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Chromatin-induced spindle assembly depends on regulation of microtubule-depolymerizing proteins by the chromosomal passenger complex (CPC), consisting of Incenp, Survivin, Dasra (Borealin), and the kinase Aurora B, but the mechanism and significance of the spatial regulation of Aurora B activity remain unclear. Here, we show that the Aurora B pathway is suppressed in the cytoplasm of Xenopus egg extract by phosphatases, but that it becomes activated by chromatin via a Ran-independent mechanism. While spindle microtubule assembly normally requires Dasra-dependent chromatin binding of the CPC, this function of Dasra can be bypassed by clustering Aurora B-Incenp by using anti-Incenp antibodies, which stimulate autoactivation among bound complexes. However, such chromatin-independent Aurora B pathway activation promotes centrosomal microtubule assembly and produces aberrant achromosomal spindle-like structures. We propose that chromosomal enrichment of the CPC results in local kinase autoactivation, a mechanism that contributes to the spatial regulation of spindle assembly and possibly to other mitotic processes.
Andersen,
Mitotic chromatin regulates phosphorylation of Stathmin/Op18.
1997, Pubmed,
Xenbase
Andersen,
Mitotic chromatin regulates phosphorylation of Stathmin/Op18.
1997,
Pubmed
,
Xenbase
Andrews,
Aurora B regulates MCAK at the mitotic centromere.
2004,
Pubmed
Bastiaens,
Gradients in the self-organization of the mitotic spindle.
2006,
Pubmed
,
Xenbase
Bishop,
Phosphorylation of the carboxyl terminus of inner centromere protein (INCENP) by the Aurora B Kinase stimulates Aurora B kinase activity.
2002,
Pubmed
Blower,
A Rae1-containing ribonucleoprotein complex is required for mitotic spindle assembly.
2005,
Pubmed
,
Xenbase
Bolton,
Aurora B kinase exists in a complex with survivin and INCENP and its kinase activity is stimulated by survivin binding and phosphorylation.
2002,
Pubmed
,
Xenbase
Budde,
Regulation of Op18 during spindle assembly in Xenopus egg extracts.
2001,
Pubmed
,
Xenbase
Carazo-Salas,
Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation.
1999,
Pubmed
,
Xenbase
Carazo-Salas,
Long-range communication between chromatin and microtubules in Xenopus egg extracts.
2003,
Pubmed
,
Xenbase
Carvalho,
Survivin is required for stable checkpoint activation in taxol-treated HeLa cells.
2003,
Pubmed
Cassimeris,
The oncoprotein 18/stathmin family of microtubule destabilizers.
2002,
Pubmed
Caudron,
Spatial coordination of spindle assembly by chromosome-mediated signaling gradients.
2005,
Pubmed
,
Xenbase
Chang,
Analysis of protein phosphorylation by hypothesis-driven multiple-stage mass spectrometry.
2004,
Pubmed
Desai,
Kin I kinesins are microtubule-destabilizing enzymes.
1999,
Pubmed
,
Xenbase
Desai,
The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro.
1999,
Pubmed
,
Xenbase
Félix,
Cdc2 H1 kinase is negatively regulated by a type 2A phosphatase in the Xenopus early embryonic cell cycle: evidence from the effects of okadaic acid.
1990,
Pubmed
,
Xenbase
Ferrari,
Aurora-A site specificity: a study with synthetic peptide substrates.
2005,
Pubmed
Funabiki,
The Xenopus chromokinesin Xkid is essential for metaphase chromosome alignment and must be degraded to allow anaphase chromosome movement.
2000,
Pubmed
,
Xenbase
Gadea,
Aurora kinase inhibitor ZM447439 blocks chromosome-induced spindle assembly, the completion of chromosome condensation, and the establishment of the spindle integrity checkpoint in Xenopus egg extracts.
2005,
Pubmed
,
Xenbase
Gadea,
Aurora B is required for mitotic chromatin-induced phosphorylation of Op18/Stathmin.
2006,
Pubmed
,
Xenbase
Gassmann,
Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle.
2004,
Pubmed
Goetz,
Membrane localization, oligomerization, and phosphorylation are required for optimal raf activation.
2003,
Pubmed
Gruss,
Ran induces spindle assembly by reversing the inhibitory effect of importin alpha on TPX2 activity.
2001,
Pubmed
,
Xenbase
Harel,
Importin beta: conducting a much larger cellular symphony.
2004,
Pubmed
Honda,
Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis.
2003,
Pubmed
Hunter,
The kinesin-related protein MCAK is a microtubule depolymerase that forms an ATP-hydrolyzing complex at microtubule ends.
2003,
Pubmed
Jin,
Quantitative analysis of protein phosphorylation in mouse brain by hypothesis-driven multistage mass spectrometry.
2005,
Pubmed
Kaláb,
Analysis of a RanGTP-regulated gradient in mitotic somatic cells.
2006,
Pubmed
,
Xenbase
Kang,
Functional cooperation of Dam1, Ipl1, and the inner centromere protein (INCENP)-related protein Sli15 during chromosome segregation.
2001,
Pubmed
Kholodenko,
Diffusion control of protein phosphorylation in signal transduction pathways.
2000,
Pubmed
Knowlton,
Aurora B is enriched at merotelic attachment sites, where it regulates MCAK.
2006,
Pubmed
,
Xenbase
Küntziger,
Stathmin/Op18 phosphorylation is regulated by microtubule assembly.
2001,
Pubmed
,
Xenbase
Lan,
Aurora B phosphorylates centromeric MCAK and regulates its localization and microtubule depolymerization activity.
2004,
Pubmed
,
Xenbase
Lens,
Survivin is required for a sustained spindle checkpoint arrest in response to lack of tension.
2003,
Pubmed
Murnion,
Chromatin-associated protein phosphatase 1 regulates aurora-B and histone H3 phosphorylation.
2001,
Pubmed
,
Xenbase
Murray,
Cyclin synthesis drives the early embryonic cell cycle.
1989,
Pubmed
,
Xenbase
Murray,
Cell cycle extracts.
1991,
Pubmed
Nachury,
Importin beta is a mitotic target of the small GTPase Ran in spindle assembly.
2001,
Pubmed
,
Xenbase
Nemergut,
Chromatin docking and exchange activity enhancement of RCC1 by histones H2A and H2B.
2001,
Pubmed
,
Xenbase
Niethammer,
Stathmin-tubulin interaction gradients in motile and mitotic cells.
2004,
Pubmed
,
Xenbase
Ohi,
Differentiation of cytoplasmic and meiotic spindle assembly MCAK functions by Aurora B-dependent phosphorylation.
2004,
Pubmed
,
Xenbase
Ribbeck,
NuSAP, a mitotic RanGTP target that stabilizes and cross-links microtubules.
2006,
Pubmed
,
Xenbase
Romano,
CSC-1: a subunit of the Aurora B kinase complex that binds to the survivin-like protein BIR-1 and the incenp-like protein ICP-1.
2003,
Pubmed
Sampath,
The chromosomal passenger complex is required for chromatin-induced microtubule stabilization and spindle assembly.
2004,
Pubmed
,
Xenbase
Sawin,
Mitotic spindle assembly by two different pathways in vitro.
1991,
Pubmed
,
Xenbase
Sessa,
Mechanism of Aurora B activation by INCENP and inhibition by hesperadin.
2005,
Pubmed
,
Xenbase
Song,
Molecular cloning and characterization of a novel inhibitor of apoptosis protein from Xenopus laevis.
2003,
Pubmed
,
Xenbase
Tsai,
Aurora A kinase-coated beads function as microtubule-organizing centers and enhance RanGTP-induced spindle assembly.
2005,
Pubmed
,
Xenbase
Vader,
Survivin mediates targeting of the chromosomal passenger complex to the centromere and midbody.
2006,
Pubmed
Vagnarelli,
Chromosomal passengers: the four-dimensional regulation of mitotic events.
2004,
Pubmed
Wheatley,
INCENP binds directly to tubulin and requires dynamic microtubules to target to the cleavage furrow.
2001,
Pubmed
Wiese,
Role of importin-beta in coupling Ran to downstream targets in microtubule assembly.
2001,
Pubmed
,
Xenbase
Yasui,
Autophosphorylation of a newly identified site of Aurora-B is indispensable for cytokinesis.
2004,
Pubmed
Zhang,
An allosteric mechanism for activation of the kinase domain of epidermal growth factor receptor.
2006,
Pubmed
