Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
1990 Oct 01;1010:5408-15. doi: 10.1128/mcb.10.10.5408-5415.1990.
Show Gene links
Show Anatomy links
GLI3 encodes a 190-kilodalton protein with multiple regions of GLI similarity.
Ruppert JM
,
Vogelstein B
,
Arheden K
,
Kinzler KW
.
???displayArticle.abstract???
The GLI oncogene, discovered by virtue of its amplification in human tumors, encodes a sequence-specific DNA-binding protein containing five zinc fingers. We have now characterized one member of a family of GLI-related zinc finger genes. A previously identified fragment of GLI3 genomic DNA was used to localize GLI3 to chromosome 7p13 and to isolate cDNA clones. Sequence analysis of these clones and identification of the GLI3 protein by using polyclonal antisera demonstrated that GLI3 encodes a protein of 1,596 amino acids and an apparent molecular mass of 190 kilodaltons. Amino acid sequence comparison with GLI demonstrated seven regions of similarity (53 to 88% identity), with the zinc fingers representing the most similar region. Furthermore, when produced in vitro, the GLI3 protein bound specifically to genomic DNA fragments containing GLI-binding sites. Amino acid sequence comparison with the product of another member of the GLI family, the Drosophila segment polarity gene cubitus interruptus Dominant, revealed additional similarity that was not shared with GLI. These studies suggest that the GLI-related genes encode a family of DNA-binding proteins with related target sequence specificities. In addition, sequence similarity aside from the zinc finger region suggests that other aspects of function are shared among the members of this gene family.
Andrews,
Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera-2. Differentiation in vivo and in vitro.
1984, Pubmed
Andrews,
Pluripotent embryonal carcinoma clones derived from the human teratocarcinoma cell line Tera-2. Differentiation in vivo and in vitro.
1984,
Pubmed
Arheden,
Chromosome localization of the human oncogene INT1 to 12q13 by in situ hybridization.
1988,
Pubmed
Arheden,
In situ hybridization localizes the human putative oncogene GLI to chromosome subbands 12q13.3-14.1.
1989,
Pubmed
Barber,
Modification of fos proteins: phosphorylation of c-fos, but not v-fos, is stimulated by 12-tetradecanoyl-phorbol-13-acetate and serum.
1987,
Pubmed
Bigner,
Chromosomal progression of malignant human gliomas from biopsy to establishment as permanent lines in vitro.
1987,
Pubmed
Brown,
The primary structure of transcription factor TFIIIA has 12 consecutive repeats.
1985,
Pubmed
,
Xenbase
Celano,
Effect of polyamine depletion on c-myc expression in human colon carcinoma cells.
1988,
Pubmed
Chavrier,
A gene encoding a protein with zinc fingers is activated during G0/G1 transition in cultured cells.
1988,
Pubmed
Chiu,
Jun-B differs in its biological properties from, and is a negative regulator of, c-Jun.
1989,
Pubmed
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Chou,
Empirical predictions of protein conformation.
1978,
Pubmed
Christy,
A gene activated in mouse 3T3 cells by serum growth factors encodes a protein with "zinc finger" sequences.
1988,
Pubmed
Collins,
Continuous growth and differentiation of human myeloid leukaemic cells in suspension culture.
1977,
Pubmed
Dasmahapatra,
pBD7, a novel cell-free expression vector with efficient translation initiation signal.
1987,
Pubmed
Evans,
Zinc fingers: gilt by association.
1988,
Pubmed
Feinberg,
"A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
1984,
Pubmed
Fürst,
Copper activates metallothionein gene transcription by altering the conformation of a specific DNA binding protein.
1988,
Pubmed
Gazdar,
Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties.
1985,
Pubmed
Giniger,
Transcription in yeast activated by a putative amphipathic alpha helix linked to a DNA binding unit.
,
Pubmed
Gubler,
A simple and very efficient method for generating cDNA libraries.
1983,
Pubmed
Kinzler,
Identification of an amplified, highly expressed gene in a human glioma.
1987,
Pubmed
Kinzler,
The GLI gene encodes a nuclear protein which binds specific sequences in the human genome.
1990,
Pubmed
Kinzler,
The GLI gene is a member of the Kruppel family of zinc finger proteins.
1988,
Pubmed
,
Xenbase
Kouzarides,
Behind the Fos and Jun leucine zipper.
1989,
Pubmed
Kozak,
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.
1987,
Pubmed
Kraft,
Using mini-prep plasmid DNA for sequencing double stranded templates with Sequenase.
1988,
Pubmed
Landschulz,
The leucine zipper: a hypothetical structure common to a new class of DNA binding proteins.
1988,
Pubmed
Laughon,
Sequence of a Drosophila segmentation gene: protein structure homology with DNA-binding proteins.
,
Pubmed
Leibovitz,
Classification of human colorectal adenocarcinoma cell lines.
1976,
Pubmed
Lipman,
Rapid and sensitive protein similarity searches.
1985,
Pubmed
Ma,
A new class of yeast transcriptional activators.
1987,
Pubmed
Milbrandt,
A nerve growth factor-induced gene encodes a possible transcriptional regulatory factor.
1987,
Pubmed
,
Xenbase
Miller,
Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Morishita,
Retroviral activation of a novel gene encoding a zinc finger protein in IL-3-dependent myeloid leukemia cell lines.
1988,
Pubmed
,
Xenbase
Murre,
A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins.
1989,
Pubmed
Orenic,
Cloning and characterization of the segment polarity gene cubitus interruptus Dominant of Drosophila.
1990,
Pubmed
,
Xenbase
Ptashne,
How eukaryotic transcriptional activators work.
1988,
Pubmed
Roberts,
Amplification of the gli gene in childhood sarcomas.
1989,
Pubmed
Robertson,
Homoeo boxes, POU proteins and the limits to promiscuity.
1988,
Pubmed
Rønne,
Double synchronization of human lymphocyte cultures: selection for high-resolution banded metaphases in the first and second division.
1985,
Pubmed
Ruppert,
The GLI-Kruppel family of human genes.
1988,
Pubmed
Sauer,
Homology among DNA-binding proteins suggests use of a conserved super-secondary structure.
1982,
Pubmed
Schütte,
jun-B inhibits and c-fos stimulates the transforming and trans-activating activities of c-jun.
1989,
Pubmed
Stanley,
Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins.
1984,
Pubmed
Sukhatme,
A zinc finger-encoding gene coregulated with c-fos during growth and differentiation, and after cellular depolarization.
1988,
Pubmed
Tabor,
DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.
1987,
Pubmed
Vinson,
Scissors-grip model for DNA recognition by a family of leucine zipper proteins.
1989,
Pubmed
Vogelstein,
Rapid purification of DNA from agarose gels by centrifugation through a disposable plastic column.
1987,
Pubmed
Wilusz,
Functional analysis of point mutations in the AAUAAA motif of the SV40 late polyadenylation signal.
1989,
Pubmed
