Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Interaction between constitutively expressed heat shock protein, Hsc 70, and cysteine string protein is important for cortical granule exocytosis in Xenopus oocytes.
???displayArticle.abstract???
In many species, binding of sperm to the egg initiates cortical granule exocytosis, an event that contributes to a sustained block of polyspermy. Interestingly, cortical granule exocytosis can be elicited in immature Xenopus oocytes by the protein kinase C activator, phorbol-12-myristate-13-acetate. In this study, we investigated the role of cysteine string protein (csp) in phorbol-12-myristate-13-acetate-evoked cortical granule exocytosis. Prior work indicated that csp is associated with cortical granules of Xenopus oocytes. In oocytes exhibiting >20-fold overexpression of full-length Xenopus csp, cortical granule exocytosis was reduced by approximately 80%. However, csp overexpression did not affect constitutive exocytosis. Subcellular fractionation and confocal fluorescence microscopy revealed that little or none of the overexpressed csp was associated with cortical granules. This accumulation of csp at sites other than cortical granules suggested that mislocalized csp might sequester a protein that is important for regulated exocytosis. Because the NH2-terminal region of csp includes a J-domain, which interacts with constitutively expressed 70-kDa heat shock proteins (Hsc 70), we evaluated the effect of overexpressing the J-domain of csp. Although the native J-domain of csp inhibited cortical granule exocytosis, point mutations that interfere with J-domain binding to Hsc 70 eliminated this inhibition. These data indicate that csp interaction with Hsc 70 molecular chaperones is vital for regulated secretion in Xenopus oocytes.
Ali,
Isolation and characterization of a cDNA encoding a Xenopus 70-kDa heat shock cognate protein, Hsc70.I.
1996, Pubmed,
Xenbase
Ali,
Isolation and characterization of a cDNA encoding a Xenopus 70-kDa heat shock cognate protein, Hsc70.I.
1996,
Pubmed
,
Xenbase
Ali,
Molecular cloning of a cDNA encoding a Xenopus laevis 70-kDa heat shock cognate protein, hsc70.II.
1996,
Pubmed
,
Xenbase
Arnold,
Structure-function analysis of the cysteine string protein in Drosophila: cysteine string, linker and C terminus.
2004,
Pubmed
Augustine,
Exocytosis: proteins and perturbations.
1996,
Pubmed
Bement,
Protein kinase C acts downstream of calcium at entry into the first mitotic interphase of Xenopus laevis.
1990,
Pubmed
,
Xenbase
Bement,
Activators of protein kinase C trigger cortical granule exocytosis, cortical contraction, and cleavage furrow formation in Xenopus laevis oocytes and eggs.
1989,
Pubmed
,
Xenbase
Bennett,
Syntaxin: a synaptic protein implicated in docking of synaptic vesicles at presynaptic active zones.
1992,
Pubmed
Boal,
The variable C-terminus of cysteine string proteins modulates exocytosis and protein-protein interactions.
2004,
Pubmed
Braun,
The cysteine string secretory vesicle protein activates Hsc70 ATPase.
1996,
Pubmed
Brown,
Cysteine string protein (CSP) is an insulin secretory granule-associated protein regulating beta-cell exocytosis.
1998,
Pubmed
Buchner,
The DnaJ-like cysteine string protein and exocytotic neurotransmitter release.
1997,
Pubmed
Burgoyne,
Regulated exocytosis.
1993,
Pubmed
Chamberlain,
Cysteine-string protein: the chaperone at the synapse.
2000,
Pubmed
,
Xenbase
Chamberlain,
Cysteine string protein functions directly in regulated exocytosis.
1998,
Pubmed
Chamberlain,
Activation of the ATPase activity of heat-shock proteins Hsc70/Hsp70 by cysteine-string protein.
1997,
Pubmed
Chamberlain,
The molecular chaperone function of the secretory vesicle cysteine string proteins.
1997,
Pubmed
Charbonneau,
The onset of activation responsiveness during maturation coincides with the formation of the cortical endoplasmic reticulum in oocytes of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Chen,
Enhancement of presynaptic calcium current by cysteine string protein.
2002,
Pubmed
Cordeiro,
Convergent effects of lithium and valproate on the expression of proteins associated with large dense core vesicles in NGF-differentiated PC12 cells.
2004,
Pubmed
Dawson-Scully,
Cysteine-string protein increases the calcium sensitivity of neurotransmitter exocytosis in Drosophila.
2000,
Pubmed
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Graham,
Comparison of cysteine string protein (Csp) and mutant alpha-SNAP overexpression reveals a role for csp in late steps of membrane fusion in dense-core granule exocytosis in adrenal chromaffin cells.
2000,
Pubmed
Gundersen,
Activation of protein kinase Ceta triggers cortical granule exocytosis in Xenopus oocytes.
2002,
Pubmed
,
Xenbase
Gundersen,
Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes.
,
Pubmed
,
Xenbase
Gundersen,
Extensive lipidation of a Torpedo cysteine string protein.
1994,
Pubmed
,
Xenbase
Gundersen,
Cysteine-string proteins as templates for membrane fusion: models of synaptic vesicle exocytosis.
1995,
Pubmed
Gundersen,
Suppression cloning of the cDNA for a candidate subunit of a presynaptic calcium channel.
1992,
Pubmed
,
Xenbase
Gundersen,
Cysteine string proteins are associated with cortical granules of Xenopus laevis oocytes.
2001,
Pubmed
,
Xenbase
Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase
Herberts,
Immunolocalization of HSP 70-related proteins constitutively expressed during Xenopus laevis oogenesis and development.
1993,
Pubmed
,
Xenbase
Kelley,
The J-domain family and the recruitment of chaperone power.
1998,
Pubmed
Klausner,
Brefeldin A: insights into the control of membrane traffic and organelle structure.
1992,
Pubmed
Kohan,
Protein synthesis is required for the transition to Ca(2+)-dependent regulated secretion in progesterone-matured Xenopus oocytes.
2003,
Pubmed
,
Xenbase
Lane,
The Xenopus oocyte as a surrogate secretory system. The specificity of protein export.
1980,
Pubmed
,
Xenbase
Leveque,
Interaction of cysteine string proteins with the alpha1A subunit of the P/Q-type calcium channel.
1998,
Pubmed
Mandell,
Identification of two HSP70-related Xenopus oocyte proteins that are capable of recycling across the nuclear envelope.
1990,
Pubmed
,
Xenbase
Mastrogiacomo,
A Xenopus cysteine string protein with a cysteine residue in the J domain.
1998,
Pubmed
,
Xenbase
Poage,
Antibodies against cysteine string proteins inhibit evoked neurotransmitter release at Xenopus neuromuscular junctions.
1999,
Pubmed
,
Xenbase
Stahl,
Two distinct domains in hsc70 are essential for the interaction with the synaptic vesicle cysteine string protein.
1999,
Pubmed
Tobaben,
A trimeric protein complex functions as a synaptic chaperone machine.
2001,
Pubmed
Umbach,
Attenuated influx of calcium ions at nerve endings of csp and shibire mutant Drosophila.
1998,
Pubmed
Umbach,
Evidence that cysteine string proteins regulate an early step in the Ca2+-dependent secretion of neurotransmitter at Drosophila neuromuscular junctions.
1997,
Pubmed
Umbach,
Presynaptic dysfunction in Drosophila csp mutants.
1994,
Pubmed
Zampighi,
Functional and morphological correlates of connexin50 expressed in Xenopus laevis oocytes.
1999,
Pubmed
,
Xenbase
Zhang,
Cysteine-string proteins regulate exocytosis of insulin independent from transmembrane ion fluxes.
1998,
Pubmed
Zhang,
Mutational analysis of cysteine-string protein function in insulin exocytosis.
1999,
Pubmed
Zinsmaier,
Molecular chaperones and the regulation of neurotransmitter exocytosis.
2001,
Pubmed
Zinsmaier,
A cysteine-string protein is expressed in retina and brain of Drosophila.
1990,
Pubmed
