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Proc Natl Acad Sci U S A
1993 Jun 01;9011:5327-31.
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GAP-43 augments G protein-coupled receptor transduction in Xenopus laevis oocytes.
Strittmatter SM
,
Cannon SC
,
Ross EM
,
Higashijima T
,
Fishman MC
.
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The neuronal protein GAP-43 is thought to play a role in determining growth-cone motility, perhaps as an intracellular regulator of signal transduction, but its molecular mechanism of action has remained unclear. We find that GAP-43, when microinjected into Xenopus laevis oocytes, increases the oocyte response to G protein-coupled receptor agonists by 10- to 100-fold. Higher levels of GAP-43 cause a transient current flow, even without receptor stimulation. The GAP-43-induced current, like receptor-stimulated currents, is mediated by a calcium-activated chloride channel and can be desensitized by injection of inositol 1,4,5-trisphosphate. This suggests that neuronal GAP-43 may serve as an intracellular signal to greatly enhance the sensitivity of G protein-coupled receptor transduction.
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