded crescents of the Nup107-160 complex encircled unattached kinetochores, similar to the hyperaccumulation observed of dynamic outer kinetochore checkpoint proteins and motors at unattached kinetochores. In mitotic Xenopus egg extracts, the Nup107-160 complex localized throughout reconstituted spindles. When the Nup107-160 complex was depleted from extracts, the spindle checkpoint remained intact, but spindle assembly was rendered strikingly defective. Microtubule nucleation around sperm centrosomes seemed normal, but the microtubules quickly disassembled, leaving largely unattached sperm chromatin. Notably, Ran-GTP caused normal assembly of microtubule asters in depleted extracts, indicating that this defect was upstream of Ran or independent of it. We conclude that the Nup107-160 complex is dynamic in mitosis and that it promotes spindle assembly in a manner that is distinct from its functions at interphase nuclear pores.
Aitchison,
Nup120p: a yeast nucleoporin required for NPC distribution and mRNA transport.
1995, Pubmed
Aitchison,
Nup120p: a yeast nucleoporin required for NPC distribution and mRNA transport.
1995,
Pubmed
Arnaoutov,
The Ran GTPase regulates kinetochore function.
2003,
Pubmed
,
Xenbase
Arnaoutov,
Crm1 is a mitotic effector of Ran-GTP in somatic cells.
2005,
Pubmed
Askjaer,
Ran GTPase cycle and importins alpha and beta are essential for spindle formation and nuclear envelope assembly in living Caenorhabditis elegans embryos.
2002,
Pubmed
Baï,
The fission yeast Nup107-120 complex functionally interacts with the small GTPase Ran/Spi1 and is required for mRNA export, nuclear pore distribution, and proper cell division.
2004,
Pubmed
Bamba,
The GTPase Ran regulates chromosome positioning and nuclear envelope assembly in vivo.
2002,
Pubmed
,
Xenbase
Belgareh,
Functional characterization of a Nup159p-containing nuclear pore subcomplex.
1998,
Pubmed
Belgareh,
An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells.
2001,
Pubmed
Blower,
A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly.
2005,
Pubmed
,
Xenbase
Boehmer,
Depletion of a single nucleoporin, Nup107, prevents the assembly of a subset of nucleoporins into the nuclear pore complex.
2003,
Pubmed
Campbell,
Mitotic checkpoint proteins HsMAD1 and HsMAD2 are associated with nuclear pore complexes in interphase.
2001,
Pubmed
Carazo-Salas,
Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation.
1999,
Pubmed
,
Xenbase
Caudron,
Spatial coordination of spindle assembly by chromosome-mediated signaling gradients.
2005,
Pubmed
,
Xenbase
Clarke,
Cell biology. A gradient signal orchestrates the mitotic spindle.
2005,
Pubmed
,
Xenbase
Cronshaw,
Proteomic analysis of the mammalian nuclear pore complex.
2002,
Pubmed
Desai,
The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro.
1999,
Pubmed
,
Xenbase
Devos,
Components of coated vesicles and nuclear pore complexes share a common molecular architecture.
2004,
Pubmed
Di Fiore,
Mammalian RanBP1 regulates centrosome cohesion during mitosis.
2003,
Pubmed
,
Xenbase
Di Fiore,
Mitotic functions of the Ran GTPase network: the importance of being in the right place at the right time.
2004,
Pubmed
Doye,
A novel nuclear pore protein Nup133p with distinct roles in poly(A)+ RNA transport and nuclear pore distribution.
1994,
Pubmed
Ems-McClung,
Importin alpha/beta and Ran-GTP regulate XCTK2 microtubule binding through a bipartite nuclear localization signal.
2004,
Pubmed
,
Xenbase
Enninga,
Sec13 shuttles between the nucleus and the cytoplasm and stably interacts with Nup96 at the nuclear pore complex.
2003,
Pubmed
Fontoura,
A conserved biogenesis pathway for nucleoporins: proteolytic processing of a 186-kilodalton precursor generates Nup98 and the novel nucleoporin, Nup96.
1999,
Pubmed
Gadde,
Mechanisms and molecules of the mitotic spindle.
2004,
Pubmed
Goldstein,
Pleiotropic nuclear defects associated with a conditional allele of the novel nucleoporin Rat9p/Nup85p.
1996,
Pubmed
Gruss,
Ran induces spindle assembly by reversing the inhibitory effect of importin alpha on TPX2 activity.
2001,
Pubmed
,
Xenbase
Harel,
Importin beta negatively regulates nuclear membrane fusion and nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase
Harel,
Importin beta: conducting a much larger cellular symphony.
2004,
Pubmed
Harel,
Removal of a single pore subcomplex results in vertebrate nuclei devoid of nuclear pores.
2003,
Pubmed
,
Xenbase
Heald,
Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts.
1996,
Pubmed
,
Xenbase
Heath,
Nuclear pore complex clustering and nuclear accumulation of poly(A)+ RNA associated with mutation of the Saccharomyces cerevisiae RAT2/NUP120 gene.
1995,
Pubmed
Hetzer,
GTP hydrolysis by Ran is required for nuclear envelope assembly.
2000,
Pubmed
,
Xenbase
Hetzer,
The Ran GTPase as a marker of chromosome position in spindle formation and nuclear envelope assembly.
2002,
Pubmed
Hetzer,
Pushing the envelope: structure, function, and dynamics of the nuclear periphery.
2005,
Pubmed
Hoffman,
Microtubule-dependent changes in assembly of microtubule motor proteins and mitotic spindle checkpoint proteins at PtK1 kinetochores.
2001,
Pubmed
Howell,
Spindle checkpoint protein dynamics at kinetochores in living cells.
2004,
Pubmed
Iouk,
The yeast nuclear pore complex functionally interacts with components of the spindle assembly checkpoint.
2002,
Pubmed
Jeganathan,
The Rae1-Nup98 complex prevents aneuploidy by inhibiting securin degradation.
2005,
Pubmed
Joseph,
SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles.
2002,
Pubmed
Joseph,
The RanGAP1-RanBP2 complex is essential for microtubule-kinetochore interactions in vivo.
2004,
Pubmed
Kalab,
The ran GTPase regulates mitotic spindle assembly.
1999,
Pubmed
,
Xenbase
Kalab,
Visualization of a Ran-GTP gradient in interphase and mitotic Xenopus egg extracts.
2002,
Pubmed
,
Xenbase
Karsenti,
The mitotic spindle: a self-made machine.
2001,
Pubmed
,
Xenbase
Kastenmayer,
The C-terminal half of Saccharomyces cerevisiae Mad1p mediates spindle checkpoint function, chromosome transmission fidelity and CEN association.
2005,
Pubmed
Keryer,
Part of Ran is associated with AKAP450 at the centrosome: involvement in microtubule-organizing activity.
2003,
Pubmed
,
Xenbase
Kraemer,
Mrnp41 (Rae 1p) associates with microtubules in HeLa cells and in neurons.
2001,
Pubmed
Kutay,
Dominant-negative mutants of importin-beta block multiple pathways of import and export through the nuclear pore complex.
1997,
Pubmed
,
Xenbase
Li,
Phosphorylation of RCC1 in mitosis is essential for producing a high RanGTP concentration on chromosomes and for spindle assembly in mammalian cells.
2004,
Pubmed
,
Xenbase
Li,
Mutation or deletion of the Saccharomyces cerevisiae RAT3/NUP133 gene causes temperature-dependent nuclear accumulation of poly(A)+ RNA and constitutive clustering of nuclear pore complexes.
1995,
Pubmed
Liu,
Human MPS1 kinase is required for mitotic arrest induced by the loss of CENP-E from kinetochores.
2003,
Pubmed
Loïodice,
The entire Nup107-160 complex, including three new members, is targeted as one entity to kinetochores in mitosis.
2004,
Pubmed
Lutzmann,
Modular self-assembly of a Y-shaped multiprotein complex from seven nucleoporins.
2002,
Pubmed
Macara,
Transport into and out of the nucleus.
2001,
Pubmed
Maiato,
The dynamic kinetochore-microtubule interface.
2004,
Pubmed
Merdes,
A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly.
1996,
Pubmed
,
Xenbase
Minshull,
A MAP kinase-dependent spindle assembly checkpoint in Xenopus egg extracts.
1994,
Pubmed
,
Xenbase
Mitchison,
Bipolarization and poleward flux correlate during Xenopus extract spindle assembly.
2004,
Pubmed
,
Xenbase
Mitchison,
Mechanism and function of poleward flux in Xenopus extract meiotic spindles.
2005,
Pubmed
,
Xenbase
Murray,
Cell cycle extracts.
1991,
Pubmed
Nachury,
Importin beta is a mitotic target of the small GTPase Ran in spindle assembly.
2001,
Pubmed
,
Xenbase
Ohba,
Self-organization of microtubule asters induced in Xenopus egg extracts by GTP-bound Ran.
1999,
Pubmed
,
Xenbase
Pemberton,
Disruption of the nucleoporin gene NUP133 results in clustering of nuclear pore complexes.
1995,
Pubmed
Quimby,
Ran GTPase regulates Mad2 localization to the nuclear pore complex.
2005,
Pubmed
Salina,
Nup358 integrates nuclear envelope breakdown with kinetochore assembly.
2003,
Pubmed
Sampath,
The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly.
2004,
Pubmed
,
Xenbase
Shah,
Major binding sites for the nuclear import receptor are the internal nucleoporin Nup153 and the adjacent nuclear filament protein Tpr.
1998,
Pubmed
,
Xenbase
Shannon,
Mad2 and BubR1 function in a single checkpoint pathway that responds to a loss of tension.
2002,
Pubmed
Siniossoglou,
A novel complex of nucleoporins, which includes Sec13p and a Sec13p homolog, is essential for normal nuclear pores.
1996,
Pubmed
Siniossoglou,
Structure and assembly of the Nup84p complex.
2000,
Pubmed
Stukenberg,
The kinetochore NUPtials.
2003,
Pubmed
Suntharalingam,
Peering through the pore: nuclear pore complex structure, assembly, and function.
2003,
Pubmed
Taylor,
The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase.
1998,
Pubmed
Teixeira,
Two functionally distinct domains generated by in vivo cleavage of Nup145p: a novel biogenesis pathway for nucleoporins.
1997,
Pubmed
Trieselmann,
Ran localizes around the microtubule spindle in vivo during mitosis in Drosophila embryos.
2002,
Pubmed
Trieselmann,
Ran modulates spindle assembly by regulating a subset of TPX2 and Kid activities including Aurora A activation.
2003,
Pubmed
,
Xenbase
Tsai,
A Ran signalling pathway mediated by the mitotic kinase Aurora A in spindle assembly.
2003,
Pubmed
,
Xenbase
Vasu,
Novel vertebrate nucleoporins Nup133 and Nup160 play a role in mRNA export.
2001,
Pubmed
,
Xenbase
Vasu,
Nuclear pores and nuclear assembly.
2001,
Pubmed
Walther,
The conserved Nup107-160 complex is critical for nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase
Walther,
RanGTP mediates nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase
Whalen,
Advancement through mitosis requires rae1 gene function in fission yeast.
1997,
Pubmed
Wiese,
Role of importin-beta in coupling Ran to downstream targets in microtubule assembly.
2001,
Pubmed
,
Xenbase
Wilde,
Stimulation of microtubule aster formation and spindle assembly by the small GTPase Ran.
1999,
Pubmed
,
Xenbase
Zhang,
Roles of Ran-GTP and Ran-GDP in precursor vesicle recruitment and fusion during nuclear envelope assembly in a human cell-free system.
2001,
Pubmed
,
Xenbase
Zhang,
Ran-GTP stabilises microtubule asters and inhibits nuclear assembly in Xenopus egg extracts.
1999,
Pubmed
,
Xenbase
Zhang,
Chromatin-independent nuclear envelope assembly induced by Ran GTPase in Xenopus egg extracts.
2000,
Pubmed
,
Xenbase
