Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Structure
2012 Oct 10;2010:1629-40. doi: 10.1016/j.str.2012.08.022.
Show Gene links
Show Anatomy links
Nano-positioning system for structural analysis of functional homomeric proteins in multiple conformations.
Hyde HC
,
Sandtner W
,
Vargas E
,
Dagcan AT
,
Robertson JL
,
Roux B
,
Correa AM
,
Bezanilla F
.
???displayArticle.abstract???
Proteins may undergo multiple conformational changes required for their function. One strategy used to estimate target-site positions in unknown structural conformations involves single-pair resonance energy transfer (RET) distance measurements. However, interpretation of inter-residue distances is difficult when applied to three-dimensional structural rearrangements, especially in homomeric systems. We developed a positioning method using inverse trilateration/triangulation to map target sites within a homomeric protein in all defined states, with simultaneous functional recordings. The procedure accounts for probe diffusion to accurately determine the three-dimensional position and confidence region of lanthanide LRET donors attached to a target site (one per subunit), relative to a single fluorescent acceptor placed in a static site. As first application, the method is used to determine the position of a functional voltage-gated potassium channel's voltage sensor. Our results verify the crystal structure relaxed conformation and report on the resting and active conformations for which crystal structures are not available.
Barthelmes,
Engineering encodable lanthanide-binding tags into loop regions of proteins.
2011, Pubmed
Barthelmes,
Engineering encodable lanthanide-binding tags into loop regions of proteins.
2011,
Pubmed
Bezanilla,
How membrane proteins sense voltage.
2008,
Pubmed
Campos,
Two atomic constraints unambiguously position the S4 segment relative to S1 and S2 segments in the closed state of Shaker K channel.
2007,
Pubmed
,
Xenbase
Cha,
Atomic scale movement of the voltage-sensing region in a potassium channel measured via spectroscopy.
1999,
Pubmed
Chen,
Structure of the full-length Shaker potassium channel Kv1.2 by normal-mode-based X-ray crystallographic refinement.
2010,
Pubmed
Eriksson,
Modeling the structure of agitoxin in complex with the Shaker K+ channel: a computational approach based on experimental distance restraints extracted from thermodynamic mutant cycles.
2002,
Pubmed
Franz,
Lanthanide-binding tags as versatile protein coexpression probes.
2003,
Pubmed
Garcia,
Purification and characterization of three inhibitors of voltage-dependent K+ channels from Leiurus quinquestriatus var. hebraeus venom.
1994,
Pubmed
,
Xenbase
Goldstein,
A point mutation in a Shaker K+ channel changes its charybdotoxin binding site from low to high affinity.
1992,
Pubmed
,
Xenbase
Goldstein,
The charybdotoxin receptor of a Shaker K+ channel: peptide and channel residues mediating molecular recognition.
1994,
Pubmed
,
Xenbase
Gonzalez,
S3b amino acid residues do not shuttle across the bilayer in voltage-dependent Shaker K+ channels.
2005,
Pubmed
,
Xenbase
Gryczynski,
Resolution of end-to-end distance distributions of flexible molecules using quenching-induced variations of the Forster distance for fluorescence energy transfer.
1988,
Pubmed
Henrion,
Tracking a complete voltage-sensor cycle with metal-ion bridges.
2012,
Pubmed
,
Xenbase
Heyduk,
Luminescence energy transfer with lanthanide chelates: interpretation of sensitized acceptor decay amplitudes.
2001,
Pubmed
Hoshi,
Biophysical and molecular mechanisms of Shaker potassium channel inactivation.
1990,
Pubmed
,
Xenbase
Jensen,
Mechanism of voltage gating in potassium channels.
2012,
Pubmed
Jogini,
Dynamics of the Kv1.2 voltage-gated K+ channel in a membrane environment.
2007,
Pubmed
Lacroix,
Properties of deactivation gating currents in Shaker channels.
2011,
Pubmed
Lainé,
Atomic proximity between S4 segment and pore domain in Shaker potassium channels.
2003,
Pubmed
,
Xenbase
Lewis,
Atomic constraints between the voltage sensor and the pore domain in a voltage-gated K+ channel of known structure.
2008,
Pubmed
,
Xenbase
Long,
Voltage sensor of Kv1.2: structural basis of electromechanical coupling.
2005,
Pubmed
Muschielok,
Application of the nano-positioning system to the analysis of fluorescence resonance energy transfer networks.
2011,
Pubmed
Muschielok,
A nano-positioning system for macromolecular structural analysis.
2008,
Pubmed
Nitz,
Structural origin of the high affinity of a chemically evolved lanthanide-binding peptide.
2004,
Pubmed
Oliva,
Slow inactivation in voltage gated potassium channels is insensitive to the binding of pore occluding peptide toxins.
2005,
Pubmed
,
Xenbase
Pathak,
Closing in on the resting state of the Shaker K(+) channel.
2007,
Pubmed
Pettersen,
UCSF Chimera--a visualization system for exploratory research and analysis.
2004,
Pubmed
Posson,
Small vertical movement of a K+ channel voltage sensor measured with luminescence energy transfer.
2005,
Pubmed
,
Xenbase
Posson,
Extent of voltage sensor movement during gating of shaker K+ channels.
2008,
Pubmed
,
Xenbase
Richardson,
Distance measurements reveal a common topology of prokaryotic voltage-gated ion channels in the lipid bilayer.
2006,
Pubmed
Sali,
Comparative protein modelling by satisfaction of spatial restraints.
1993,
Pubmed
Sandtner,
In vivo measurement of intramolecular distances using genetically encoded reporters.
2007,
Pubmed
,
Xenbase
Sandtner,
Ouabain binding site in a functioning Na+/K+ ATPase.
2011,
Pubmed
,
Xenbase
Schow,
Coupling between the voltage-sensing and pore domains in a voltage-gated potassium channel.
2012,
Pubmed
Selvin,
Principles and biophysical applications of lanthanide-based probes.
2002,
Pubmed
Shimony,
Engineering a uniquely reactive thiol into a cysteine-rich peptide.
1994,
Pubmed
Sindbert,
Accurate distance determination of nucleic acids via Förster resonance energy transfer: implications of dye linker length and rigidity.
2011,
Pubmed
Tao,
A gating charge transfer center in voltage sensors.
2010,
Pubmed
,
Xenbase
Villalba-Galea,
S4-based voltage sensors have three major conformations.
2008,
Pubmed
Yarov-Yarovoy,
Structural basis for gating charge movement in the voltage sensor of a sodium channel.
2012,
Pubmed
