Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1997 Oct 01;1710:6076-86. doi: 10.1128/MCB.17.10.6076.
Show Gene links
Show Anatomy links
Interference with the expression of a novel human polycomb protein, hPc2, results in cellular transformation and apoptosis.
Satijn DP
,
Olson DJ
,
van der Vlag J
,
Hamer KM
,
Lambrechts C
,
Masselink H
,
Gunster MJ
,
Sewalt RG
,
van Driel R
,
Otte AP
.
???displayArticle.abstract???
Polycomb (Pc) is involved in the stable and heritable repression of homeotic gene activity during Drosophila development. Here, we report the identification of a novel human Pc homolog, hPc2. This gene is more closely related to a Xenopus Pc homolog, XPc, than to a previously described human Pc homolog, CBX2 (hPc1). However, the hPc2 and CBX2/hPc1 proteins colocalize in interphase nuclei of human U-2 OS osteosarcoma cells, suggesting that the proteins are part of a common protein complex. To study the functions of the novel human Pc homolog, we generated a mutant protein, delta hPc2, which lacks an evolutionarily conserved C-terminal domain. This C-terminal domain is important for hPc2 function, since the delta hPc2 mutant protein which lacks the C-terminal domain is unable to repress gene activity. Expression of the delta hPc2 protein, but not of the wild-type hPc2 protein, results in cellular transformation of mammalian cell lines as judged by phenotypic changes, altered marker gene expression, and anchorage-independent growth. Specifically in delta hPc2-transformed cells, the expression of the c-myc proto-oncogene is strongly enhanced and serum deprivation results in apoptosis. In contrast, overexpression of the wild-type hPc2 protein results in decreased c-myc expression. Our data suggest that hPc2 is a repressor of proto-oncogene activity and that interference with hPc2 function can lead to derepression of proto-oncogene transcription and subsequently to cellular transformation.
Alkema,
Identification of Bmi1-interacting proteins as constituents of a multimeric mammalian polycomb complex.
1997, Pubmed
Alkema,
Identification of Bmi1-interacting proteins as constituents of a multimeric mammalian polycomb complex.
1997,
Pubmed
Bradley,
A soluble form of Wnt-1 protein with mitogenic activity on mammary epithelial cells.
1995,
Pubmed
Brown,
A retrovirus vector expressing the putative mammary oncogene int-1 causes partial transformation of a mammary epithelial cell line.
1986,
Pubmed
Bunker,
Transcriptional repression by Drosophila and mammalian Polycomb group proteins in transfected mammalian cells.
1994,
Pubmed
Cohen,
Transformation by the Bmi-1 oncoprotein correlates with its subnuclear localization but not its transcriptional suppression activity.
1996,
Pubmed
Coré,
Altered cellular proliferation and mesoderm patterning in Polycomb-M33-deficient mice.
1997,
Pubmed
Evan,
Integrated control of cell proliferation and cell death by the c-myc oncogene.
1994,
Pubmed
Evan,
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
1985,
Pubmed
,
Xenbase
Evan,
Induction of apoptosis in fibroblasts by c-myc protein.
1992,
Pubmed
Fanidi,
Cooperative interaction between c-myc and bcl-2 proto-oncogenes.
1992,
Pubmed
Franke,
Polycomb and polyhomeotic are constituents of a multimeric protein complex in chromatin of Drosophila melanogaster.
1992,
Pubmed
Franke,
Mapping functional domains of the polycomb protein of Drosophila melanogaster.
1995,
Pubmed
Gavin,
Differential regulation of the Wnt gene family during pregnancy and lactation suggests a role in postnatal development of the mammary gland.
1992,
Pubmed
Gecz,
Assignment of a Polycomb-like chromobox gene (CBX2) to human chromosome 17q25.
1995,
Pubmed
Gunster,
Identification and characterization of interactions between the vertebrate polycomb-group protein BMI1 and human homologs of polyhomeotic.
1997,
Pubmed
Isfort,
Analysis of oncogenes, tumor suppressor genes, autocrine growth-factor production, and differentiation state of human osteosarcoma cell lines.
1995,
Pubmed
Kanno,
mel-18, a Polycomb group-related mammalian gene, encodes a transcriptional negative regulator with tumor suppressive activity.
1995,
Pubmed
Kingston,
Repression and activation by multiprotein complexes that alter chromatin structure.
1996,
Pubmed
Koopman,
Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis.
1994,
Pubmed
Lewis,
A gene complex controlling segmentation in Drosophila.
1978,
Pubmed
Martin,
Early redistribution of plasma membrane phosphatidylserine is a general feature of apoptosis regardless of the initiating stimulus: inhibition by overexpression of Bcl-2 and Abl.
1995,
Pubmed
Messmer,
Analysis of the functional role of the Polycomb chromo domain in Drosophila melanogaster.
1992,
Pubmed
Müller,
Function of the Polycomb protein is conserved in mice and flies.
1995,
Pubmed
Müller,
Transcriptional silencing by the Polycomb protein in Drosophila embryos.
1995,
Pubmed
Olson,
Regulated expression of Wnt family members during proliferation of C57mg mammary cells.
1994,
Pubmed
Orlando,
Mapping Polycomb-repressed domains in the bithorax complex using in vivo formaldehyde cross-linked chromatin.
1993,
Pubmed
Paro,
The Polycomb protein shares a homologous domain with a heterochromatin-associated protein of Drosophila.
1991,
Pubmed
Paro,
Imprinting a determined state into the chromatin of Drosophila.
1990,
Pubmed
Pearce,
The mouse has a Polycomb-like chromobox gene.
1992,
Pubmed
Pelegri,
A role of polycomb group genes in the regulation of gap gene expression in Drosophila.
1994,
Pubmed
Rastelli,
Related chromosome binding sites for zeste, suppressors of zeste and Polycomb group proteins in Drosophila and their dependence on Enhancer of zeste function.
1993,
Pubmed
Reijnen,
Polycomb and bmi-1 homologs are expressed in overlapping patterns in Xenopus embryos and are able to interact with each other.
1995,
Pubmed
,
Xenbase
Samuels,
Conditional transformation of cells and rapid activation of the mitogen-activated protein kinase cascade by an estradiol-dependent human raf-1 protein kinase.
1993,
Pubmed
Satijn,
RING1 is associated with the polycomb group protein complex and acts as a transcriptional repressor.
1997,
Pubmed
Schön,
Expression of protooncogenes in murine osteosarcomas.
1986,
Pubmed
Simon,
Locking in stable states of gene expression: transcriptional control during Drosophila development.
1995,
Pubmed
Singh,
A sequence motif found in a Drosophila heterochromatin protein is conserved in animals and plants.
1991,
Pubmed
Small,
Neoplastic transformation by the human gene N-myc.
1987,
Pubmed
Smith,
Measurement of protein using bicinchoninic acid.
1985,
Pubmed
Smouse,
Genetic dissection of a complex neurological mutant, polyhomeotic, in Drosophila.
1990,
Pubmed
Smouse,
polyhomeotic: a gene required for the embryonic development of axon pathways in the central nervous system of Drosophila.
1988,
Pubmed
Stone,
Definition of regions in human c-myc that are involved in transformation and nuclear localization.
1987,
Pubmed
Tagawa,
Expression of novel DNA-binding protein with zinc finger structure in various tumor cells.
1990,
Pubmed
van Lohuizen,
Identification of cooperating oncogenes in E mu-myc transgenic mice by provirus tagging.
1991,
Pubmed
Wagner,
Myc-mediated apoptosis requires wild-type p53 in a manner independent of cell cycle arrest and the ability of p53 to induce p21waf1/cip1.
1994,
Pubmed
Zink,
In vivo binding pattern of a trans-regulator of homoeotic genes in Drosophila melanogaster.
1989,
Pubmed
