Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
The SCL gene specifies haemangioblast development from early mesoderm.
Gering M
,
Rodaway AR
,
Göttgens B
,
Patient RK
,
Green AR
.
???displayArticle.abstract???
The SCL gene encodes a basic helix-loop-helix (bHLH) transcription factor that is essential for the development of all haematopoietic lineages. SCL is also expressed in endothelial cells, but its function is not essential for specification of endothelial progenitors and the role of SCL in endothelial development is obscure. We isolated the zebrafish SCL homologue and show that it was co-expressed in early mesoderm with markers of haematopoietic, endothelial and pronephric progenitors. Ectopic expression of SCL mRNA in zebrafish embryos resulted in overproduction of common haematopoietic and endothelial precursors, perturbation of vasculogenesis and concomitant loss of pronephric duct and somitic tissue. Notochord and neural tube formation were unaffected. These results provide the first evidence that SCL specifies formation of haemangioblasts, the proposed common precursor of blood and endothelial lineages. Our data also underline the striking similarities between the role of SCL in haematopoiesis/vasculogenesis and the function of other bHLH proteins in muscle and neural development.
Altschul,
Basic local alignment search tool.
1990, Pubmed
Altschul,
Basic local alignment search tool.
1990,
Pubmed
Aplan,
The SCL gene product: a positive regulator of erythroid differentiation.
1992,
Pubmed
Badiani,
Dominant interfering alleles define a role for c-Myb in T-cell development.
1994,
Pubmed
Begley,
Chromosomal translocation in a human leukemic stem-cell line disrupts the T-cell antigen receptor delta-chain diversity region and results in a previously unreported fusion transcript.
1989,
Pubmed
Begley,
The gene SCL is expressed during early hematopoiesis and encodes a differentiation-related DNA-binding motif.
1989,
Pubmed
Brand,
asense is a Drosophila neural precursor gene and is capable of initiating sense organ formation.
1993,
Pubmed
Braun,
Targeted inactivation of the muscle regulatory gene Myf-5 results in abnormal rib development and perinatal death.
1992,
Pubmed
Burgess,
Paraxis: a basic helix-loop-helix protein expressed in paraxial mesoderm and developing somites.
1995,
Pubmed
Chen,
The tal gene undergoes chromosome translocation in T cell leukemia and potentially encodes a helix-loop-helix protein.
1990,
Pubmed
Cheng,
Phosphorylation of the TAL1 oncoprotein by the extracellular-signal-regulated protein kinase ERK1.
1993,
Pubmed
Choi,
A common precursor for hematopoietic and endothelial cells.
1998,
Pubmed
Cumano,
Lymphoid potential, probed before circulation in mouse, is restricted to caudal intraembryonic splanchnopleura.
1996,
Pubmed
Cumano,
Differentiation and characterization of B-cell precursors detected in the yolk sac and embryo body of embryos beginning at the 10- to 12-somite stage.
1993,
Pubmed
Davis,
Expression of a single transfected cDNA converts fibroblasts to myoblasts.
1987,
Pubmed
Detrich,
Intraembryonic hematopoietic cell migration during vertebrate development.
1995,
Pubmed
Domínguez,
asense, a member of the Drosophila achaete-scute complex, is a proneural and neural differentiation gene.
1993,
Pubmed
Drake,
TAL1/SCL is expressed in endothelial progenitor cells/angioblasts and defines a dorsal-to-ventral gradient of vasculogenesis.
1997,
Pubmed
Dzierzak,
Mouse embryonic hematopoiesis.
1995,
Pubmed
Eichmann,
Ligand-dependent development of the endothelial and hemopoietic lineages from embryonic mesodermal cells expressing vascular endothelial growth factor receptor 2.
1997,
Pubmed
Ferreiro,
XASH genes promote neurogenesis in Xenopus embryos.
1994,
Pubmed
,
Xenbase
Finger,
Involvement of the TCL5 gene on human chromosome 1 in T-cell leukemia and melanoma.
1989,
Pubmed
Fouquet,
Vessel patterning in the embryo of the zebrafish: guidance by notochord.
1997,
Pubmed
Gavrieli,
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation.
1992,
Pubmed
Godin,
Para-aortic splanchnopleura from early mouse embryos contains B1a cell progenitors.
1993,
Pubmed
Godin,
Emergence of multipotent hemopoietic cells in the yolk sac and paraaortic splanchnopleura in mouse embryos, beginning at 8.5 days postcoitus.
1995,
Pubmed
Green,
Antisense SCL suppresses self-renewal and enhances spontaneous erythroid differentiation of the human leukaemic cell line K562.
1991,
Pubmed
Green,
SCL is coexpressed with GATA-1 in hemopoietic cells but is also expressed in developing brain.
1992,
Pubmed
Griffin,
Analysis of FGF function in normal and no tail zebrafish embryos reveals separate mechanisms for formation of the trunk and the tail.
1995,
Pubmed
Hopwood,
Activation of muscle genes without myogenesis by ectopic expression of MyoD in frog embryo cells.
1990,
Pubmed
,
Xenbase
Hopwood,
Xenopus Myf-5 marks early muscle cells and can activate muscle genes ectopically in early embryos.
1991,
Pubmed
,
Xenbase
Huang,
Identification and characterization of hematopoietic stem cells from the yolk sac of the early mouse embryo.
1993,
Pubmed
Hwang,
Expression of the TAL1 proto-oncogene in cultured endothelial cells and blood vessels of the spleen.
1993,
Pubmed
Jan,
HLH proteins, fly neurogenesis, and vertebrate myogenesis.
1993,
Pubmed
Jowett,
Double fluorescent in situ hybridization to zebrafish embryos.
1996,
Pubmed
Kallianpur,
The SCL/TAL-1 gene is expressed in progenitors of both the hematopoietic and vascular systems during embryogenesis.
1994,
Pubmed
Kennedy,
A common precursor for primitive erythropoiesis and definitive haematopoiesis.
1997,
Pubmed
Krauss,
Expression pattern of zebrafish pax genes suggests a role in early brain regionalization.
1991,
Pubmed
Krieg,
Functional messenger RNAs are produced by SP6 in vitro transcription of cloned cDNAs.
1984,
Pubmed
,
Xenbase
Lee,
Conversion of Xenopus ectoderm into neurons by NeuroD, a basic helix-loop-helix protein.
1995,
Pubmed
,
Xenbase
Leroy-Viard,
Loss of TAL-1 protein activity induces premature apoptosis of Jurkat leukemic T cells upon medium depletion.
1995,
Pubmed
Liao,
SCL/Tal-1 transcription factor acts downstream of cloche to specify hematopoietic and vascular progenitors in zebrafish.
1998,
Pubmed
Liao,
The zebrafish gene cloche acts upstream of a flk-1 homologue to regulate endothelial cell differentiation.
1997,
Pubmed
Liu,
In vitro development of murine T cells from prethymic and preliver embryonic yolk sac hematopoietic stem cells.
1991,
Pubmed
Martínez,
Cross-regulatory interactions between the proneural achaete and scute genes of Drosophila.
1991,
Pubmed
Medvinsky,
Definitive hematopoiesis is autonomously initiated by the AGM region.
1996,
Pubmed
Medvinsky,
An early pre-liver intraembryonic source of CFU-S in the developing mouse.
1993,
Pubmed
Moore,
Ontogeny of the haemopoietic system: yolk sac origin of in vivo and in vitro colony forming cells in the developing mouse embryo.
1970,
Pubmed
Naya,
Diabetes, defective pancreatic morphogenesis, and abnormal enteroendocrine differentiation in BETA2/neuroD-deficient mice.
1997,
Pubmed
Neave,
A graded response to BMP-4 spatially coordinates patterning of the mesoderm and ectoderm in the zebrafish.
1997,
Pubmed
Nishikawa,
Progressive lineage analysis by cell sorting and culture identifies FLK1+VE-cadherin+ cells at a diverging point of endothelial and hemopoietic lineages.
1998,
Pubmed
Porcher,
The T cell leukemia oncoprotein SCL/tal-1 is essential for development of all hematopoietic lineages.
1996,
Pubmed
Prasad,
Target-dependent effect of phosphorylation on the DNA binding activity of the TAL1/SCL oncoprotein.
1997,
Pubmed
Robb,
Absence of yolk sac hematopoiesis from mice with a targeted disruption of the scl gene.
1995,
Pubmed
Robb,
The scl gene product is required for the generation of all hematopoietic lineages in the adult mouse.
1996,
Pubmed
Rodríguez,
Competence to develop sensory organs is temporally and spatially regulated in Drosophila epidermal primordia.
1990,
Pubmed
Rudnicki,
MyoD or Myf-5 is required for the formation of skeletal muscle.
1993,
Pubmed
Rudnicki,
Inactivation of MyoD in mice leads to up-regulation of the myogenic HLH gene Myf-5 and results in apparently normal muscle development.
1992,
Pubmed
Shalaby,
A requirement for Flk1 in primitive and definitive hematopoiesis and vasculogenesis.
1997,
Pubmed
Shalaby,
Failure of blood-island formation and vasculogenesis in Flk-1-deficient mice.
1995,
Pubmed
Shivdasani,
Absence of blood formation in mice lacking the T-cell leukaemia oncoprotein tal-1/SCL.
1995,
Pubmed
Silver,
Initiation of murine embryonic erythropoiesis: a spatial analysis.
1997,
Pubmed
Sumoy,
A role for notochord in axial vascular development revealed by analysis of phenotype and the expression of VEGR-2 in zebrafish flh and ntl mutant embryos.
1997,
Pubmed
Tavian,
Aorta-associated CD34+ hematopoietic cells in the early human embryo.
1996,
Pubmed
Thompson,
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
1997,
Pubmed
Turner,
Expression of achaete-scute homolog 3 in Xenopus embryos converts ectodermal cells to a neural fate.
1994,
Pubmed
,
Xenbase
Turpen,
Bipotential primitive-definitive hematopoietic progenitors in the vertebrate embryo.
1997,
Pubmed
,
Xenbase
Visvader,
Unsuspected role for the T-cell leukemia protein SCL/tal-1 in vascular development.
1998,
Pubmed
Weinberg,
Developmental regulation of zebrafish MyoD in wild-type, no tail and spadetail embryos.
1996,
Pubmed
Weinstein,
Hematopoietic mutations in the zebrafish.
1996,
Pubmed
Weintraub,
The MyoD family and myogenesis: redundancy, networks, and thresholds.
1993,
Pubmed
Wong,
Properties of the earliest clonogenic hemopoietic precursors to appear in the developing murine yolk sac.
1986,
Pubmed
Wood,
CD34 expression patterns during early mouse development are related to modes of blood vessel formation and reveal additional sites of hematopoiesis.
1997,
Pubmed
Wülbeck,
The HLH domain of a zebrafish HE12 homologue can partially substitute for functions of the HLH domain of Drosophila DAUGHTERLESS.
1994,
Pubmed
Zernicka-Goetz,
An indelible lineage marker for Xenopus using a mutated green fluorescent protein.
1996,
Pubmed
,
Xenbase
Zon,
Developmental biology of hematopoiesis.
1995,
Pubmed