Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1994 Aug 01;148:5425-32. doi: 10.1128/mcb.14.8.5425-5432.1994.
Show Gene links
Show Anatomy links
The cytoplasm of Xenopus oocytes contains a factor that protects double-stranded RNA from adenosine-to-inosine modification.
Saccomanno L
,
Bass BL
.
???displayArticle.abstract???
Here we describe studies of double-stranded RNA (dsRNA) adenosine deaminase in Xenopus laevis, in particular during meiotic maturation, the period during which a stage VI oocyte matures to an egg. We show that dsRNA adenosine deaminase is in the nuclei of stage VI oocytes. Most importantly, we demonstrate that the cytoplasm of stage VI oocytes contains a factor that protects microinjected dsRNA from deamination when dsRNA adenosine deaminase is released from the nucleus during meiotic maturation. Our data suggest that the protection factor is a cytoplasmic dsRNA-binding protein or proteins that bind to dsRNA in a sequence-independent manner to occlude dsRNA from binding to dsRNA adenosine deaminase. The cytoplasmic double-stranded RNA-binding protein(s) does not bind to other nucleic acids and can be titrated at high concentrations of dsRNA. These studies raise the question of whether all dsRNA-binding proteins share endogenous substrates and also suggest potential means of regulating dsRNA adenosine deaminase in vivo.
Bass,
Binding properties of newly identified Xenopus proteins containing dsRNA-binding motifs.
1994,
Pubmed
,
Xenbase
Bass,
An unwinding activity that covalently modifies its double-stranded RNA substrate.
1988,
Pubmed
,
Xenbase
Bass,
A developmentally regulated activity that unwinds RNA duplexes.
1987,
Pubmed
,
Xenbase
Chang,
The E3L gene of vaccinia virus encodes an inhibitor of the interferon-induced, double-stranded RNA-dependent protein kinase.
1992,
Pubmed
Feldherr,
Manual enucleation of Xenopus oocytes.
1978,
Pubmed
,
Xenbase
Fu,
Translational potentiation of messenger RNA with secondary structure in Xenopus.
1991,
Pubmed
,
Xenbase
Gatignol,
Relatedness of an RNA-binding motif in human immunodeficiency virus type 1 TAR RNA-binding protein TRBP to human P1/dsI kinase and Drosophila staufen.
1993,
Pubmed
Harland,
Translation of mRNA injected into Xenopus oocytes is specifically inhibited by antisense RNA.
1985,
Pubmed
,
Xenbase
Higuchi,
RNA editing of AMPA receptor subunit GluR-B: a base-paired intron-exon structure determines position and efficiency.
1993,
Pubmed
Hough,
Purification of the Xenopus laevis double-stranded RNA adenosine deaminase.
1994,
Pubmed
,
Xenbase
Imani,
Inhibitory activity for the interferon-induced protein kinase is associated with the reovirus serotype 1 sigma 3 protein.
1988,
Pubmed
Kim,
Double-stranded RNA adenosine deaminase as a potential mammalian RNA editing factor.
1993,
Pubmed
Kimelman,
An antisense mRNA directs the covalent modification of the transcript encoding fibroblast growth factor in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Lloyd,
Translational stimulation by reovirus polypeptide sigma 3: substitution for VAI RNA and inhibition of phosphorylation of the alpha subunit of eukaryotic initiation factor 2.
1992,
Pubmed
Milligan,
Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates.
1987,
Pubmed
Nishikura,
Substrate specificity of the dsRNA unwinding/modifying activity.
1991,
Pubmed
Polson,
The mechanism of adenosine to inosine conversion by the double-stranded RNA unwinding/modifying activity: a high-performance liquid chromatography-mass spectrometry analysis.
1991,
Pubmed
Rebagliati,
Antisense RNA injections in fertilized frog eggs reveal an RNA duplex unwinding activity.
1987,
Pubmed
,
Xenbase
Samuel,
Antiviral actions of interferon. Interferon-regulated cellular proteins and their surprisingly selective antiviral activities.
1991,
Pubmed
Sharmeen,
Tat-dependent adenosine-to-inosine modification of wild-type transactivation response RNA.
1991,
Pubmed
,
Xenbase
Skeiky,
Developmental regulation of covalent modification of double-stranded RNA during silkmoth oogenesis.
1991,
Pubmed
Smith,
The induction of oocyte maturation: transmembrane signaling events and regulation of the cell cycle.
1989,
Pubmed
St Johnston,
A conserved double-stranded RNA-binding domain.
1992,
Pubmed
,
Xenbase
Wagner,
Double-stranded RNA unwinding and modifying activity is detected ubiquitously in primary tissues and cell lines.
1990,
Pubmed
,
Xenbase
Wagner,
Cell cycle expression of RNA duplex unwindase activity in mammalian cells.
1988,
Pubmed
,
Xenbase
