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Mol Biol Cell
2003 Jun 01;146:2262-76. doi: 10.1091/mbc.e02-07-0377.
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RHAMM is a centrosomal protein that interacts with dynein and maintains spindle pole stability.
Maxwell CA
,
Keats JJ
,
Crainie M
,
Sun X
,
Yen T
,
Shibuya E
,
Hendzel M
,
Chan G
,
Pilarski LM
.
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The receptor for hyaluronan-mediated motility (RHAMM), an acidic coiled coil protein, has previously been characterized as a cell surface receptor for hyaluronan, and a microtubule-associated intracellular hyaluronan binding protein. In this study, we demonstrate that a subset of cellular RHAMM localizes to the centrosome and functions in the maintenance of spindle integrity. We confirm a previous study showing that the amino terminus of RHAMM interacts with microtubules and further demonstrate that a separate carboxy-terminal domain is required for centrosomal targeting. This motif overlaps the defined hyaluronan binding domain and bears 72% identity to the dynein interaction domain of Xklp2. RHAMM antibodies coimmunprecipitate dynein IC from Xenopus and HeLa extracts. Deregulation of RHAMM expression inhibits mitotic progression and affects spindle architecture. Structure, localization, and function, along with phylogenetic analysis, suggests that RHAMM may be a new member of the TACC family. Thus, we demonstrate a novel centrosomal localization and mitotic spindle-stabilizing function for RHAMM. Moreover, we provide a potential mechanism for this function in that RHAMM may cross-link centrosomal microtubules, through a direct interaction with microtubules and an association with dynein.
Askham,
Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome.
2002, Pubmed
Askham,
Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome.
2002,
Pubmed
Assmann,
The intracellular hyaluronan receptor RHAMM/IHABP interacts with microtubules and actin filaments.
1999,
Pubmed
Bhattacharya,
Pim-1 associates with protein complexes necessary for mitosis.
2002,
Pubmed
Boleti,
Xklp2, a novel Xenopus centrosomal kinesin-like protein required for centrosome separation during mitosis.
1996,
Pubmed
,
Xenbase
Briggman,
NuMA: evaluation of a new biomarker for the detection of low stage colorectal cancer.
1999,
Pubmed
Brocchieri,
Phylogenetic inferences from molecular sequences: review and critique.
2001,
Pubmed
Chen,
AZU-1: a candidate breast tumor suppressor and biomarker for tumor progression.
2000,
Pubmed
Cokol,
Finding nuclear localization signals.
2000,
Pubmed
Compton,
Identification of novel centromere/kinetochore-associated proteins using monoclonal antibodies generated against human mitotic chromosome scaffolds.
1991,
Pubmed
Crainie,
Overexpression of the receptor for hyaluronan-mediated motility (RHAMM) characterizes the malignant clone in multiple myeloma: identification of three distinct RHAMM variants.
1999,
Pubmed
Cullen,
Msps protein is localized to acentrosomal poles to ensure bipolarity of Drosophila meiotic spindles.
2001,
Pubmed
Dionne,
ch-TOGp is required for microtubule aster formation in a mammalian mitotic extract.
2000,
Pubmed
Doxsey,
Pericentrin, a highly conserved centrosome protein involved in microtubule organization.
1994,
Pubmed
,
Xenbase
Gaglio,
Opposing motor activities are required for the organization of the mammalian mitotic spindle pole.
1996,
Pubmed
,
Xenbase
Gares,
During human thymic development, beta 1 integrins regulate adhesion, motility, and the outcome of RHAMM/hyaluronan engagement.
1998,
Pubmed
Gavanescu,
Centrosome proteins: a major class of autoantigens in scleroderma.
1999,
Pubmed
Gergely,
D-TACC: a novel centrosomal protein required for normal spindle function in the early Drosophila embryo.
2000,
Pubmed
Gergely,
The TACC domain identifies a family of centrosomal proteins that can interact with microtubules.
2000,
Pubmed
Giet,
Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules.
2002,
Pubmed
,
Xenbase
Gillingham,
The PACT domain, a conserved centrosomal targeting motif in the coiled-coil proteins AKAP450 and pericentrin.
2000,
Pubmed
Gordon,
Chromosome movement in mitosis requires microtubule anchorage at spindle poles.
2001,
Pubmed
Greiner,
Receptor for hyaluronan acid-mediated motility (RHAMM) is a new immunogenic leukemia-associated antigen in acute and chronic myeloid leukemia.
2002,
Pubmed
Gruss,
Chromosome-induced microtubule assembly mediated by TPX2 is required for spindle formation in HeLa cells.
2002,
Pubmed
,
Xenbase
Hall,
Overexpression of the hyaluronan receptor RHAMM is transforming and is also required for H-ras transformation.
1995,
Pubmed
Hardwick,
Molecular cloning of a novel hyaluronan receptor that mediates tumor cell motility.
1992,
Pubmed
Haren,
Direct binding of NuMA to tubulin is mediated by a novel sequence motif in the tail domain that bundles and stabilizes microtubules.
2002,
Pubmed
,
Xenbase
Kufer,
Human TPX2 is required for targeting Aurora-A kinase to the spindle.
2002,
Pubmed
,
Xenbase
Lee,
Msps/XMAP215 interacts with the centrosomal protein D-TACC to regulate microtubule behaviour.
2001,
Pubmed
,
Xenbase
Line,
Characterisation of tumour-associated antigens in colon cancer.
2002,
Pubmed
Lynn,
Identification of sequence, protein isoforms, and distribution of the hyaluronan-binding protein RHAMM in adult and developing rat brain.
2001,
Pubmed
Masellis-Smith,
Hyaluronan-dependent motility of B cells and leukemic plasma cells in blood, but not of bone marrow plasma cells, in multiple myeloma: alternate use of receptor for hyaluronan-mediated motility (RHAMM) and CD44.
1996,
Pubmed
Merdes,
Formation of spindle poles by dynein/dynactin-dependent transport of NuMA.
2000,
Pubmed
Merdes,
A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly.
1996,
Pubmed
,
Xenbase
Novatchkova,
A CH domain-containing N terminus in NuMA?
2002,
Pubmed
Piekorz,
The centrosomal protein TACC3 is essential for hematopoietic stem cell function and genetically interfaces with p53-regulated apoptosis.
2002,
Pubmed
Purohit,
Direct interaction of pericentrin with cytoplasmic dynein light intermediate chain contributes to mitotic spindle organization.
1999,
Pubmed
Rattner,
Autoantibodies to components of the mitotic apparatus.
1998,
Pubmed
Spicer,
The human and mouse receptors for hyaluronan-mediated motility, RHAMM, genes (HMMR) map to human chromosome 5q33.2-qter and mouse chromosome 11.
1995,
Pubmed
Steadman,
Transforming acidic coiled-coil-containing protein 4 interacts with centrosomal AKAP350 and the mitotic spindle apparatus.
2002,
Pubmed
Still,
The third member of the transforming acidic coiled coil-containing gene family, TACC3, maps in 4p16, close to translocation breakpoints in multiple myeloma, and is upregulated in various cancer cell lines.
1999,
Pubmed
Still,
Cloning of TACC1, an embryonically expressed, potentially transforming coiled coil containing gene, from the 8p11 breast cancer amplicon.
1999,
Pubmed
Tatusova,
BLAST 2 Sequences, a new tool for comparing protein and nucleotide sequences.
1999,
Pubmed
Turley,
Hyaluronan and cell locomotion.
1992,
Pubmed
Turley,
Localization of hyaluronate and hyaluronate-binding protein on motile and non-motile fibroblasts.
1985,
Pubmed
Turley,
Hyaluronan and a cell-associated hyaluronan binding protein regulate the locomotion of ras-transformed cells.
1991,
Pubmed
Turley,
Characterization of hyaluronate binding proteins isolated from 3T3 and murine sarcoma virus transformed 3T3 cells.
1987,
Pubmed
Wang,
The overexpression of RHAMM, a hyaluronan-binding protein that regulates ras signaling, correlates with overexpression of mitogen-activated protein kinase and is a significant parameter in breast cancer progression.
1998,
Pubmed
Wishart,
A platform-independent graphical user interface for SEQSEE and XALIGN.
1997,
Pubmed
Wittmann,
Localization of the kinesin-like protein Xklp2 to spindle poles requires a leucine zipper, a microtubule-associated protein, and dynein.
1998,
Pubmed
,
Xenbase
Yang,
Identification of two hyaluronan-binding domains in the hyaluronan receptor RHAMM.
1993,
Pubmed
Yang,
Identification of a common hyaluronan binding motif in the hyaluronan binding proteins RHAMM, CD44 and link protein.
1994,
Pubmed
Young,
Cytoplasmic dynein-mediated assembly of pericentrin and gamma tubulin onto centrosomes.
2000,
Pubmed
,
Xenbase
Zeng,
NuMA: a nuclear protein involved in mitotic centrosome function.
2000,
Pubmed
,
Xenbase
Zimmerman,
Construction of centrosomes and spindle poles by molecular motor-driven assembly of protein particles.
2000,
Pubmed
