Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1987 Jan 01;61:35-41. doi: 10.1002/j.1460-2075.1987.tb04715.x.
Show Gene links
Show Anatomy links
A human and a plant intron-containing tRNATyr gene are both transcribed in a HeLa cell extract but spliced along different pathways.
van Tol H
,
Stange N
,
Gross HJ
,
Beier H
.
???displayArticle.abstract??? tRNA splicing enzymes had been identified in mammalian and plant cells long before homologous intron-containing tRNA genes were detected. The tRNATyr gene presented here is the first intron-containing, human tRNA gene for which transcription and pre-tRNA maturation has been studied in a homologous system. This gene is disrupted by a 20-bp long intron and encodes one of the two major human tRNAsTyr which have been purified and sequenced. A tRNATyr gene recently isolated from Nicotiana also contains an intron and codes for a functional, major cytoplasmic tRNATyr. Both tRNA genes are efficiently transcribed in a HeLa cell nuclear extract. Each of them produces two independent primary transcripts because of two initiation and termination sites, respectively. The maturation of the tRNATyr precursors proceeds along different pathways. The intervening sequence of the human pre-tRNATyr is excised first, followed by ligation of the tRNA halves and maturation of the flanks, as has been shown for all intron-containing tRNA genes transcribed in HeLa extract. The order of maturation steps is reversed for the plant pre-tRNATyr: processing of the flanking sequences precedes intron excision. This maturation pathway corresponds to that observed in vivo for tRNA biosynthesis in Xenopus oocytes and yeast.
Arnold,
The human tRNAVal gene family: organization, nucleotide sequences and homologous transcription of three single-copy genes.
1986, Pubmed
Arnold,
The human tRNAVal gene family: organization, nucleotide sequences and homologous transcription of three single-copy genes.
1986,
Pubmed
Beier,
UAG readthrough during TMV RNA translation: isolation and sequence of two tRNAs with suppressor activity from tobacco plants.
1984,
Pubmed
Colby,
Yeast tRNA precursor mutated at a splice junction is correctly processed in vivo.
1981,
Pubmed
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Domdey,
Studies on the primary and secondary structure of potato spindle tuber viroid: products of digestion with ribonuclease A and ribonuclease T1, and modification with bisulfite.
1978,
Pubmed
Drabkin,
Site-specific mutagenesis on a human initiator methionine tRNA gene within a sequence conserved in all eukaryotic initiator tRNAs and studies of its effects on in vitro transcription.
1985,
Pubmed
Etcheverry,
A precursor to a minor species of yeast tRNASer contains an intervening sequence.
1979,
Pubmed
Filipowicz,
Origin of splice junction phosphate in tRNAs processed by HeLa cell extract.
1983,
Pubmed
,
Xenbase
Goodman,
Nucleotide sequence of a mutant eukaryotic gene: the yeast tyrosine-inserting ochre suppressor SUP4-o.
1977,
Pubmed
Gouilloud,
A dispersed tyrosine tRNA gene from Xenopus laevis with high transcriptional activity in vitro.
1986,
Pubmed
,
Xenbase
Gruissem,
Transcription of E. coli and Euglena chloroplast tRNA gene clusters and processing of polycistronic transcripts in a HeLa cell-free system.
1982,
Pubmed
Hopper,
tRNA synthesis: identification of in vivo precursor tRNAs from parental and mutant yeast strains.
1981,
Pubmed
,
Xenbase
Johnson,
The yeast tRNATyr gene intron is essential for correct modification of its tRNA product.
1983,
Pubmed
Johnson,
The nucleotide sequence of tyrosine tRNAQ* psi A from bovine liver.
1985,
Pubmed
Koski,
Mutations of the yeast SUP4 tRNATyr locus: transcription of the mutant genes in vitro.
1980,
Pubmed
,
Xenbase
Laski,
Characterization of tRNA precursor splicing in mammalian extracts.
1983,
Pubmed
,
Xenbase
Laski,
Expression of a X. laevis tRNATyr gene in mammalian cells.
1982,
Pubmed
,
Xenbase
MacPherson,
Two human tyrosine tRNA genes contain introns.
1986,
Pubmed
Melton,
Order and intracellular location of the events involved in the maturation of a spliced tRNA.
1980,
Pubmed
,
Xenbase
Müller,
Nucleotide sequence of genes coding for tRNAPhe and tRNATyr from a repeating unit of X. laevis DNA.
1980,
Pubmed
,
Xenbase
O'Farrell,
Structure and processing of yeast precursor tRNAs containing intervening sequences.
1978,
Pubmed
Peebles,
Splicing of yeast tRNA precursors: a two-stage reaction.
1979,
Pubmed
Pirtle,
Nucleotide sequence and transcription of a human glycine tRNAGCC gene and nearby pseudogene.
1986,
Pubmed
Quigley,
Structural domains of transfer RNA molecules.
1976,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Santos,
Comparative analysis of human chromosomal segments bearing nonallelic dispersed tRNAimet genes.
1981,
Pubmed
Schaack,
Transcription of a Drosophila tRNAArg gene in yeast extract: 5'-flanking sequence dependence for transcription in a heterologous system.
1985,
Pubmed
Silberklang,
Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs.
1979,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Sprinzl,
Compilation of sequences of tRNA genes.
1985,
Pubmed
Sprinzl,
Compilation of tRNA sequences.
1985,
Pubmed
Standring,
Yeast tRNA3Leu gene transcribed and spliced in a HeLa cell extract.
1981,
Pubmed
,
Xenbase
Stange,
A gene for the major cytoplasmic tRNATyr from Nicotiana rustica contains a 13 nucleotides long intron.
1986,
Pubmed
Szostak,
Hybridization with synthetic oligonucleotides.
1979,
Pubmed
Tyc,
Ligation of endogenous tRNA 3' half molecules to their corresponding 5' halves via 2'-phosphomonoester,3',5'-phosphodiester bonds in extracts of Chlamydomonas.
1983,
Pubmed
Wallace,
Hybridization of synthetic oligodeoxyribonucleotides to phi chi 174 DNA: the effect of single base pair mismatch.
1979,
Pubmed
Woo,
The ovalbumin gene: cloning of the natural gene.
1978,
Pubmed
