Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The Xenopus oocyte is a specialized single cell of colossal size (>1 mm diameter) that is highly amenable for microinjection and a stalwart model for heterologous expression. Oocytes are easily obtainable, robust in vitro, and faithfully express injected constructs. Their large size translational capacity provides a huge canvas for observing and recording integrated cellular responses-from studies of single molecules within single cells to medium-throughput drug-screening applications. Most eukaryotic promoters suffice for Xenopus expression, and the oocyte can functionally express proteins from many diverse organisms. This protocol provides a basic introduction for scientists keen to perform nuclear microinjections of cDNA constructs. These are easy methods to master, do not require elaborate equipment, and make accessible a wonderful model cell system for studying signaling, transport, cell architecture, and protein function.
Aguero,
Microinjection of Xenopus Oocytes.
2018, Pubmed,
Xenbase
Aguero,
Microinjection of Xenopus Oocytes.
2018,
Pubmed
,
Xenbase
Cohen,
Microinjection of Xenopus laevis oocytes.
2009,
Pubmed
,
Xenbase
Cristofori-Armstrong,
Xenopus borealis as an alternative source of oocytes for biophysical and pharmacological studies of neuronal ion channels.
2015,
Pubmed
,
Xenbase
Delpire,
Housing and husbandry of Xenopus laevis affect the quality of oocytes for heterologous expression studies.
2011,
Pubmed
,
Xenbase
Lin-Moshier,
The Xenopus oocyte: a single-cell model for studying Ca2+ signaling.
2013,
Pubmed
,
Xenbase
Lin-Moshier,
Nuclear microinjection to assess how heterologously expressed proteins impact Ca2+ signals in Xenopus oocytes.
2013,
Pubmed
,
Xenbase
Lin-Moshier,
A rapid Western blotting protocol for the Xenopus oocyte.
2013,
Pubmed
,
Xenbase
Maldifassi,
Xenopus Oocytes: Optimized Methods for Microinjection, Removal of Follicular Cell Layers, and Fast Solution Changes in Electrophysiological Experiments.
2016,
Pubmed
,
Xenbase
Marchant,
Xenopus tropicalis oocytes as an advantageous model system for the study of intracellular Ca(2+) signalling.
2001,
Pubmed
,
Xenbase
Newman,
Isolation of Xenopus Oocytes.
2018,
Pubmed
,
Xenbase
O'Connell,
Xenopus laevis oocytes infected with multi-drug-resistant bacteria: implications for electrical recordings.
2011,
Pubmed
,
Xenbase
Papke,
Working with OpusXpress: methods for high volume oocyte experiments.
2010,
Pubmed
,
Xenbase
Pearl,
Development of Xenopus resource centers: the National Xenopus Resource and the European Xenopus Resource Center.
2012,
Pubmed
,
Xenbase
Schnizler,
The roboocyte: automated cDNA/mRNA injection and subsequent TEVC recording on Xenopus oocytes in 96-well microtiter plates.
2003,
Pubmed
,
Xenbase
Sive,
Isolation of Xenopus oocytes.
2010,
Pubmed
,
Xenbase
