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EMBO J
1982 Jan 01;14:409-14. doi: 10.1002/j.1460-2075.1982.tb01183.x.
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The unmethylated state of the promoter/leader and 5'-regions of integrated adenovirus genes correlates with gene expression.
Kruczek I
,
Doerfler W
.
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An inverse correlation has been established between the levels of DNA methylation at 5'-CCGG-3' (MspI/HpaII) sites in specific genes of integrated viral DNA in adenovirus type 12 (Ad12)-transformed hamster cell lines and the extent to which these genes are expressed ( Sutter and Doerfler , 1979, 1980). In general, early genes are transcribed into mRNA, while late genes are permanently switched off in these cell lines. Adenovirus type 2 genes methylated in vitro at 5'-CCGG-3' sites are not transcribed upon microinjection into nuclei of Xenopus laevis oocytes - unmethylated genes are expressed ( Vardimon et al., 1982a ). The MspI sites in the early and in some of the late Ad12 genes in cell lines HA12 /7, T637 , and A2497 -3 have now been precisely mapped. The data presented here reveal that the promoter/leader and 5'-regions of the early genes are unmethylated both at MspI sites and at 5'-GCGC-3' (HhaI) sites. In some instances, e.g., in the E2a regions in all three lines, the main parts of the early genes are partly methylated, even though the genes are expressed. In cell line HA12 /7, the early region E3 is not expressed, and the promoter/leader and 5'-regions of this segment are fully methylated. All late regions are completely methylated. The results suggest that the state of methylation in the promoter/leader and 5'-regions of integrated adenovirus genes is important in the control of gene expression.
Alwine,
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
1977, Pubmed
Alwine,
Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
1977,
Pubmed
Behe,
Effects of methylation on a synthetic polynucleotide: the B--Z transition in poly(dG-m5dC).poly(dG-m5dC).
1981,
Pubmed
Bos,
The 2.2 kb E1b mRNA of human Ad12 and Ad5 codes for two tumor antigens starting at different AUG triplets.
1981,
Pubmed
Doerfler,
DNA methylation--a regulatory signal in eukaryotic gene expression.
1981,
Pubmed
Doerfler,
Selectivity in integration sites of adenoviral DNA.
1980,
Pubmed
Ehrlich,
5-Methylcytosine in eukaryotic DNA.
1981,
Pubmed
Esche,
Expression of early viral gene products in adenovirus type 12-infected and -transformed cells.
1982,
Pubmed
Gunthert,
DNA methylation in adenovirus, adenovirus-transformed cells, and host cells.
1976,
Pubmed
Günthert,
Restriction and modification in Bacillus subtilis: two DNA methyltransferases with BsuRI specificity. II. Catalytic properties, substrate specificity, and mode of action.
1981,
Pubmed
Kuhlmann,
Shifts in the extent and patterns of DNA methylation upon explanation and subcultivation of adenovirus type 12-induced hamster tumor cells.
1982,
Pubmed
Mann,
Specificity of Hpa II and Hae III DNA methylases.
1977,
Pubmed
Ortin,
Transcription of the genome of adenovirus type 12. III. Maps of stable RNA from productively infected human cells and abortively infected and transformed hamster cells.
1976,
Pubmed
Quint,
In vitro methylation of DNA with Hpa II methylase.
1981,
Pubmed
Razin,
DNA methylation and gene function.
1980,
Pubmed
Rigby,
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
1977,
Pubmed
Schirm,
Expression of viral DNA in adenovirus type 12-transformed cells, in tumor cells, and in revertants.
1981,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Sugisaki,
Structure and gene organization in the transformed Hind III-G fragment of Ad12.
1980,
Pubmed
Sutter,
Methylation of integrated adenovirus type 12 DNA sequences in transformed cells is inversely correlated with viral gene expression.
1980,
Pubmed
Sutter,
Patterns of integration of viral DNA sequences in the genomes of adenovirus type 12-transformed hamster cells.
1978,
Pubmed
Sutter,
Methylation of integrated viral DNA sequences in hamster cells transformed by adenovirus 12.
1980,
Pubmed
Vardimon,
Expression of a cloned adenovirus gene is inhibited by in vitro methylation.
1982,
Pubmed
,
Xenbase
Vardimon,
Methylation of adenovirus genes in transformed cells and in vitro: influence on the regulation of gene expression?
1981,
Pubmed
,
Xenbase
Vardimon,
DNA methylation and viral gene expression in adenovirus-transformed and -infected cells.
1980,
Pubmed
Vogel,
Cloned fragments of human adenovirus type-12 DNA.
1981,
Pubmed
Waalwijk,
MspI, an isoschizomer of hpaII which cleaves both unmethylated and methylated hpaII sites.
1978,
Pubmed
Wahl,
Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate.
1979,
Pubmed
Wang,
Molecular structure of a left-handed double helical DNA fragment at atomic resolution.
1979,
Pubmed
Wigler,
The inheritance of methylation patterns in vertebrates.
1981,
Pubmed
