Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
1998 Jun 01;1711:3168-75. doi: 10.1093/emboj/17.11.3168.
Show Gene links
Show Anatomy links
Meiotic maturation in Xenopus requires polyadenylation of multiple mRNAs.
Barkoff A
,
Ballantyne S
,
Wickens M
.
???displayArticle.abstract???
Cytoplasmic polyadenylation of specific mRNAs commonly is correlated with their translational activation during development. Here, we focus on links between cytoplasmic polyadenylation, translational activation and the control of meiotic maturation in Xenopus oocytes. We manipulate endogenous c-mos mRNA, which encodes a protein kinase that regulates meiotic maturation. We determined that translational activation of endogenous c-mos mRNA requires a long poly(A) tail per se, rather than the process of polyadenylation. For this, we injected 'prosthetic' poly(A)_synthetic poly(A) tails designed to attach by base pairing to endogenous c-mos mRNA that has had its own polyadenylation signals removed. This prosthetic poly(A) tail activates c-mos translation and restores meiotic maturation in response to progesterone. Thus the role of polyadenylation in activating c-mos mRNA differs from its role in activating certain other mRNAs, for which the act of polyadenylation is required. In the absence of progesterone, prosthetic poly(A) does not stimulate c-mos expression, implying that progesterone acts at additional steps to elevate c-Mos protein. By using a general inhibitor of polyadenylation together with prosthetic poly(A), we demonstrate that these additional steps include polyadenylation of at least one other mRNA, in addition to that of c-mos mRNA. These other mRNAs, encoding regulators of meiotic maturation, act upstream of c-Mos in the meiotic maturation pathway.
Ballantyne,
A dependent pathway of cytoplasmic polyadenylation reactions linked to cell cycle control by c-mos and CDK1 activation.
1997, Pubmed,
Xenbase
Ballantyne,
A dependent pathway of cytoplasmic polyadenylation reactions linked to cell cycle control by c-mos and CDK1 activation.
1997,
Pubmed
,
Xenbase
Ballantyne,
Poly (A) polymerases in the nucleus and cytoplasm of frog oocytes: dynamic changes during oocyte maturation and early development.
1995,
Pubmed
,
Xenbase
Bouvet,
A role for transcription and FRGY2 in masking maternal mRNA within Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Braddock,
Intron-less RNA injected into the nucleus of Xenopus oocytes accesses a regulated translation control pathway.
1994,
Pubmed
,
Xenbase
Colledge,
Disruption of c-mos causes parthenogenetic development of unfertilized mouse eggs.
1994,
Pubmed
,
Xenbase
Curtis,
Translational regulation in development.
1995,
Pubmed
Darnell,
Polyadenylic acid sequences: role in conversion of nuclear RNA into messenger RNA.
1971,
Pubmed
Fox,
Poly(A) removal during oocyte maturation: a default reaction selectively prevented by specific sequences in the 3' UTR of certain maternal mRNAs.
1990,
Pubmed
,
Xenbase
Fox,
Poly(A) addition during maturation of frog oocytes: distinct nuclear and cytoplasmic activities and regulation by the sequence UUUUUAU.
1989,
Pubmed
,
Xenbase
Galili,
Role of the 3'-poly(A) sequence in translational regulation of mRNAs in Xenopus laevis oocytes.
1988,
Pubmed
,
Xenbase
Gebauer,
Translational control by cytoplasmic polyadenylation of c-mos mRNA is necessary for oocyte maturation in the mouse.
1994,
Pubmed
Gotoh,
Initiation of Xenopus oocyte maturation by activation of the mitogen-activated protein kinase cascade.
1995,
Pubmed
,
Xenbase
Hashimoto,
Parthenogenetic activation of oocytes in c-mos-deficient mice.
1994,
Pubmed
,
Xenbase
Kuge,
Cytoplasmic 3' poly(A) addition induces 5' cap ribose methylation: implications for translational control of maternal mRNA.
1995,
Pubmed
,
Xenbase
Kuge,
Maturation of Xenopus laevis oocyte by progesterone requires poly(A) tail elongation of mRNA.
1992,
Pubmed
,
Xenbase
Lieberfarb,
Mutations that perturb poly(A)-dependent maternal mRNA activation block the initiation of development.
1996,
Pubmed
Maale,
Effects of cordycepin and cordycepintriphosphate on polyadenylic and ribonucleic acid-synthesising enzymes from eukaryotes.
1975,
Pubmed
McGrew,
Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element.
1989,
Pubmed
,
Xenbase
Meric,
Regulated unmasking of in vivo synthesized maternal mRNA at oocyte maturation. A role for the chaperone nucleoplasmin.
1997,
Pubmed
,
Xenbase
Moore,
Analysis of RNA cleavage at the adenovirus-2 L3 polyadenylation site.
1986,
Pubmed
Nebreda,
Newly synthesized protein(s) must associate with p34cdc2 to activate MAP kinase and MPF during progesterone-induced maturation of Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Nishizawa,
The 'second-codon rule' and autophosphorylation govern the stability and activity of Mos during the meiotic cell cycle in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Paris,
Maturation-specific polyadenylation and translational control: diversity of cytoplasmic polyadenylation elements, influence of poly(A) tail size, and formation of stable polyadenylation complexes.
1990,
Pubmed
,
Xenbase
Sachs,
Purification and characterization of polyadenylate-binding protein.
1990,
Pubmed
Sagata,
The product of the mos proto-oncogene as a candidate "initiator" for oocyte maturation.
1989,
Pubmed
,
Xenbase
Sagata,
Function of c-mos proto-oncogene product in meiotic maturation in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Sagata,
What does Mos do in oocytes and somatic cells?
1997,
Pubmed
,
Xenbase
Sallés,
Coordinate initiation of Drosophila development by regulated polyadenylation of maternal messenger RNAs.
1994,
Pubmed
Sheets,
Polyadenylation of c-mos mRNA as a control point in Xenopus meiotic maturation.
1995,
Pubmed
,
Xenbase
Sheets,
The 3'-untranslated regions of c-mos and cyclin mRNAs stimulate translation by regulating cytoplasmic polyadenylation.
1994,
Pubmed
,
Xenbase
Sheets,
Products of in vitro cleavage and polyadenylation of simian virus 40 late pre-mRNAs.
1987,
Pubmed
Simon,
Translational control by poly(A) elongation during Xenopus development: differential repression and enhancement by a novel cytoplasmic polyadenylation element.
1992,
Pubmed
,
Xenbase
Sippel,
Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransferase from Escherichia coli.
1973,
Pubmed
Varnum,
Deadenylation of maternal mRNAs during Xenopus oocyte maturation does not require specific cis-sequences: a default mechanism for translational control.
1990,
Pubmed
,
Xenbase
Vassalli,
Regulated polyadenylation controls mRNA translation during meiotic maturation of mouse oocytes.
1989,
Pubmed
Verrotti,
Evolutionary conservation of sequence elements controlling cytoplasmic polyadenylylation.
1996,
Pubmed
,
Xenbase
Wickens,
Life and death in the cytoplasm: messages from the 3' end.
1997,
Pubmed
Wreden,
Nanos and pumilio establish embryonic polarity in Drosophila by promoting posterior deadenylation of hunchback mRNA.
1997,
Pubmed
Yew,
Meiotic initiation by the mos protein in Xenopus.
1992,
Pubmed
,
Xenbase
