Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Cell type-specific relationships between spiking and [Ca2+]i in neurons of the Xenopus tadpoleolfactory bulb.
Lin BJ
,
Chen TW
,
Schild D
.
???displayArticle.abstract???
Multi-neuronal recordings with Ca2+ indicator dyes usually relate [Ca2+]i to action potentials (APs) assuming a stereotypical dependency between the two. However, [Ca2+]i affects and is affected by numerous complex mechanisms that differ from cell type to cell type, from cell compartment to cell compartment. Moreover, [Ca2+]i depends on the specific way a cell is activated. Here we investigate, by combining calcium imaging and on-cell patch clamp recordings, the relationship between APs (spiking) and somatic [Ca2+]i in mitral and granule cells of the olfactory bulb in Xenopus laevis tadpoles. Both cell types exhibit ongoing and odour-modulated [Ca2+]i dynamics. In mitral cells, the occurrence of APs in both spontaneous and odour-evoked situations correlates tightly to step-like [Ca2+]i increases. Moreover, odorant-induced suppression of spontaneous firing couples to a decrease in [Ca2+]i. In contrast, granule cells show a substantial number of uncorrelated events such as increases in [Ca2+]i without APs occurring or APs without any effect upon [Ca2+]i. The correlation between spiking and [Ca2+]i is low, possibly due to somatic NMDAR-mediated and subthreshold voltage-activated Ca2+ entries, and thus does not allow a reliable prediction of APs based on calcium imaging. Taken together, our results demonstrate that the relationship between somatic [Ca2+]i and APs can be cell type specific. Taking [Ca2+]i dynamics as an indicator for spiking activity is thus only reliable if the correlation has been established in the system of interest. When [Ca2+]i and APs are precisely correlated, fast calcium imaging is an extremely valuable tool for determining spatiotemporal patterns of APs in neuronal population.
Alonso,
Chemical organization of the macaque monkey olfactory bulb: II. Calretinin, calbindin D-28k, parvalbumin, and neurocalcin immunoreactivity.
2001, Pubmed
Alonso,
Chemical organization of the macaque monkey olfactory bulb: II. Calretinin, calbindin D-28k, parvalbumin, and neurocalcin immunoreactivity.
2001,
Pubmed
Alvarez-Buylla,
Neurogenesis in adult subventricular zone.
2002,
Pubmed
Bullen,
High-speed, random-access fluorescence microscopy: II. Fast quantitative measurements with voltage-sensitive dyes.
1999,
Pubmed
Byrd,
Development of the olfactory bulb in the clawed frog, Xenopus laevis: a morphological and quantitative analysis.
1991,
Pubmed
,
Xenbase
Cang,
In vivo whole-cell recording of odor-evoked synaptic transmission in the rat olfactory bulb.
2003,
Pubmed
Carleton,
Becoming a new neuron in the adult olfactory bulb.
2003,
Pubmed
Chen,
In situ background estimation in quantitative fluorescence imaging.
2006,
Pubmed
,
Xenbase
Czesnik,
Noradrenergic modulation of calcium currents and synaptic transmission in the olfactory bulb of Xenopus laevis tadpoles.
2001,
Pubmed
,
Xenbase
Czesnik,
Neuronal representation of odourants in the olfactory bulb of Xenopus laevis tadpoles.
2003,
Pubmed
,
Xenbase
Egger,
Mechanisms of lateral inhibition in the olfactory bulb: efficiency and modulation of spike-evoked calcium influx into granule cells.
2003,
Pubmed
Egger,
Imaging the activity of neuronal populations: when spikes don't flash and flashes don't spike.
2007,
Pubmed
,
Xenbase
Egger,
Dendrodendritic synaptic signals in olfactory bulb granule cells: local spine boost and global low-threshold spike.
2005,
Pubmed
Hinds,
Autoradiographic study of histogenesis in the mouse olfactory bulb. I. Time of origin of neurons and neuroglia.
1968,
Pubmed
Hinds,
Autoradiographic study of histogenesis in the mouse olfactory bulb. II. Cell proliferation and migration.
1968,
Pubmed
Isaacson,
Glutamate-mediated extrasynaptic inhibition: direct coupling of NMDA receptors to Ca(2+)-activated K+ channels.
2001,
Pubmed
Iyer,
Fast functional imaging of single neurons using random-access multiphoton (RAMP) microscopy.
2006,
Pubmed
Kapoor,
Glomerulus-specific, long-latency activity in the olfactory bulb granule cell network.
2006,
Pubmed
Kovalchuk,
NMDA receptor-mediated subthreshold Ca(2+) signals in spines of hippocampal neurons.
2000,
Pubmed
Kuba,
Intracellular Ca2+ dynamics in response to Ca2+ influx and Ca2+ release in autonomic neurones.
1992,
Pubmed
Laurent,
A systems perspective on early olfactory coding.
1999,
Pubmed
López-Mascaraque,
Structure of the olfactory bulb of the hedgehog (Erinaceus europaeus): description of cell types in the granular layer.
1986,
Pubmed
Markram,
Dendritic calcium transients evoked by single back-propagating action potentials in rat neocortical pyramidal neurons.
1995,
Pubmed
Nezlin,
Structure of the olfactory bulb in tadpoles of Xenopus laevis.
2000,
Pubmed
,
Xenbase
Ohki,
Functional imaging with cellular resolution reveals precise micro-architecture in visual cortex.
2005,
Pubmed
Perez-Reyes,
Molecular physiology of low-voltage-activated t-type calcium channels.
2003,
Pubmed
Pinato,
Dendritic sodium spikelets and low-threshold calcium spikes in turtle olfactory bulb granule cells.
2005,
Pubmed
Pinato,
Regulation of granule cell excitability by a low-threshold calcium spike in turtle olfactory bulb.
2003,
Pubmed
Pressler,
Blanes cells mediate persistent feedforward inhibition onto granule cells in the olfactory bulb.
2006,
Pubmed
Robert,
Patch-clamp-induced perturbations of [Ca(2+)](i) activity in somatotropes.
1999,
Pubmed
Sah,
Ca(2+)-activated K+ currents in neurones: types, physiological roles and modulation.
1996,
Pubmed
Sullivan,
In vivo calcium imaging of circuit activity in cerebellar cortex.
2005,
Pubmed
Takashima,
High-speed CCD imaging system for monitoring neural activity in vivo and in vitro, using a voltage-sensitive dye.
1999,
Pubmed
Wehr,
Odour encoding by temporal sequences of firing in oscillating neural assemblies.
1996,
Pubmed
Yunker,
Immunological characterization of T-type voltage-dependent calcium channel CaV3.1 (alpha 1G) and CaV3.3 (alpha 1I) isoforms reveal differences in their localization, expression, and neural development.
2003,
Pubmed
Zelles,
Branch-specific Ca2+ influx from Na+-dependent dendritic spikes in olfactory granule cells.
2006,
Pubmed
Zufall,
Amplification of odor-induced Ca(2+) transients by store-operated Ca(2+) release and its role in olfactory signal transduction.
2000,
Pubmed
