Göttgens B , Nastos A , Kinston S , Piltz S , Delabesse EC , Stanley M , Sanchez MJ , Ciau-Uitz A , Patient R , Green AR .

Fig. 4. The +19 core enhancer is active in transgenic frogs and murine haematopoietic progenitor cells and critically depends on regions 1, 2 and 3. (A) An example of the variable ectopic expression patterns seen in a minority of transgenic stage 32 embryos with the SV/GFP construct (ectopic expression was never seen in the DLP region). (B) SV/GFP/H2.5 transgenic embryo showing reporter gene expression in the DLP region (arrow). (C–E) Stage 27 SV/GFP/H2.5 transgenic embryo stained for GFP (BCIP, turquoise) and endogenous SCL (BM Purple). d/e indicates the plane of the section shown in (D) and (E) which demonstrate that the purple staining (endogenous SCL) is contained within the area of transgene expression (turquoise) in the DLP (arrow). (F) Summary of transgenic frog experiments using wild-type and mutant versions of the SH0.6 core enhancer. DLP, embryos showing DLP staining with or without ectopic expression; ectopic, embryos showing ectopic expression only. The number in each bar represents the number of GFP-expressing embryos for each category. (G) The SV/luc/SH0.6 construct was active in 416B but not BW5147 cells in stable transfection assays. (H) Stable transfection assays in 416B cells demonstrate that the SH0.6 enhancer activity critically depends on the 227 bp 3′ fragment deleted in the SA0.4 construct, and that the three conserved regions mutated in the mut1, mut2 and mut3 constructs are each critical for enhancer function.

Akashi, A clonogenic common myeloid progenitor that gives rise to all myeloid lineages. 2000, Pubmed

Akashi, A clonogenic common myeloid progenitor that gives rise to all myeloid lineages. 2000, Pubmed
Aplan, The SCL gene is formed from a transcriptionally complex locus. 1990, Pubmed
Baltzinger, Xl erg: expression pattern and overexpression during development plead for a role in endothelial cell differentiation. 1999, Pubmed , Xenbase
Bartel, Mouse models in the study of the Ets family of transcription factors. 2000, Pubmed
Begley, Structure of the gene encoding the murine SCL protein. 1994, Pubmed
Begley, The SCL gene: from case report to critical hematopoietic regulator. 1999, Pubmed
Bertwistle, GATA factors and the origins of adult and embryonic blood in Xenopus: responses to retinoic acid. 1996, Pubmed , Xenbase
Blau, The evolving concept of a stem cell: entity or function? 2001, Pubmed
Blobel, CREB-binding protein cooperates with transcription factor GATA-1 and is required for erythroid differentiation. 1998, Pubmed
Bockamp, Distinct mechanisms direct SCL/tal-1 expression in erythroid cells and CD34 positive primitive myeloid cells. 1997, Pubmed
Bockamp, Lineage-restricted regulation of the murine SCL/TAL-1 promoter. 1995, Pubmed
Bockamp, Transcriptional regulation of the stem cell leukemia gene by PU.1 and Elf-1. 1998, Pubmed
Boyes, Tissue-specific factors additively increase the probability of the all-or-none formation of a hypersensitive site. 1996, Pubmed
Boyes, Perturbation of nucleosome structure by the erythroid transcription factor GATA-1. 1998, Pubmed
Briegel, Ectopic expression of a conditional GATA-2/estrogen receptor chimera arrests erythroid differentiation in a hormone-dependent manner. 1993, Pubmed
Brown, Insights into early vasculogenesis revealed by expression of the ETS-domain transcription factor Fli-1 in wild-type and mutant zebrafish embryos. 2000, Pubmed
Carey, The enhanceosome and transcriptional synergy. 1998, Pubmed
Choi, A common precursor for hematopoietic and endothelial cells. 1998, Pubmed
Ciau-Uitz, Distinct origins of adult and embryonic blood in Xenopus. 2000, Pubmed , Xenbase
Cross, Expression of lineage restricted transcription factors precedes lineage specific differentiation in a multipotent haemopoietic progenitor cell line. 1994, Pubmed
Cumano, Pluripotent hematopoietic stem cell development during embryogenesis. 2001, Pubmed
Dai, p300 Functions as a coactivator of transcription factor GATA-4. 2001, Pubmed
Davis, Cloning and expression of the murine Elf-1 cDNA. 1996, Pubmed
de Bruijn, Definitive hematopoietic stem cells first develop within the major arterial regions of the mouse embryo. 2000, Pubmed
Dieterlen-Lievre, On the origin of haemopoietic stem cells in the avian embryo: an experimental approach. 1975, Pubmed
Drake, TAL1/SCL is expressed in endothelial progenitor cells/angioblasts and defines a dorsal-to-ventral gradient of vasculogenesis. 1997, Pubmed
Dube, Elf-1 is a transcriptional regulator of the Tie2 gene during vascular development. 2001, Pubmed
Dzierzak, Mouse embryonic hematopoiesis. 1995, Pubmed
Eichmann, Ligand-dependent development of the endothelial and hemopoietic lineages from embryonic mesodermal cells expressing vascular endothelial growth factor receptor 2. 1997, Pubmed
Ellis, A dominant chromatin-opening activity in 5' hypersensitive site 3 of the human beta-globin locus control region. 1996, Pubmed
Forsberg, Direct interaction of NF-E2 with hypersensitive site 2 of the beta-globin locus control region in living cells. 2000, Pubmed
Fuchs, Stem cells: a new lease on life. 2000, Pubmed
Garcia-Porrero, Potential intraembryonic hemogenic sites at pre-liver stages in the mouse. 1995, Pubmed
Garrett-Sinha, PU.1 exhibits partial functional redundancy with Spi-B, but not with Ets-1 or Elf-1. 2001, Pubmed
Gering, The SCL gene specifies haemangioblast development from early mesoderm. 1998, Pubmed , Xenbase
Giese, Assembly and function of a TCR alpha enhancer complex is dependent on LEF-1-induced DNA bending and multiple protein-protein interactions. 1995, Pubmed
Göttgens, Transcription of the SCL gene in erythroid and CD34 positive primitive myeloid cells is controlled by a complex network of lineage-restricted chromatin-dependent and chromatin-independent regulatory elements. 1997, Pubmed
Göttgens, Long-range comparison of human and mouse SCL loci: localized regions of sensitivity to restriction endonucleases correspond precisely with peaks of conserved noncoding sequences. 2001, Pubmed
Göttgens, Analysis of vertebrate SCL loci identifies conserved enhancers. 2000, Pubmed , Xenbase
Green, SCL is coexpressed with GATA-1 in hemopoietic cells but is also expressed in developing brain. 1992, Pubmed
Green, Antisense SCL suppresses self-renewal and enhances spontaneous erythroid differentiation of the human leukaemic cell line K562. 1991, Pubmed
Hart, Fli-1 is required for murine vascular and megakaryocytic development and is hemizygously deleted in patients with thrombocytopenia. 2000, Pubmed
Jaffredo, Intraaortic hemopoietic cells are derived from endothelial cells during ontogeny. 1998, Pubmed
Jaffredo, Tracing the progeny of the aortic hemangioblast in the avian embryo. 2000, Pubmed
Jeong, Micrococcal nuclease digestion of nuclei reveals extended nucleosome ladders having anomalous DNA lengths for chromatin assembled on non-replicating plasmids in transfected cells. 1994, Pubmed
John, Regulation of cell-type-specific interleukin-2 receptor alpha-chain gene expression: potential role of physical interactions between Elf-1, HMG-I(Y), and NF-kappa B family proteins. 1995, Pubmed
Kallianpur, The SCL/TAL-1 gene is expressed in progenitors of both the hematopoietic and vascular systems during embryogenesis. 1994, Pubmed
Kerr, Association between proto-oncoprotein Rel and TATA-binding protein mediates transcriptional activation by NF-kappa B. 1993, Pubmed
Kim, The mechanism of transcriptional synergy of an in vitro assembled interferon-beta enhanceosome. 1997, Pubmed
Kroll, Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation. 1996, Pubmed , Xenbase
Krosl, Transcription factor SCL is required for c-kit expression and c-Kit function in hemopoietic cells. 1998, Pubmed
Lecointe, GATA-and SP1-binding sites are required for the full activity of the tissue-specific promoter of the tal-1 gene. 1994, Pubmed
Lee, Cloning of the GATA-binding protein that regulates endothelin-1 gene expression in endothelial cells. 1991, Pubmed
Li, Locus control regions: coming of age at a decade plus. 1999, Pubmed
Li, Regulation of the PU.1 gene by distal elements. 2001, Pubmed
Liang, The transcription factor GATA4 is activated by extracellular signal-regulated kinase 1- and 2-mediated phosphorylation of serine 105 in cardiomyocytes. 2001, Pubmed
Liao, The zebrafish gene cloche acts upstream of a flk-1 homologue to regulate endothelial cell differentiation. 1997, Pubmed
Liao, SCL/Tal-1 transcription factor acts downstream of cloche to specify hematopoietic and vascular progenitors in zebrafish. 1998, Pubmed
Lovell-Badge, The future for stem cell research. 2001, Pubmed
Mager, The avian fli gene is specifically expressed during embryogenesis in a subset of neural crest cells giving rise to mesenchyme. 1998, Pubmed , Xenbase
May, Targeting gene expression to haemopoietic stem cells: a chromatin-dependent upstream element mediates cell type-specific expression of the stem cell antigen CD34. 1995, Pubmed
Mead, SCL specifies hematopoietic mesoderm in Xenopus embryos. 1998, Pubmed , Xenbase
Mélet, Generation of a novel Fli-1 protein by gene targeting leads to a defect in thymus development and a delay in Friend virus-induced erythroleukemia. 1996, Pubmed
Merika, Enhanceosomes. 2001, Pubmed
Metcalf, Regulatory mechanisms controlling hematopoiesis: principles and problems. 1998, Pubmed
Meyer, Whole-mount in situ hybridization reveals the expression of the Xl-Fli gene in several lineages of migrating cells in Xenopus embryos. 1995, Pubmed , Xenbase
Minegishi, The mouse GATA-2 gene is expressed in the para-aortic splanchnopleura and aorta-gonads and mesonephros region. 1999, Pubmed
Miyazaki, MEF, a novel transcription factor with an Elf-1 like DNA binding domain but distinct transcriptional activating properties. 1996, Pubmed
Morrison, Stem cell potential: can anything make anything? 2001, Pubmed
Mouthon, Expression of tal-1 and GATA-binding proteins during human hematopoiesis. 1993, Pubmed
Mukouyama, The AML1 transcription factor functions to develop and maintain hematogenic precursor cells in the embryonic aorta-gonad-mesonephros region. 2000, Pubmed
Muro-Pastor, The GATA factor AreA is essential for chromatin remodelling in a eukaryotic bidirectional promoter. 1999, Pubmed
Nishikawa, In vitro generation of lymphohematopoietic cells from endothelial cells purified from murine embryos. 1998, Pubmed
Nishikawa, Progressive lineage analysis by cell sorting and culture identifies FLK1+VE-cadherin+ cells at a diverging point of endothelial and hemopoietic lineages. 1998, Pubmed
North, Cbfa2 is required for the formation of intra-aortic hematopoietic clusters. 1999, Pubmed
Okuda, AML1, the target of multiple chromosomal translocations in human leukemia, is essential for normal fetal liver hematopoiesis. 1996, Pubmed
Orkin, Diversification of haematopoietic stem cells to specific lineages. 2000, Pubmed
Palis, Yolk-sac hematopoiesis: the first blood cells of mouse and man. 2001, Pubmed , Xenbase
Péault, Hematopoietic stem cell emergence in embryonic life: developmental hematology revisited. 1996, Pubmed
Porcher, The T cell leukemia oncoprotein SCL/tal-1 is essential for development of all hematopoietic lineages. 1996, Pubmed
Robb, The scl gene product is required for the generation of all hematopoietic lineages in the adult mouse. 1996, Pubmed
Robertson, A transitional stage in the commitment of mesoderm to hematopoiesis requiring the transcription factor SCL/tal-1. 2000, Pubmed
Sánchez, An SCL 3' enhancer targets developing endothelium together with embryonic and adult haematopoietic progenitors. 1999, Pubmed
Sánchez, Selective rescue of early haematopoietic progenitors in Scl(-/-) mice by expressing Scl under the control of a stem cell enhancer. 2001, Pubmed
Sinclair, Distinct 5' SCL enhancers direct transcription to developing brain, spinal cord, and endothelium: neural expression is mediated by GATA factor binding sites. 1999, Pubmed
Sparrow, A simplified method of generating transgenic Xenopus. 2000, Pubmed , Xenbase
Spyropoulos, Hemorrhage, impaired hematopoiesis, and lethality in mouse embryos carrying a targeted disruption of the Fli1 transcription factor. 2000, Pubmed
Stainier, Cloche, an early acting zebrafish gene, is required by both the endothelial and hematopoietic lineages. 1995, Pubmed
Takakura, A role for hematopoietic stem cells in promoting angiogenesis. 2000, Pubmed
Tapscott, A novel myoblast enhancer element mediates MyoD transcription. 1992, Pubmed
Towatari, Regulation of GATA-2 phosphorylation by mitogen-activated protein kinase and interleukin-3. 1995, Pubmed
Truong, The role of Fli-1 in normal cell function and malignant transformation. 2000, Pubmed
Tsai, Lymphohematopoietic progenitors immortalized by a retroviral vector harboring a dominant-negative retinoic acid receptor can recapitulate lymphoid, myeloid, and erythroid development. 1994, Pubmed
Tsai, An early haematopoietic defect in mice lacking the transcription factor GATA-2. 1994, Pubmed
Visvader, Unsuspected role for the T-cell leukemia protein SCL/tal-1 in vascular development. 1998, Pubmed
Vlaeminck-Guillem, The Ets family member Erg gene is expressed in mesodermal tissues and neural crests at fundamental steps during mouse embryogenesis. 2000, Pubmed
von Bubnoff, Intracellular BMP signaling regulation in vertebrates: pathway or network? 2001, Pubmed , Xenbase
Wada, A p300 protein as a coactivator of GATA-6 in the transcription of the smooth muscle-myosin heavy chain gene. 2000, Pubmed
Wang, Disruption of the Cbfa2 gene causes necrosis and hemorrhaging in the central nervous system and blocks definitive hematopoiesis. 1996, Pubmed
Warren, The oncogenic cysteine-rich LIM domain protein rbtn2 is essential for erythroid development. 1994, Pubmed
Weiss, Novel insights into erythroid development revealed through in vitro differentiation of GATA-1 embryonic stem cells. 1994, Pubmed
Weissman, Stem cells: units of development, units of regeneration, and units in evolution. 2000, Pubmed
Yordy, Signal transduction and the Ets family of transcription factors. 2000, Pubmed