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EMBO J
1987 Feb 01;62:477-83. doi: 10.1002/j.1460-2075.1987.tb04778.x.
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Enzymatic conversion of guanosine 3' adjacent to the anticodon of yeast tRNAPhe to N1-methylguanosine and the wye nucleoside: dependence on the anticodon sequence.
Droogmans L
,
Grosjean H
.
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N1-Methylguanosine (m1G) or wye nucleoside (Y) are found 3' adjacent to the anticodon (position 37) of eukaryotic tRNAPhe. The biosynthesis of these two modified nucleosides has been investigated. The importance of the type of nucleosides in the anticodon of yeast tRNAPhe on the potentiality of this tRNA to be a substrate for the corresponding maturation enzyme has also been studied. This involved microinjection into Xenopus laevis oocytes and incubation in a yeast extract of restructured yeast tRNAPhe in which the anticodon GmAA and the 3' adjacent Y nucleoside were substituted by various tetranucleotides ending with a guanosine. The results obtained by oocyte microinjection indicate: that all the restructured yeast tRNAsPhe are efficient substrates for the tRNA (guanosine-37 N1)methyltransferase. This means that the anticodon sequence is not critical for the tRNA recognition by this enzyme; in contrast, for Y nucleoside biosynthesis, the anticodon sequence GAA is an absolute requirement; the conversion of G-37 into Y-37 nucleoside is a multienzymatic process in which m1G-37 is the first obligatory intermediate; all the corresponding enzymes are cytoplasmic. In a crude yeast extract, restructured yeast tRNAPhe with G-37 is efficiently modified only into m1G-37; the corresponding enzyme is a S-adenosyl-L-methionine-dependent tRNA methyltransferase. The pure Escherichia coli tRNA (guanosine-37 N1) methyltransferase is unable to modify the guanosine-37 of yeast tRNAPhe.
Blobstein,
Structure of the fluorescent nucleoside of yeast phenylalanine transfer ribonucleic acid.
1975, Pubmed
Blobstein,
Structure of the fluorescent nucleoside of yeast phenylalanine transfer ribonucleic acid.
1975,
Pubmed
Bruce,
Enzymatic replacement of the anticodon of yeast phenylalanine transfer ribonucleic acid.
1982,
Pubmed
Bruce,
Replacement of anticodon loop nucleotides to produce functional tRNAs: amber suppressors derived from yeast tRNAPhe.
1982,
Pubmed
Carbon,
Site-directed in vitro replacement of nucleosides in the anticodon loop of tRNA: application to the study of structural requirements for queuine insertase activity.
1983,
Pubmed
,
Xenbase
Carbon,
Enzymatic replacement in vitro of the first anticodon base of yeast tRNAAsp: application to the study of tRNA maturation in vivo, after microinjection into frog oocytes.
1982,
Pubmed
De Robertis,
Intracellular transport of microinjected 5S and small nuclear RNAs.
1982,
Pubmed
,
Xenbase
Droogmans,
Enzymatic 2'-O-methylation of the wobble nucleoside of eukaryotic tRNAPhe: specificity depends on structural elements outside the anticodon loop.
1986,
Pubmed
,
Xenbase
Feinberg,
Isolation and characterization of peroxy-Y base from phenylalanine transfer ribonucleic acid of the plant, Lupinus luteus.
1974,
Pubmed
Feldmann,
Transfer ribonucleic acid from Mycoplasma laidlawii A.
1971,
Pubmed
Fournier,
Post-transcriptional modification of the wobble nucleotide in anticodon-substituted yeast tRNAArgII after microinjection into Xenopus laevis oocytes.
1983,
Pubmed
,
Xenbase
Gatica,
Aminoacylation of transfer RNA microinjected into Xenopus laevis oocytes.
1975,
Pubmed
,
Xenbase
Gillam,
The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose.
1967,
Pubmed
Haumont,
Enzymatic conversion of adenosine to inosine in the wobble position of yeast tRNAAsp: the dependence on the anticodon sequence.
1984,
Pubmed
,
Xenbase
Hjalmarsson,
Purification and characterization of transfer RNA (guanine-1)methyltransferase from Escherichia coli.
1983,
Pubmed
Kasai,
Isolation of hydroxy-Y base from rat liver tRNAPhe.
1979,
Pubmed
Kasai,
Structure of wye (Yt base) and wyosine (Yt) from Torulopsis utilis phenylalanine transfer ribonucleic acid.
1976,
Pubmed
Kuchino,
Changes of post-transcriptional modification of wye base in tumor-specific tRNAPhe.
1982,
Pubmed
Li,
Biosynthetic studies of the Y base in yeast phenylalanine tRNA. Incorporation of guanine.
1973,
Pubmed
Maelicke,
The structure of the anticodon loop of tRNAPhe from yeast as deduced from spectroscopic studies on oligonucleotides.
1975,
Pubmed
Mazabraud,
The nucleotide sequence of phenylalanine tRNA of Xenopus laevis.
1982,
Pubmed
,
Xenbase
Münch,
Biosynthesis of the nucleoside Y in yeast tRNAPhe: incorporation of the 3-amino-3-carboxypropyl-group from methionine.
1975,
Pubmed
Nakanishi,
Structure of the "peroxy-Y base" from liver tRNA Phe .
1971,
Pubmed
Pergolizzi,
Incorporation of lysine into Y base of phenylalanine tRNA in Vero cells.
1979,
Pubmed
Silberklang,
Use of in vitro 32P labeling in the sequence analysis of nonradioactive tRNAs.
1979,
Pubmed
Sindhuphak,
Site specificities of three transfer RNA methyltransferases from yeast.
1985,
Pubmed
Smith,
Nuclear magnetic resonance signal assignments of purified [13C]methyl-enriched yeast phenylalanine transfer ribonucleic acid.
1985,
Pubmed
Smolar,
Two transfer RNA (1-methylguanine) methylases from yeast.
1975,
Pubmed
Sprinzl,
Compilation of tRNA sequences.
1985,
Pubmed
Thiebe,
A specific modification next to the anticodon of phenylalanine transfer ribonucleic acid.
1968,
Pubmed
