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PLoS One
2008 Jul 16;37:e2692. doi: 10.1371/journal.pone.0002692.
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Major histocompatibility complex based resistance to a common bacterial pathogen of amphibians.
Barribeau SM
,
Villinger J
,
Waldman B
.
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Given their well-developed systems of innate and adaptive immunity, global population declines of amphibians are particularly perplexing. To investigate the role of the major histocompatibility complex (MHC) in conferring pathogen resistance, we challenged Xenopus laevis tadpoles bearing different combinations of four MHC haplotypes (f, g, j, and r) with the bacterial pathogen Aeromonas hydrophila in two experiments. In the first, we exposed ff, fg, gg, gj, and jj tadpoles, obtained from breeding MHC homozygous parents, to one of three doses of A. hydrophila or heat-killed bacteria as a control. In the second, we exposed ff, fg, fr, gg, rg, and rr tadpoles, obtained from breeding MHC heterozygous parents and subsequently genotyped by PCR, to A. hydrophila, heat-killed bacteria or media alone as controls. We thereby determined whether the same patterns of MHC resistance emerged within as among families, independent of non-MHC heritable differences. Tadpoles with r or g MHC haplotypes were more likely to die than were those with f or j haplotypes. Growth rates varied among MHC types, independent of exposure dose. Heterozygous individuals with both susceptible and resistant haplotypes were intermediate to either homozygous genotype in both size and survival. The effect of the MHC on growth and survival was consistent between experiments and across families. MHC alleles differentially confer resistance to, or tolerance of, the bacterial pathogen, which affects tadpoles' growth and survival.
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18629002
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Figure 1. Mortality as a function of bacterial dose and MHC genotype among families.(A) Percent mortality of tadpoles exposed to the control (3.0Ã106 cfu/ml heat-killed), low (1.0Ã106 cfu/ml), medium (2.5Ã106 cfu/ml), and high (3.0Ã106 cfu/ml) doses of A. hydrophila. Nâ=â90 in each treatment. (B) Percent mortality of tadpoles from each MHC genotype that were exposed to each dose of live A. hydrophila or the control. Nâ=â15 in each condition.
Figure 2. Survival with time as a function of bacterial dose, MHC genotype, and clutch order among families.Kaplan-Meier plots showing the survival of (A) tadpoles exposed to the control (3.0Ã106 cfu/ml heat-killed), low (1.0Ã106 cfu/ml), medium (2.5Ã106 cfu/ml), and high (3.0Ã106 cfu/ml) doses of A. hydrophila; (B) tadpoles exposed to the control or A. hydrophila (all doses combined); (C) tadpoles from each MHC genotype; and (D) tadpoles from early and late clutches.
Figure 3. Growth as a function of MHC genotype among families.Body length (XÌ Â±SE) at day 25 of tadpoles from each genotype exposed to the pathogen A. hydrophila and the control.
Figure 4. Mortality as a function of bacterial exposure and MHC genotype within families.(A) Percent mortality of tadpoles exposed to live (exposed) and heat-killed (control) A. hydrophila. Nâ=â120 for each treatment. (B) Percent mortality of tadpoles with each MHC genotype from 3 different families. Sample sizes differed among families; see Table 1.
Figure 5. Survival with time as a function of bacterial exposure and MHC genotype within families.Kaplan-Meier plots showing the survival of (A) tadpoles exposed to live (exposed) or heat-killed (control) A. hydrophila, and (B) tadpoles with different MHC genotypes. Vertical lines indicate exposure days.
Figure 6. Growth as a function of bacterial exposure.Body length (XÌ Â±SE) of tadpoles exposed to live A. hydrophila, heat-killed bacteria and no bacteria (controls) over time.
Figure 7. Growth as a function of MHC genotype within families.(A) Total and (B) body length (XÌ Â±SE) of tadpoles on day 18 with different MHC genotypes that were either exposed to live or heat-killed A. hydrophila as a control.
Acevedo-Whitehouse,
Genetic resistance to bovine tuberculosis in the Iberian wild boar.
2005, Pubmed
Acevedo-Whitehouse,
Genetic resistance to bovine tuberculosis in the Iberian wild boar.
2005,
Pubmed
Acevedo-Whitehouse,
Inbreeding: Disease susceptibility in California sea lions.
2003,
Pubmed
Apanius,
The nature of selection on the major histocompatibility complex.
1997,
Pubmed
Berger,
Chytridiomycosis causes amphibian mortality associated with population declines in the rain forests of Australia and Central America.
1998,
Pubmed
Bernatchez,
MHC studies in nonmodel vertebrates: what have we learned about natural selection in 15 years?
2003,
Pubmed
Briles,
Marek's disease: effects of B histocompatibility alloalleles in resistant and susceptible chicken lines.
1977,
Pubmed
Carey,
Infectious disease and worldwide declines of amphibian populations, with comments on emerging diseases in coral reef organisms and in humans.
2000,
Pubmed
Carey,
Amphibian declines: an immunological perspective.
1999,
Pubmed
,
Xenbase
Carrington,
HLA and HIV-1: heterozygote advantage and B*35-Cw*04 disadvantage.
1999,
Pubmed
Cunningham,
Pathological and microbiological findings from incidents of unusual mortality of the common frog (Rana temporaria).
1996,
Pubmed
Doherty,
Enhanced immunological surveillance in mice heterozygous at the H-2 gene complex.
1975,
Pubmed
Du Pasquier,
The immune system of Xenopus.
1989,
Pubmed
,
Xenbase
Dziminski,
Fitness consequences of variable maternal provisioning in quacking frogs (Crinia georgiana).
2006,
Pubmed
Dziminski,
Patterns and fitness consequences of intraclutch variation in egg provisioning in tropical Australian frogs.
2005,
Pubmed
Flajnik,
Changes in the immune system during metamorphosis of Xenopus.
1987,
Pubmed
,
Xenbase
Flajnik,
Two ancient allelic lineages at the single classical class I locus in the Xenopus MHC.
1999,
Pubmed
,
Xenbase
Frye,
An unusual epizootic of anuran aeromoniasis.
1985,
Pubmed
,
Xenbase
Gantress,
Development and characterization of a model system to study amphibian immune responses to iridoviruses.
2003,
Pubmed
,
Xenbase
Green,
Epizootiology of sixty-four amphibian morbidity and mortality events in the USA, 1996-2001.
2002,
Pubmed
Grimholt,
MHC polymorphism and disease resistance in Atlantic salmon (Salmo salar); facing pathogens with single expressed major histocompatibility class I and class II loci.
2003,
Pubmed
Hazen,
Prevalence and distribution of Aeromonas hydrophila in the United States.
1978,
Pubmed
Hill,
Common west African HLA antigens are associated with protection from severe malaria.
1991,
Pubmed
Hird,
Aeromonas hydrophila in wild-caught frogs and tadpoles (Rana pipiens) in Minnesota.
1981,
Pubmed
Houlahan,
Quantitative evidence for global amphibian population declines.
2000,
Pubmed
Kaplan,
THE IMPLICATIONS OF OVUM SIZE VARIABILITY FOR OFFSPRING FITNESS AND CLUTCH SIZE WITHIN SEVERAL POPULATIONS OF SALAMANDERS (AMBYSTOMA).
1980,
Pubmed
Langefors,
Association between major histocompatibility complex class IIB alleles and resistance to Aeromonas salmonicida in Atlantic salmon.
2001,
Pubmed
Lewis,
Pathogen resistance as the origin of kin altruism.
1998,
Pubmed
Lips,
Emerging infectious disease and the loss of biodiversity in a Neotropical amphibian community.
2006,
Pubmed
Liu,
Xenopus class II A genes: studies of genetics, polymorphism, and expression.
2002,
Pubmed
,
Xenbase
Lohm,
Experimental evidence for major histocompatibility complex-allele-specific resistance to a bacterial infection.
2002,
Pubmed
Mauel,
Bacterial pathogens isolated from cultured bullfrogs (Rana castesbeiana).
2002,
Pubmed
McClelland,
Major histocompatibility complex heterozygote superiority during coinfection.
2003,
Pubmed
Mendelson,
Biodiversity. Confronting amphibian declines and extinctions.
2006,
Pubmed
Nauciel,
Role of H-2 and non-H-2 genes in control of bacterial clearance from the spleen in Salmonella typhimurium-infected mice.
1988,
Pubmed
Nonaka,
Major histocompatibility complex gene mapping in the amphibian Xenopus implies a primordial organization.
1997,
Pubmed
,
Xenbase
Pearman,
Response of the Italian agile frog (Rana latastei) to a Ranavirus, frog virus 3: a model for viral emergence in naïve populations.
2004,
Pubmed
Penn,
MHC heterozygosity confers a selective advantage against multiple-strain infections.
2002,
Pubmed
Piertney,
The evolutionary ecology of the major histocompatibility complex.
2006,
Pubmed
Pitcher,
MHC class IIB alleles contribute to both additive and nonadditive genetic effects on survival in Chinook salmon.
2006,
Pubmed
Råberg,
Disentangling genetic variation for resistance and tolerance to infectious diseases in animals.
2007,
Pubmed
Rachowicz,
Emerging infectious disease as a proximate cause of amphibian mass mortality.
2006,
Pubmed
Retallick,
Endemic infection of the amphibian chytrid fungus in a frog community post-decline.
2004,
Pubmed
Rollins-Smith,
Antimicrobial peptide defenses against pathogens associated with global amphibian declines.
2002,
Pubmed
Rollins-Smith,
Activity of antimicrobial skin peptides from ranid frogs against Batrachochytrium dendrobatidis, the chytrid fungus associated with global amphibian declines.
2002,
Pubmed
Rollins-Smith,
Antimicrobial Peptide defenses in amphibian skin.
2005,
Pubmed
Rollins-Smith,
Metamorphosis and the amphibian immune system.
1998,
Pubmed
Rollins-Smith,
Neuroendocrine-immune system interactions in amphibians: implications for understanding global amphibian declines.
2001,
Pubmed
Rollins-Smith,
Antimicrobial peptide defenses of the mountain yellow-legged frog (Rana muscosa).
2006,
Pubmed
Salter-Cid,
Expression of MHC class Ia and class Ib during ontogeny: high expression in epithelia and coregulation of class Ia and lmp7 genes.
1998,
Pubmed
,
Xenbase
Stuart,
Status and trends of amphibian declines and extinctions worldwide.
2004,
Pubmed
Tamouza,
Infectious complications in sickle cell disease are influenced by HLA class II alleles.
2002,
Pubmed
Taylor,
Effects of malathion on disease susceptibility in Woodhouse's toads.
1999,
Pubmed
Todd,
Parasites lost? An overlooked hypothesis for the evolution of alternative reproductive strategies in amphibians.
2007,
Pubmed
Villinger,
Self-referent MHC type matching in frog tadpoles.
2008,
Pubmed
,
Xenbase
Westerdahl,
Associations between malaria and MHC genes in a migratory songbird.
2005,
Pubmed
Wilbur,
Ecological Aspects of Amphibian Metamorphosis: Nonnormal distributions of competitive ability reflect selection for facultative metamorphosis.
1973,
Pubmed
