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Zebrafish olfactomedin 1 regulates retinal axon elongation in vivo and is a modulator of Wnt signaling pathway.
Nakaya N
,
Lee HS
,
Takada Y
,
Tzchori I
,
Tomarev SI
.
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Olfactomedin 1 (Olfm1) is a secreted glycoprotein belonging to a family of olfactomedin domain-containing proteins. It is involved in the regulation of neural crest production in chicken and promotes neuronal differentiation in Xenopus. Here, we investigate the functions of Olfm1 in zebrafish eye development. Overexpression of full-length Olfm1, and especially its BMY form lacking the olfactomedin domain, increased the thickness of the optic nerve and produced a more extended projection field in the optic tectum compared with control embryos. In contrast, injection of olfm1-morpholino oligonucleotide (Olfm1-MO) reduced the eye size, inhibited optic nerve extension, and increased the number of apoptotic cells in the retinal ganglion cell and inner nuclear layers. Overexpression of full-length Olfm1 increased the lateral separation of the expression domains of eye-field markers, rx3 and six3. The Olfm1-MO had the opposite effect. These data suggest that zebrafish Olfm1 may play roles in the early eye determination, differentiation, optic nerve extension, and branching of the retinal ganglion cellaxon terminals, with the N-terminal region of Olfm1 being critical for these effects. Injection of RNA encoding WIF-1, a secreted inhibitor of Wnt signaling, caused changes in the expression pattern of rx3 similar to those observed after Olfm1-MO injection. Simultaneous overexpression of WIF-1 and Olfm1 abolished the WIF-1 effect. Physical interaction of WIF-1 and Olfm1 was demonstrated by coimmunoprecipitation experiments. We concluded that Olfm1 serves as a modulator of Wnt signaling.
Barembaum,
Noelin-1 is a secreted glycoprotein involved in generation of the neural crest.
2000, Pubmed,
Xenbase
Barembaum,
Noelin-1 is a secreted glycoprotein involved in generation of the neural crest.
2000,
Pubmed
,
Xenbase
Burns,
Isolation and characterization of runxa and runxb, zebrafish members of the runt family of transcriptional regulators.
2002,
Pubmed
Cheng,
Pancortin-2 interacts with WAVE1 and Bcl-xL in a mitochondria-associated protein complex that mediates ischemic neuronal death.
2007,
Pubmed
Chuang,
Expression of three Rx homeobox genes in embryonic and adult zebrafish.
1999,
Pubmed
Danielson,
Four structurally distinct neuron-specific olfactomedin-related glycoproteins produced by differential promoter utilization and alternative mRNA splicing from a single gene.
1994,
Pubmed
Ekker,
Distinct expression and shared activities of members of the hedgehog gene family of Xenopus laevis.
1995,
Pubmed
,
Xenbase
Eshed,
Gliomedin mediates Schwann cell-axon interaction and the molecular assembly of the nodes of Ranvier.
2005,
Pubmed
Funayama,
SNPs and interaction analyses of noelin 2, myocilin, and optineurin genes in Japanese patients with open-angle glaucoma.
2006,
Pubmed
Hemish,
Nitric oxide activates diverse signaling pathways to regulate gene expression.
2003,
Pubmed
Hillier,
Amassin, an olfactomedin protein, mediates the massive intercellular adhesion of sea urchin coelomocytes.
2003,
Pubmed
Houart,
Establishment of the telencephalon during gastrulation by local antagonism of Wnt signaling.
2002,
Pubmed
Hsieh,
A new secreted protein that binds to Wnt proteins and inhibits their activities.
1999,
Pubmed
,
Xenbase
Hunter,
The extracellular matrix component WIF-1 is expressed during, and can modulate, retinal development.
2004,
Pubmed
Joe,
Accumulation of mutant myocilins in ER leads to ER stress and potential cytotoxicity in human trabecular meshwork cells.
2003,
Pubmed
Kataoka,
Cloning and embryonic expression patterns of the zebrafish Runt domain genes, runxa and runxb.
2000,
Pubmed
Kim,
Eye field requires the function of Sfrp1 as a Wnt antagonist.
2007,
Pubmed
Lee,
Olfactomedin-2 mediates development of the anterior central nervous system and head structures in zebrafish.
2008,
Pubmed
Li,
A single morphogenetic field gives rise to two retina primordia under the influence of the prechordal plate.
1997,
Pubmed
,
Xenbase
Liu,
The glycoprotein hGC-1 binds to cadherin and lectins.
2006,
Pubmed
Liu,
Reversal of mutant myocilin non-secretion and cell killing: implications for glaucoma.
2004,
Pubmed
Martí,
Distribution of Sonic hedgehog peptides in the developing chick and mouse embryo.
1995,
Pubmed
Moreno,
The secreted glycoprotein Noelin-1 promotes neurogenesis in Xenopus.
2001,
Pubmed
,
Xenbase
Moreno,
Noelins modulate the timing of neuronal differentiation during development.
2005,
Pubmed
,
Xenbase
Moreno,
Neural expression of mouse Noelin-1/2 and comparison with other vertebrates.
2002,
Pubmed
,
Xenbase
Nagano,
Differentially expressed olfactomedin-related glycoproteins (Pancortins) in the brain.
1998,
Pubmed
Nagano,
A2-Pancortins (Pancortin-3 and -4) are the dominant pancortins during neocortical development.
2000,
Pubmed
Nakaya,
Expression patterns of alternative transcripts of the zebrafish olfactomedin 1 genes.
2007,
Pubmed
,
Xenbase
Patthy,
The WIF module.
2000,
Pubmed
Pézeron,
Duplicate sfrp1 genes in zebrafish: sfrp1a is dynamically expressed in the developing central nervous system, gut and lateral line.
2006,
Pubmed
Rorick,
PP2A:B56epsilon is required for eye induction and eye field separation.
2007,
Pubmed
,
Xenbase
Sakuragi,
Functional analysis of chick ONT1 reveals distinguishable activities among olfactomedin-related signaling factors.
2006,
Pubmed
,
Xenbase
Shimamura,
Longitudinal organization of the anterior neural plate and neural tube.
1995,
Pubmed
Snyder,
Olfactomedin: purification, characterization, and localization of a novel olfactory glycoprotein.
1991,
Pubmed
Stigloher,
Segregation of telencephalic and eye-field identities inside the zebrafish forebrain territory is controlled by Rx3.
2006,
Pubmed
Stone,
Identification of a gene that causes primary open angle glaucoma.
1997,
Pubmed
Tendeng,
Cloning and embryonic expression of five distinct sfrp genes in the zebrafish Danio rerio.
2006,
Pubmed
Torrado,
Optimedin: a novel olfactomedin-related protein that interacts with myocilin.
2002,
Pubmed
Toyama,
Nodal induces ectopic goosecoid and lim1 expression and axis duplication in zebrafish.
1995,
Pubmed
Tsuda,
Dorsalization of the neural tube by Xenopus tiarin, a novel patterning factor secreted by the flanking nonneural head ectoderm.
2002,
Pubmed
,
Xenbase
Veroni,
beta-dystrobrevin, a kinesin-binding receptor, interacts with the extracellular matrix components pancortins.
2007,
Pubmed
Volynski,
Latrophilin fragments behave as independent proteins that associate and signal on binding of LTX(N4C).
2004,
Pubmed
Yoda,
Mutations affecting retinotectal axonal pathfinding in Medaka, Oryzias latipes.
2004,
Pubmed
Zeng,
Elucidation of subfamily segregation and intramolecular coevolution of the olfactomedin-like proteins by comprehensive phylogenetic analysis and gene expression pattern assessment.
2005,
Pubmed
Zilinski,
Expression of FoxE4 and Rx visualizes the timing and dynamics of critical processes taking place during initial stages of vertebrate eye development.
2004,
Pubmed
