XB-ART-37067
Mol Cell Biol
2008 Mar 01;285:1755-69. doi: 10.1128/MCB.01697-07.
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Centrin 2 localizes to the vertebrate nuclear pore and plays a role in mRNA and protein export.
Resendes KK
,
Rasala BA
,
Forbes DJ
.
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Centrins in vertebrates have traditionally been associated with microtubule-nucleating centers such as the centrosome. Unexpectedly, we found centrin 2 to associate biochemically with nucleoporins, including the Xenopus laevis Nup107-160 complex, a critical subunit of the vertebrate nuclear pore in interphase and of the kinetochores and spindle poles in mitosis. Immunofluorescence of Xenopus cells and in vitro reconstituted nuclei indeed revealed centrin 2 localized at the nuclear pores. Use of the mild detergent digitonin in immunofluorescence also allowed centrin 2 to be clearly visualized at the nuclear pores of human cells. Disruption of nuclear pores using RNA interference of the pore assembly protein ELYS/MEL-28 resulted in a specific decrease of centrin 2 at the nuclear rim of HeLa cells. Functionally, excess expression of either the N- or C-terminal calcium-binding domains of human centrin 2 caused a dominant-negative effect on both mRNA and protein export, leaving protein import intact. The mRNA effect mirrors that found for the Saccharomyes cerevisiae centrin Cdc31p at the yeast nuclear pore, a role until now thought to be unique to yeast. We conclude that in vertebrates, centrin 2 interacts with major subunits of the nuclear pore, exhibits nuclear pore localization, and plays a functional role in multiple nuclear export pathways.
???displayArticle.pubmedLink??? 18172010
???displayArticle.pmcLink??? PMC2258798
???displayArticle.link??? Mol Cell Biol
???displayArticle.grants??? [+]
GM 68524 NIGMS NIH HHS , R01 GM-33279 NIGMS NIH HHS , R01 GM033279-24 NIGMS NIH HHS , R01 GM033279 NIGMS NIH HHS , K12 GM068524 NIGMS NIH HHS
Species referenced: Xenopus laevis
Genes referenced: mtnr1al nup107
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