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Extreme nuclear branching in healthy epidermal cells of the Xenopus tailfin.
Arbach HE
,
Harland-Dunaway M
,
Chang JK
,
Wills AE
.
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Changes in nuclear morphology contribute to the regulation of complex cell properties, including differentiation and tissue elasticity. Perturbations of nuclear morphology are associated with pathologies that include progeria, cancer and muscular dystrophy. The mechanisms governing nuclear shape changes in healthy cells remain poorly understood, partially because there are few models of nuclear shape variation in healthy cells. Here, we introduce nuclear branching in epidermal fin cells of Xenopus tropicalis as a model for extreme variation of nuclear morphology in a diverse population of healthy cells. We found that nuclear branching arises within these cells and becomes more elaborate during embryonic development. These cells contain broadly distributed marks of transcriptionally active chromatin and heterochromatin, and have active cell cycles. We found that nuclear branches are disrupted by loss of filamentous actin and depend on epidermal expression of the nuclear lamina protein Lamin B1. Inhibition of nuclear branching disrupts fin morphology, suggesting that nuclear branching may be involved in fin development. This study introduces the nuclei of the Xenopus fin as a powerful new model for extreme nuclear morphology in healthy cells to complement studies of nuclear shape variation in pathological contexts.This article has an associated First Person interview with the first author of the paper.
Baarlink,
A transient pool of nuclear F-actin at mitotic exit controls chromatin organization.
2017, Pubmed
Baarlink,
A transient pool of nuclear F-actin at mitotic exit controls chromatin organization.
2017,
Pubmed
Bauer,
The cleavage stage origin of Spemann's Organizer: analysis of the movements of blastomere clones before and during gastrulation in Xenopus.
1994,
Pubmed
,
Xenbase
Bhattacharya,
CRISPR/Cas9: An inexpensive, efficient loss of function tool to screen human disease genes in Xenopus.
2015,
Pubmed
,
Xenbase
Bonne,
Mutations in the gene encoding lamin A/C cause autosomal dominant Emery-Dreifuss muscular dystrophy.
1999,
Pubmed
Brangwynne,
Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes.
2011,
Pubmed
,
Xenbase
Buntrock,
Organ growth without cell division: somatic polyploidy in a moth, Ephestia kuehniella.
2012,
Pubmed
Chang,
Accessorizing and anchoring the LINC complex for multifunctionality.
2015,
Pubmed
Chen,
Dysregulated interactions between lamin A and SUN1 induce abnormalities in the nuclear envelope and endoplasmic reticulum in progeric laminopathies.
2014,
Pubmed
Cheutin,
Maintenance of stable heterochromatin domains by dynamic HP1 binding.
2003,
Pubmed
Dahl,
Distinct structural and mechanical properties of the nuclear lamina in Hutchinson-Gilford progeria syndrome.
2006,
Pubmed
Davidson,
Broken nuclei--lamins, nuclear mechanics, and disease.
2014,
Pubmed
Davis,
The fate of cells in the tailbud of Xenopus laevis.
2000,
Pubmed
,
Xenbase
Denais,
Nuclear mechanics in cancer.
2014,
Pubmed
Dreesen,
Lamin B1 fluctuations have differential effects on cellular proliferation and senescence.
2013,
Pubmed
Dutta,
Turnover of the actomyosin complex in zebrafish embryos directs geometric remodelling and the recruitment of lipid droplets.
2015,
Pubmed
Fraser,
Hierarchical folding and reorganization of chromosomes are linked to transcriptional changes in cellular differentiation.
2015,
Pubmed
Frost,
Lamin Dysfunction Mediates Neurodegeneration in Tauopathies.
2016,
Pubmed
Fu,
Nuclear deformation during breast cancer cell transmigration.
2012,
Pubmed
Gdula,
Remodeling of three-dimensional organization of the nucleus during terminal keratinocyte differentiation in the epidermis.
2013,
Pubmed
Giarmarco,
Mitochondria Maintain Distinct Ca2+ Pools in Cone Photoreceptors.
2017,
Pubmed
Goldman,
Accumulation of mutant lamin A causes progressive changes in nuclear architecture in Hutchinson-Gilford progeria syndrome.
2004,
Pubmed
Good,
Cytoplasmic volume modulates spindle size during embryogenesis.
2013,
Pubmed
,
Xenbase
Guilak,
Viscoelastic properties of the cell nucleus.
2000,
Pubmed
Hatch,
Catastrophic nuclear envelope collapse in cancer cell micronuclei.
2013,
Pubmed
Hatch,
Nuclear envelope rupture is induced by actin-based nucleus confinement.
2016,
Pubmed
Ho,
Lamin A/C and emerin regulate MKL1-SRF activity by modulating actin dynamics.
2013,
Pubmed
Jevtić,
Concentration-dependent Effects of Nuclear Lamins on Nuclear Size in Xenopus and Mammalian Cells.
2015,
Pubmed
,
Xenbase
Jevtić,
Nuclear size scaling during Xenopus early development contributes to midblastula transition timing.
2015,
Pubmed
,
Xenbase
Kalendová,
Nuclear actin filaments recruit cofilin and actin-related protein 3, and their formation is connected with a mitotic block.
2014,
Pubmed
Keeling,
Actomyosin and vimentin cytoskeletal networks regulate nuclear shape, mechanics and chromatin organization.
2017,
Pubmed
Kha,
Stiffness properties for Nucleus standard straight and contour electrode arrays.
2004,
Pubmed
Khokha,
Techniques and probes for the study of Xenopus tropicalis development.
2002,
Pubmed
,
Xenbase
Kieserman,
High-magnification in vivo imaging of Xenopus embryos for cell and developmental biology.
2010,
Pubmed
,
Xenbase
Kim,
Cytoskeletal tension induces the polarized architecture of the nucleus.
2015,
Pubmed
King,
A model for coordinating nuclear mechanics and membrane remodeling to support nuclear integrity.
2016,
Pubmed
Lammerding,
Lamins A and C but not lamin B1 regulate nuclear mechanics.
2006,
Pubmed
Lee,
Actomyosin contractility and microtubules drive apical constriction in Xenopus bottle cells.
2007,
Pubmed
,
Xenbase
Lemaitre,
Dynamics of the genome during early Xenopus laevis development: karyomeres as independent units of replication.
1998,
Pubmed
,
Xenbase
Levy,
Nuclear size is regulated by importin α and Ntf2 in Xenopus.
2010,
Pubmed
,
Xenbase
Li,
Lamin Mutations Accelerate Aging via Defective Export of Mitochondrial mRNAs through Nuclear Envelope Budding.
2016,
Pubmed
Mattout,
Chromatin states and nuclear organization in development--a view from the nuclear lamina.
2015,
Pubmed
Mattout,
Heterochromatin Protein 1β (HP1β) has distinct functions and distinct nuclear distribution in pluripotent versus differentiated cells.
2015,
Pubmed
McKenna,
Embryonic expression of the common progeroid lamin A splice mutation arrests postnatal skin development.
2014,
Pubmed
Moody,
Fates of the blastomeres of the 16-cell stage Xenopus embryo.
1987,
Pubmed
,
Xenbase
Nakayama,
Simple and efficient CRISPR/Cas9-mediated targeted mutagenesis in Xenopus tropicalis.
2013,
Pubmed
,
Xenbase
Oda,
Chromatin tethering to the nuclear envelope by nuclear actin filaments: a novel role of the actin cytoskeleton in the Xenopus blastula.
2017,
Pubmed
,
Xenbase
Pajerowski,
Physical plasticity of the nucleus in stem cell differentiation.
2007,
Pubmed
Peric-Hupkes,
Molecular maps of the reorganization of genome-nuclear lamina interactions during differentiation.
2010,
Pubmed
Perovanovic,
Laminopathies disrupt epigenomic developmental programs and cell fate.
2016,
Pubmed
Pillay,
Immune suppression by neutrophils and granulocytic myeloid-derived suppressor cells: similarities and differences.
2013,
Pubmed
Plessner,
Nuclear F-actin formation and reorganization upon cell spreading.
2015,
Pubmed
Ramdas,
Cytoskeletal control of nuclear morphology and chromatin organization.
2015,
Pubmed
Rowat,
Nuclear envelope composition determines the ability of neutrophil-type cells to passage through micron-scale constrictions.
2013,
Pubmed
Schirmer,
Involvement of the lamin rod domain in heterotypic lamin interactions important for nuclear organization.
2001,
Pubmed
Schöchlin,
A nuclear circularity-based classifier for diagnostic distinction of desmoplastic from spindle cell melanoma in digitized histological images.
2014,
Pubmed
Schoft,
The lamina-associated polypeptide 2 (LAP2) isoforms beta, gamma and omega of zebrafish: developmental expression and behavior during the cell cycle.
2003,
Pubmed
,
Xenbase
Sedzinski,
Emergence of an Apical Epithelial Cell Surface In Vivo.
2016,
Pubmed
,
Xenbase
Session,
Genome evolution in the allotetraploid frog Xenopus laevis.
2016,
Pubmed
,
Xenbase
Shah,
Lamin B1 depletion in senescent cells triggers large-scale changes in gene expression and the chromatin landscape.
2013,
Pubmed
Solovei,
LBR and lamin A/C sequentially tether peripheral heterochromatin and inversely regulate differentiation.
2013,
Pubmed
Swift,
Nuclear lamin-A scales with tissue stiffness and enhances matrix-directed differentiation.
2013,
Pubmed
Tariq,
Lamin A and microtubules collaborate to maintain nuclear morphology.
2017,
Pubmed
Towbin,
Step-wise methylation of histone H3K9 positions heterochromatin at the nuclear periphery.
2012,
Pubmed
Tucker,
Independent induction and formation of the dorsal and ventral fins in Xenopus laevis.
2004,
Pubmed
,
Xenbase
Vergnes,
Lamin B1 is required for mouse development and nuclear integrity.
2004,
Pubmed
Versaevel,
Spatial coordination between cell and nuclear shape within micropatterned endothelial cells.
2012,
Pubmed
Verstraeten,
Increased mechanosensitivity and nuclear stiffness in Hutchinson-Gilford progeria cells: effects of farnesyltransferase inhibitors.
2008,
Pubmed
Vishavkarma,
Role of actin filaments in correlating nuclear shape and cell spreading.
2014,
Pubmed
Wallingford,
Low-magnification live imaging of Xenopus embryos for cell and developmental biology.
2010,
Pubmed
,
Xenbase
Wang,
Epidermal expression of the truncated prelamin A causing Hutchinson-Gilford progeria syndrome: effects on keratinocytes, hair and skin.
2008,
Pubmed
Webster,
Sizing up the nucleus: nuclear shape, size and nuclear-envelope assembly.
2009,
Pubmed
Wiggan,
Cofilin Regulates Nuclear Architecture through a Myosin-II Dependent Mechanotransduction Module.
2017,
Pubmed
Wühr,
The Nuclear Proteome of a Vertebrate.
2015,
Pubmed
,
Xenbase
Yang,
An absence of both lamin B1 and lamin B2 in keratinocytes has no effect on cell proliferation or the development of skin and hair.
2011,
Pubmed
Zwerger,
Myopathic lamin mutations impair nuclear stability in cells and tissue and disrupt nucleo-cytoskeletal coupling.
2013,
Pubmed
