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XB-ART-7849
Nat Cell Biol 2001 Dec 01;312:1086-91. doi: 10.1038/ncb1201-1086.
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Distinct AAA-ATPase p97 complexes function in discrete steps of nuclear assembly.

Hetzer M , Meyer HH , Walther TC , Bilbao-Cortes D , Warren G , Mattaj IW .


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Although nuclear envelope (NE) assembly is known to require the GTPase Ran, the membrane fusion machinery involved is uncharacterized. NE assembly involves formation of a reticular network on chromatin, fusion of this network into a closed NE and subsequent expansion. Here we show that p97, an AAA-ATPase previously implicated in fusion of Golgi and transitional endoplasmic reticulum (ER) membranes together with the adaptor p47, has two discrete functions in NE assembly. Formation of a closed NE requires the p97-Ufd1-Npl4 complex, not previously implicated in membrane fusion. Subsequent NE growth involves a p97-p47 complex. This study provides the first insights into the molecular mechanisms and specificity of fusion events involved in NE formation.

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Species referenced: Xenopus
Genes referenced: eif4g2 lmnb2 lmnb3 nsfl1c ran ufd1
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References :
Burke, The nuclear envelope: filling in gaps. 2002, Pubmed