Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Subfamily-specific posttranscriptional mechanism underlies K(+) channel expression in a developing neuronal blastomere.
Ono F
,
Katsuyama Y
,
Nakajo K
,
Okamura Y
.
???displayArticle.abstract???
Na(+) and K(+) channels are the two key proteins that shape the action potentials in neurons. However, little is known about how the expression of these two channels is coordinated. To address this issue, we cloned a Shab-related K(+) channel gene from ascidian Halocynthia roretzi (TuKv2). In this animal, a blastomere of neuronal lineage isolated from the 8-cell embryo expresses single Na(+) channel and K(+) channel genes after neural induction. Expression of a dominant negative form of TuKv2 eliminated the native delayed rectifier K(+) currents, indicating that the entire delayed rectifier K(+) current of the neuronal blastomere is exclusively encoded by TuKv2. TuKv2 transcripts are expressed more broadly than Na(+) channel transcripts, which are restricted to the neuronal lineages. There is also a temporal mismatch in the expression of TuKv2 transcript and the K(+) current; TuKv2 transcripts are present throughout development, whereas delayed rectifier K(+) currents only appear after the tailbud stage, suggesting that the functional expression of the TuKv2 transcript is suppressed during the early embryonic stages. To test if this suppression occurs by a mechanism specific to the TuKv2 channel protein, an ascidian Shaker-related gene, TuKv1, was misexpressed in neural blastomeres. A TuKv1-encoded current was expressed earlier than the TuKv2 current. Furthermore, the introduction of the TuKv2-expressing plasmid into noninduced cells did not lead to the current expression. These results raise the possibility that the expression of TuKv2 is post-transcriptionally controlled through a mechanism that is dependent on neural induction.
Blaine,
Heteromultimeric potassium channels formed by members of the Kv2 subfamily.
1998, Pubmed,
Xenbase
Blaine,
Heteromultimeric potassium channels formed by members of the Kv2 subfamily.
1998,
Pubmed
,
Xenbase
Burger,
Xenopus spinal neurons express Kv2 potassium channel transcripts during embryonic development.
1996,
Pubmed
,
Xenbase
Chong,
REST: a mammalian silencer protein that restricts sodium channel gene expression to neurons.
1995,
Pubmed
Dallman,
Spontaneous activity regulates calcium-dependent K+ current expression in developing ascidian muscle.
1998,
Pubmed
Dierks,
DNA sequences preceding the rabbit beta-globin gene are required for formation in mouse L cells of beta-globin RNA with the correct 5' terminus.
1981,
Pubmed
Fink,
A new K+ channel beta subunit to specifically enhance Kv2.2 (CDRK) expression.
1996,
Pubmed
,
Xenbase
Gurantz,
Temporal regulation of Shaker- and Shab-like potassium channel gene expression in single embryonic spinal neurons during K+ current development.
1996,
Pubmed
,
Xenbase
Heginbotham,
The aromatic binding site for tetraethylammonium ion on potassium channels.
1992,
Pubmed
Hirano,
Determination of excitability types in blastomeres of the cleavage-arrested but differentiated embryos of an ascidian.
1984,
Pubmed
Inazawa,
Basic fibroblast growth factor induction of neuronal ion channel expression in ascidian ectodermal blastomeres.
1998,
Pubmed
Jackson,
Two types of mutants affecting voltage-sensitive sodium channels in Drosophila melanogaster.
,
Pubmed
Jones,
Overexpression of a potassium channel gene perturbs neural differentiation.
1994,
Pubmed
,
Xenbase
Linsdell,
Na+ channel mis-expression accelerates K+ channel development in embryonic Xenopus laevis skeletal muscle.
1994,
Pubmed
,
Xenbase
MacKinnon,
Mutations affecting TEA blockade and ion permeation in voltage-activated K+ channels.
1990,
Pubmed
MacKinnon,
Determination of the subunit stoichiometry of a voltage-activated potassium channel.
1991,
Pubmed
,
Xenbase
McManus,
Functional role of the beta subunit of high conductance calcium-activated potassium channels.
1995,
Pubmed
,
Xenbase
Murakoshi,
Identification of the Kv2.1 K+ channel as a major component of the delayed rectifier K+ current in rat hippocampal neurons.
1999,
Pubmed
Nakahira,
Selective interaction of voltage-gated K+ channel beta-subunits with alpha-subunits.
1996,
Pubmed
Nishida,
Cell lineage analysis in ascidian embryos by intracellular injection of a tracer enzyme. III. Up to the tissue restricted stage.
1987,
Pubmed
O'Dowd,
Voltage-clamp analysis of sodium channels in wild-type and mutant Drosophila neurons.
1988,
Pubmed
Okada,
Distinct neuronal lineages of the ascidian embryo revealed by expression of a sodium channel gene.
1997,
Pubmed
Okado,
Neural differentiation in cleavage-arrested ascidian blastomeres induced by a proteolytic enzyme.
1993,
Pubmed
Okado,
Induced neural-type differentiation in the cleavage-arrested blastomere isolated from early ascidian embryos.
1990,
Pubmed
Okamura,
Neural induction suppresses early expression of the inward-rectifier K+ channel in the ascidian blastomere.
1993,
Pubmed
Okamura,
Neural expression of a sodium channel gene requires cell-specific interactions.
1994,
Pubmed
Okamura,
Changes in sodium channels during neural differentiation in the isolated blastomere of the ascidian embryo.
1990,
Pubmed
Perozo,
Gating currents from a nonconducting mutant reveal open-closed conformations in Shaker K+ channels.
1993,
Pubmed
Pongs,
Molecular biology of voltage-dependent potassium channels.
1992,
Pubmed
Quattrocki,
A Shab potassium channel contributes to action potential broadening in peptidergic neurons.
1994,
Pubmed
,
Xenbase
Rettig,
Inactivation properties of voltage-gated K+ channels altered by presence of beta-subunit.
1994,
Pubmed
,
Xenbase
Ribera,
Homogeneous development of electrical excitability via heterogeneous ion channel expression.
1996,
Pubmed
,
Xenbase
Saitoe,
Neuronal expression in cleavage-arrested ascidian blastomeres requires gap junctional uncoupling from neighbouring cells.
1996,
Pubmed
Sakakibara,
Expression of neural RNA-binding proteins in the postnatal CNS: implications of their roles in neuronal and glial cell development.
1997,
Pubmed
Shen,
Molecular recognition and assembly sequences involved in the subfamily-specific assembly of voltage-gated K+ channel subunit proteins.
1995,
Pubmed
Shi,
Beta subunits promote K+ channel surface expression through effects early in biosynthesis.
1996,
Pubmed
Shidara,
Developmental changes in delayed rectifier K+ currents in the muscular- and neural-type blastomere of ascidian embryos.
1991,
Pubmed
Simoncini,
Lineage-specific development of calcium currents during embryogenesis.
1988,
Pubmed
Takahashi,
Ion channels and early development of neural cells.
1998,
Pubmed
Tsunoda,
The major delayed rectifier in both Drosophila neurons and muscle is encoded by Shab.
1995,
Pubmed
Tsunoda,
Genetic analysis of Drosophila neurons: Shal, Shaw, and Shab encode most embryonic potassium currents.
1995,
Pubmed
Vize,
Assays for gene function in developing Xenopus embryos.
1991,
Pubmed
,
Xenbase
Wada,
Phylogenetic Relationships between Solitary and Colonial Ascidians, as Inferred from the Sequence of the Central Region of their Respective 18S rDNAs.
1992,
Pubmed
Wang,
The seizure locus encodes the Drosophila homolog of the HERG potassium channel.
1997,
Pubmed
Wible,
Cloning and expression of a novel K+ channel regulatory protein, KChAP.
1998,
Pubmed
,
Xenbase
Xu,
Assembly of voltage-gated potassium channels. Conserved hydrophilic motifs determine subfamily-specific interactions between the alpha-subunits.
1995,
Pubmed
Yang,
How does the W434F mutation block current in Shaker potassium channels?
1997,
Pubmed
,
Xenbase
