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EMBO J
1991 Oct 01;1010:2805-11. doi: 10.1002/j.1460-2075.1991.tb07829.x.
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Cloning, expression, pharmacology and regulation of a delayed rectifier K+ channel in mouse heart.
Honoré E
,
Attali B
,
Romey G
,
Heurteaux C
,
Ricard P
,
Lesage F
,
Lazdunski M
,
Barhanin J
.
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Neonatal mouse cardiac poly(A)+ mRNA microinjection into Xenopus oocytes directed the expression of a delayed rectifier K+ current. A cDNA encoding this channel, called mIsK, was cloned from a neonatal mouse heart cDNA library whose properties were studied after expression of the complementary RNA in Xenopus oocytes. Among the different known K+ channel blockers, only the class III antiarrhythmic clofilium inhibited mIsK in the 10-100 microM range. The channel was completely insensitive to other antiarrhythmics such as quinine, quinidine, sotalol or amiodarone. mIsK was enhanced by increasing intracellular Ca2+ and by microinjected Ca(2+)-calmodulin dependent protein kinase II. These stimulations were reversed by the calmodulin antagonist W7. Conversely, the phorbol ester PMA, the diacylglycerol analog OAG and microinjected purified protein kinase C inhibited mIsK. This inhibitory effect could be prevented by the protein kinase C inhibitor staurosporine. These results were consistent with the presence of consensus sequences for kinase II and kinase C in the mIsK structure. Cultured newborn mouse ventricular cardiac cells exhibited a delayed rectifier K+ current which had biophysical properties similar to those of cloned mIsK and which was inhibited by clofilium and protein kinase C activators. In situ hybridization experiments revealed that mIsK mRNA was homogeneously distributed in the cardiac tissue. Neonatal mouse heart expressed the most mIsK mRNA compared with various other rat and mouse tissues. Since this K+ channel generates a current which appears to be involved in the control of both the action potential duration and the beating rate, these results suggest an important role for the mIsK channel in cardiac cell physiology and cardiac pathology.
Arena,
Block of heart potassium channels by clofilium and its tertiary analogs: relationship between drug structure and type of channel blocked.
1988, Pubmed
Arena,
Block of heart potassium channels by clofilium and its tertiary analogs: relationship between drug structure and type of channel blocked.
1988,
Pubmed
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed
Bernardi,
Molecular properties of potassium channels.
1989,
Pubmed
Betz,
Homology and analogy in transmembrane channel design: lessons from synaptic membrane proteins.
1990,
Pubmed
Brown,
Regulation of heartbeat by G protein-coupled ion channels.
1990,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Dascal,
Expression and modulation of voltage-gated calcium channels after RNA injection in Xenopus oocytes.
1986,
Pubmed
,
Xenbase
Folander,
Cloning and expression of the delayed-rectifier IsK channel from neonatal rat heart and diethylstilbestrol-primed rat uterus.
1990,
Pubmed
,
Xenbase
HALL,
Current-voltage relations of Purkinje fibres in sodium-deficient solutions.
1963,
Pubmed
Hamill,
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
1981,
Pubmed
Iwai,
Characterization of gene organization and generation of heterogeneous mRNA species of rat ISK protein.
1990,
Pubmed
Jan,
How might the diversity of potassium channels be generated?
1990,
Pubmed
,
Xenbase
Kameyama,
Intracellular Na+ activates a K+ channel in mammalian cardiac cells.
,
Pubmed
Kemp,
Protein kinase recognition sequence motifs.
1990,
Pubmed
Kim,
Potassium channels in cardiac cells activated by arachidonic acid and phospholipids.
1989,
Pubmed
Kuret,
Purification and characterization of a Ca2+/calmodulin-dependent protein kinase from rat brain.
1984,
Pubmed
McDonald,
The potassium current underlying delayed rectification in cat ventricular muscle.
1978,
Pubmed
Mukherjee,
Differences in myocardial alpha- and beta-adrenergic receptor numbers in different species.
1983,
Pubmed
Murai,
Molecular cloning and sequence analysis of human genomic DNA encoding a novel membrane protein which exhibits a slowly activating potassium channel activity.
1989,
Pubmed
,
Xenbase
Noble,
Outward membrane currents activated in the plateau range of potentials in cardiac Purkinje fibres.
1969,
Pubmed
Noble,
The surprising heart: a review of recent progress in cardiac electrophysiology.
1984,
Pubmed
Noma,
ATP-regulated K+ channels in cardiac muscle.
,
Pubmed
Osterrieder,
Injection of subunits of cyclic AMP-dependent protein kinase into cardiac myocytes modulates Ca2+ current.
1982,
Pubmed
Pragnell,
Estrogen induction of a small, putative K+ channel mRNA in rat uterus.
1990,
Pubmed
,
Xenbase
Reuter,
Ion channels in cardiac cell membranes.
1984,
Pubmed
Roberds,
Cloning and tissue-specific expression of five voltage-gated potassium channel cDNAs expressed in rat heart.
1991,
Pubmed
Romey,
Targets for calcium channel blockers in mammalian skeletal muscle and their respective functions in excitation-contraction coupling.
1988,
Pubmed
Rudy,
Diversity and ubiquity of K channels.
1988,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Sanguinetti,
Two components of cardiac delayed rectifier K+ current. Differential sensitivity to block by class III antiarrhythmic agents.
1990,
Pubmed
Séquier,
Regional expression of a MCD-peptide and dendrotoxin I-sensitive voltage-dependent potassium channel in rat brain.
1990,
Pubmed
Shubeita,
Endothelin induction of inositol phospholipid hydrolysis, sarcomere assembly, and cardiac gene expression in ventricular myocytes. A paracrine mechanism for myocardial cell hypertrophy.
1990,
Pubmed
Siegelbaum,
Calcium-activated transient outward current in calf cardiac Purkinje fibres.
1980,
Pubmed
Stevens,
Ion channels. Making a submicroscopic hole in one.
1991,
Pubmed
Stühmer,
Molecular basis of functional diversity of voltage-gated potassium channels in mammalian brain.
1989,
Pubmed
,
Xenbase
Takumi,
Cloning of a membrane protein that induces a slow voltage-gated potassium current.
1988,
Pubmed
,
Xenbase
Tamkun,
Molecular cloning and characterization of two voltage-gated K+ channel cDNAs from human ventricle.
1991,
Pubmed
Tseng-Crank,
Molecular cloning and functional expression of a potassium channel cDNA isolated from a rat cardiac library.
1990,
Pubmed
,
Xenbase
Walsh,
Regulation of a heart potassium channel by protein kinase A and C.
1988,
Pubmed
Walton,
A three-step purification procedure for protein kinase C: characterization of the purified enzyme.
1987,
Pubmed
Woodgett,
Substrate specificity of protein kinase C. Use of synthetic peptides corresponding to physiological sites as probes for substrate recognition requirements.
1986,
Pubmed
