Kondaiah P et al. (2000), Transforming growth factor-beta5 expression dur...

XB-ART-10639

Mech Dev 2000 Jul 01;951-2:207-9. doi: 10.1016/s0925-4773(00)00326-9.

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Transforming growth factor-beta5 expression during early development of Xenopus laevis.

Kondaiah P , Taira M , Vempati UD , Dawid IB .

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Members of the transforming growth factor-beta (TGF-beta) superfamily play various roles during development in both vertebrates and invertebrates. Two isoforms, TGF-beta2 and -beta5, have been isolated from Xenopus laevis. We describe here the localization of TGF-beta5 mRNA in early embryos of X. laevis, assessed by whole-mount in situ hybridization. The first detectable expression of TGF-beta5 was seen in the stage 14 embryo at the posterior tip of notochord, which continued to later stages, accompanied by the expression in bilateral regions of posterior wall in the tail region next to the notochord. At later stages, transient expression was seen in the cement gland (around stage 21) and in the somites (stages 24-27). In addition, expression was present in the branchial arches (stage 29-36) and olfactory placodes (stage 36).

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Species referenced: Xenopus laevis
Genes referenced: tgfb1

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	tgfb1 (transforming growth factor, beta 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 14, dorsal view, anterior left.
	tgfb1 (transforming growth factor, beta 1) gene expression in bisected Xenopus laevis embryo, parasagital section, assayed via in situ hybridization, NF stage 21, dorsal up, anterior right

	tgfb1 (transforming growth factor, beta 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 27, lateral view, anterior left, dorsal up.
	tgfb1 (transforming growth factor, beta 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 27, dorsal view, anterior left.

	tgfb1 (transforming growth factor, beta 1) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 42, lateral view, anterior left, dorsal up.
	Fig. 1. The developmental expression of TGF-b5 in Xenopus laevis. Anterior is to the left except in C, G. Cleared embryos (A,B,F,G,I,J). (A) Stage 14; a dorsal view shows expression at the posterior end of the notochord. (B,C) Stage 21; lateral (B) anterior front (C) views show strong staining in the cement gland anlage. (D) Stage 25; lateral view, staining in the somites. (E) Stage 27. (E) Lateral view, staining in somites and in the tail bud. (F) Dorsal view of a cleared embryo; staining in the somites, the posterior tip of notochord, and posterior bilateral regions next to the notochord. (G) Posterior view of the embryo shown in (F), focussed on the tail bud region. (H) Stage 36; a lateral view; showing staining in the branchial arches and tail region. (I) Stage 42; a lateral view; intense staining in the head region. (J) Dorsal view of the head region shown in I with intense staining of the branchial arches and olfactory placodes. ba, branchial arches; cg, cement gland; n, notochord; op, olfactory placodes; sm, somites.