Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1990 Dec 01;912:4083-94. doi: 10.1002/j.1460-2075.1990.tb07630.x.
Show Gene links
Show Anatomy links
Structure of an invertebrate gene encoding cytoplasmic intermediate filament (IF) proteins: implications for the origin and the diversification of IF proteins.
Dodemont H
,
Riemer D
,
Weber K
.
???displayArticle.abstract???
The structure of the single gene encoding the cytoplasmic intermediate filament (IF) proteins in non-neuronal cells of the gastropod Helix aspersa is described. Genomic and cDNA sequences show that the gene is composed of 10 introns and 11 exons, spanning greater than 60 kb of DNA. Alternative RNA processing accounts for two mRNA families which encode two IF proteins differing only in their C-terminal sequence. The intron/exon organization of the Helix rod domain is identical to that of the vertebrate type III IF genes in spite of low overall protein sequence homology and the presence of an additional 42 residues in coil 1b of the invertebrate sequence. Intron position homology extends to the entire coding sequence comprising both the rod and tail domains when the invertebrate IF gene is compared with the nuclear lamin LIII gene of Xenopus laevis presented in the accompanying report of Döring and Stick. In contrast the intron patterns of the tail domains of the invertebrate IF and the lamin genes differ from those of the vertebrate type III genes. The combined data are in line with an evolutionary descent of cytoplasmic IF proteins from a nuclear lamin-like progenitor and suggest a mechanism for this derivation. The unique position of intron 7 in the Helix IF gene indicates that the archetype IF gene arose by the elimination of the nuclear localization sequence due to the recruitment of a novel splice site. The presumptive structural organization of the archetype IF gene allows predictions with respect to the later diversification of metazoan IF genes. Whereas models proposing a direct derivation of neurofilament genes seem unlikely, the earlier speculation of an mRNA transposition mechanism is compatible with current results.
Aebi,
The nuclear lamina is a meshwork of intermediate-type filaments.
, Pubmed,
Xenbase
Aebi,
The nuclear lamina is a meshwork of intermediate-type filaments.
,
Pubmed
,
Xenbase
Alt,
Synthesis of secreted and membrane-bound immunoglobulin mu heavy chains is directed by mRNAs that differ at their 3' ends.
1980,
Pubmed
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed
Bader,
Amino acid sequence and gene organization of cytokeratin no. 19, an exceptional tail-less intermediate filament protein.
1986,
Pubmed
Bailey,
Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
1976,
Pubmed
Balcarek,
Structure of the mouse glial fibrillary acidic protein gene: implications for the evolution of the intermediate filament multigene family.
1985,
Pubmed
Bartnik,
Intermediate filaments in non-neuronal cells of invertebrates: isolation and biochemical characterization of intermediate filaments from the esophageal epithelium of the mollusc Helix pomatia.
1985,
Pubmed
BEAMS,
Ultrastructure of the nuclear membrane of a gregarine parasitic in grasshoppers.
1957,
Pubmed
Birnstiel,
Transcription termination and 3' processing: the end is in site!
1985,
Pubmed
Brawerman,
Determinants of messenger RNA stability.
1987,
Pubmed
Breathnach,
Organization and expression of eucaryotic split genes coding for proteins.
1981,
Pubmed
Casey,
Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide.
1977,
Pubmed
Dibb,
Evidence that introns arose at proto-splice sites.
1989,
Pubmed
Dodemont,
Comparison of the crystallin mRNA populations from rat, calf and duck lens. Evidence for a longer alpha A2-mRNA and two distinct alpha B2-mRNAs in the birds.
1985,
Pubmed
Döring,
Gene structure of nuclear lamin LIII of Xenopus laevis; a model for the evolution of IF proteins from a lamin-like ancestor.
1990,
Pubmed
,
Xenbase
Early,
Two mRNAs can be produced from a single immunoglobulin mu gene by alternative RNA processing pathways.
1980,
Pubmed
Fawcett,
On the occurrence of a fibrous lamina on the inner aspect of the nuclear envelope in certain cells of vertebrates.
1966,
Pubmed
Fisher,
cDNA sequencing of nuclear lamins A and C reveals primary and secondary structural homology to intermediate filament proteins.
1986,
Pubmed
Geisler,
The amino acid sequence of chicken muscle desmin provides a common structural model for intermediate filament proteins.
1982,
Pubmed
Georgatos,
Lamin A, lamin B, and lamin B receptor analogues in yeast.
1989,
Pubmed
Gruenbaum,
Drosophila nuclear lamin precursor Dm0 is translated from either of two developmentally regulated mRNA species apparently encoded by a single gene.
1988,
Pubmed
Gubler,
A simple and very efficient method for generating cDNA libraries.
1983,
Pubmed
Hanahan,
Plasmid screening at high colony density.
1980,
Pubmed
Hancock,
All ras proteins are polyisoprenylated but only some are palmitoylated.
1989,
Pubmed
Hargreaves,
Reconstitution of intermediate filaments from a higher plant.
1989,
Pubmed
Hennessy,
Complete thrombospondin mRNA sequence includes potential regulatory sites in the 3' untranslated region.
1989,
Pubmed
Holtz,
The CaaX motif of lamin A functions in conjunction with the nuclear localization signal to target assembly to the nuclear envelope.
1989,
Pubmed
Ichinose,
Nucleotide sequence and structure of the mouse cytokeratin endoB gene.
1988,
Pubmed
Johnson,
Structure of a gene for the human epidermal 67-kDa keratin.
1985,
Pubmed
Julien,
The structure of a human neurofilament gene (NF-L): a unique exon-intron organization in the intermediate filament gene family.
1987,
Pubmed
Julien,
Sequence and structure of the mouse gene coding for the largest neurofilament subunit.
1988,
Pubmed
Kozak,
The scanning model for translation: an update.
1989,
Pubmed
Krauss,
Organization and sequence of the human gene encoding cytokeratin 8.
1990,
Pubmed
,
Xenbase
Krieg,
Organization of a type I keratin gene. Evidence for evolution of intermediate filaments from a common ancestral gene.
1985,
Pubmed
Lees,
The structure and organization of the human heavy neurofilament subunit (NF-H) and the gene encoding it.
1988,
Pubmed
Leff,
Complex transcriptional units: diversity in gene expression by alternative RNA processing.
1986,
Pubmed
Lendahl,
CNS stem cells express a new class of intermediate filament protein.
1990,
Pubmed
Lewis,
Anomalous placement of introns in a member of the intermediate filament multigene family: an evolutionary conundrum.
1986,
Pubmed
Loewinger,
Mutations in the nuclear lamin proteins resulting in their aberrant assembly in the cytoplasm.
1988,
Pubmed
Marchuk,
Remarkable conservation of structure among intermediate filament genes.
1984,
Pubmed
McKeon,
Homologies in both primary and secondary structure between nuclear envelope and intermediate filament proteins.
,
Pubmed
MERCER,
An electron microscopic study of Amoeba proteus.
1959,
Pubmed
Mitchell,
Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins.
1989,
Pubmed
Myers,
The human mid-size neurofilament subunit: a repeated protein sequence and the relationship of its gene to the intermediate filament gene family.
1987,
Pubmed
Padgett,
Splicing of messenger RNA precursors.
1986,
Pubmed
Palmiter,
Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.
1974,
Pubmed
PAPPAS,
The fine structure of the nuclear envelope of Amoeba proteus.
1956,
Pubmed
Peacock,
Transformation of E. coli using homopolymer-linked plasmid chimeras.
1981,
Pubmed
Pollard,
In vitro posttranslational modification of lamin B cloned from a human T-cell line.
1990,
Pubmed
Proudfoot,
3' non-coding region sequences in eukaryotic messenger RNA.
1976,
Pubmed
Quax,
The structure of the vimentin gene.
1983,
Pubmed
Quax,
Characterization of the hamster desmin gene: expression and formation of desmin filaments in nonmuscle cells after gene transfer.
1985,
Pubmed
Rigby,
Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
1977,
Pubmed
Rogers,
Two mRNAs with different 3' ends encode membrane-bound and secreted forms of immunoglobulin mu chain.
1980,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Shaw,
A conserved AU sequence from the 3' untranslated region of GM-CSF mRNA mediates selective mRNA degradation.
1986,
Pubmed
Sippel,
Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli.
1973,
Pubmed
Southern,
Detection of specific sequences among DNA fragments separated by gel electrophoresis.
1975,
Pubmed
Steinert,
Intermediate filament dynamics.
1990,
Pubmed
Steinert,
Molecular and cellular biology of intermediate filaments.
1988,
Pubmed
Thomas,
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
1980,
Pubmed
Thompson,
Structure of the gene encoding peripherin, an NGF-regulated neuronal-specific type III intermediate filament protein.
1989,
Pubmed
Tyner,
The sequence of a type II keratin gene expressed in human skin: conservation of structure among all intermediate filament genes.
1985,
Pubmed
Vorburger,
Modification of nuclear lamin proteins by a mevalonic acid derivative occurs in reticulocyte lysates and requires the cysteine residue of the C-terminal CXXM motif.
1989,
Pubmed
Weber,
Cytoplasmic intermediate filament proteins of invertebrates are closer to nuclear lamins than are vertebrate intermediate filament proteins; sequence characterization of two muscle proteins of a nematode.
1989,
Pubmed
Weber,
Amino acid sequences and homopolymer-forming ability of the intermediate filament proteins from an invertebrate epithelium.
1988,
Pubmed
Wilson,
Removal of poly(A) and consequent degradation of c-fos mRNA facilitated by 3' AU-rich sequences.
1988,
Pubmed
Yanisch-Perron,
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
1985,
Pubmed
