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EMBO J
2001 Nov 15;2022:6383-93. doi: 10.1093/emboj/20.22.6383.
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The N-terminus of histone H2B, but not that of histone H3 or its phosphorylation, is essential for chromosome condensation.
de la Barre AE
,
Angelov D
,
Molla A
,
Dimitrov S
.
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We have studied the role of individual histone N-termini and the phosphorylation of histone H3 in chromosome condensation. Nucleosomes, reconstituted with histone octamers containing different combinations of recombinant full-length and tailless histones, were used as competitors for chromosome assembly in Xenopus egg extracts. Nucleosomes reconstituted with intact octamers inhibited chromosome condensation as efficiently as the native ones, while tailless nucleosomes were unable to affect this process. Importantly, the addition to the extract of particles containing only intact histone H2B strongly interfered with chromosome formation while such an effect was not observed with particles lacking the N-terminal tail of H2B. This demonstrates that the inhibition effect observed in the presence of competitor nucleosomes is mainly due to the N-terminus of this histone, which, therefore, is essential for chromosome condensation. Nucleosomes in which all histones but H3 were tailless did not impede chromosome formation. In addition, when competitor nucleosome particles were reconstituted with full-length H2A, H2B and H4 and histone H3 mutated at the phosphorylable serine 10 or serine 28, their inhibiting efficiency was identical to that of the native particles. Hence, the tail of H3, whether intact or phosphorylated, is not important for chromosome condensation. A novel hypothesis, termed 'the ready production label' was suggested to explain the role of histone H3 phosphorylation during cell division.
Adams,
Essential roles of Drosophila inner centromere protein (INCENP) and aurora B in histone H3 phosphorylation, metaphase chromosome alignment, kinetochore disjunction, and chromosome segregation.
2001, Pubmed
Adams,
Essential roles of Drosophila inner centromere protein (INCENP) and aurora B in histone H3 phosphorylation, metaphase chromosome alignment, kinetochore disjunction, and chromosome segregation.
2001,
Pubmed
Arents,
The nucleosomal core histone octamer at 3.1 A resolution: a tripartite protein assembly and a left-handed superhelix.
1991,
Pubmed
Banères,
The N tails of histones H3 and H4 adopt a highly structured conformation in the nucleosome.
1997,
Pubmed
Barra,
Histone H1 is dispensable for methylation-associated gene silencing in Ascobolus immersus and essential for long life span.
2000,
Pubmed
Bradbury,
Reversible histone modifications and the chromosome cell cycle.
1992,
Pubmed
Bradbury,
Phosphorylation of very-lysine-rich histone in Physarum polycephalum. Correlation with chromosome condensation.
1973,
Pubmed
Cheung,
Signaling to chromatin through histone modifications.
2000,
Pubmed
Cobb,
Meiotic events at the centromeric heterochromatin: histone H3 phosphorylation, topoisomerase II alpha localization and chromosome condensation.
1999,
Pubmed
Dasso,
Nuclear assembly is independent of linker histones.
1994,
Pubmed
,
Xenbase
de la Barre,
Assembly of mitotic chromosomes in Xenopus egg extract.
1999,
Pubmed
,
Xenbase
de la Barre,
Core histone N-termini play an essential role in mitotic chromosome condensation.
2000,
Pubmed
,
Xenbase
Dimitrov,
Remodeling somatic nuclei in Xenopus laevis egg extracts: molecular mechanisms for the selective release of histones H1 and H1(0) from chromatin and the acquisition of transcriptional competence.
1996,
Pubmed
,
Xenbase
Dimitrov,
Remodeling sperm chromatin in Xenopus laevis egg extracts: the role of core histone phosphorylation and linker histone B4 in chromatin assembly.
1994,
Pubmed
,
Xenbase
Dimitrov,
Chromatin and nuclear assembly: experimental approaches towards the reconstitution of transcriptionally active and silent states.
1995,
Pubmed
Encontre,
Chromatin core particle obtained by selective cleavage of histones H3 and H4 by clostripain.
1988,
Pubmed
Giet,
Drosophila aurora B kinase is required for histone H3 phosphorylation and condensin recruitment during chromosome condensation and to organize the central spindle during cytokinesis.
2001,
Pubmed
Hans,
Histone H3 phosphorylation and cell division.
2001,
Pubmed
Hendzel,
Mitosis-specific phosphorylation of histone H3 initiates primarily within pericentromeric heterochromatin during G2 and spreads in an ordered fashion coincident with mitotic chromosome condensation.
1997,
Pubmed
Hirano,
Condensins, chromosome condensation protein complexes containing XCAP-C, XCAP-E and a Xenopus homolog of the Drosophila Barren protein.
1997,
Pubmed
,
Xenbase
Hirano,
Topoisomerase II does not play a scaffolding role in the organization of mitotic chromosomes assembled in Xenopus egg extracts.
1993,
Pubmed
,
Xenbase
Hirano,
Chromosome cohesion, condensation, and separation.
2000,
Pubmed
Hirano,
A heterodimeric coiled-coil protein required for mitotic chromosome condensation in vitro.
1994,
Pubmed
,
Xenbase
Houchmandzadeh,
Elasticity measurements show the existence of thin rigid cores inside mitotic chromosomes.
1999,
Pubmed
,
Xenbase
Houchmandzadeh,
Elasticity and structure of eukaryote chromosomes studied by micromanipulation and micropipette aspiration.
1997,
Pubmed
Hsu,
Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes.
2000,
Pubmed
Kaszás,
Phosphorylation of histone H3 is correlated with changes in the maintenance of sister chromatid cohesion during meiosis in maize, rather than the condensation of the chromatin.
2000,
Pubmed
Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase
Losada,
Identification and characterization of SA/Scc3p subunits in the Xenopus and human cohesin complexes.
2000,
Pubmed
,
Xenbase
Luger,
Expression and purification of recombinant histones and nucleosome reconstitution.
1999,
Pubmed
Luger,
Crystal structure of the nucleosome core particle at 2.8 A resolution.
1997,
Pubmed
Mahadevan,
Rapid histone H3 phosphorylation in response to growth factors, phorbol esters, okadaic acid, and protein synthesis inhibitors.
1991,
Pubmed
Mirzabekov,
Chromatin superstructure-dependent crosslinking with DNA of the histone H5 residues Thr1, His25 and His62.
1990,
Pubmed
Murnion,
Chromatin-associated protein phosphatase 1 regulates aurora-B and histone H3 phosphorylation.
2001,
Pubmed
,
Xenbase
Mutskov,
Persistent interactions of core histone tails with nucleosomal DNA following acetylation and transcription factor binding.
1998,
Pubmed
Ohsumi,
Chromosome condensation in Xenopus mitotic extracts without histone H1.
1993,
Pubmed
,
Xenbase
Philpott,
Sperm decondensation in Xenopus egg cytoplasm is mediated by nucleoplasmin.
1991,
Pubmed
,
Xenbase
Poirier,
Reversible and irreversible unfolding of mitotic newt chromosomes by applied force.
2000,
Pubmed
Saitoh,
ScII: an abundant chromosome scaffold protein is a member of a family of putative ATPases with an unusual predicted tertiary structure.
1994,
Pubmed
Sauvé,
Phosphorylation-induced rearrangement of the histone H3 NH2-terminal domain during mitotic chromosome condensation.
1999,
Pubmed
Scrittori,
pEg2 aurora-A kinase, histone H3 phosphorylation, and chromosome assembly in Xenopus egg extract.
2001,
Pubmed
,
Xenbase
Shen,
Linker histone H1 regulates specific gene expression but not global transcription in vivo.
1996,
Pubmed
Shen,
Linker histones are not essential and affect chromatin condensation in vivo.
1995,
Pubmed
Speliotes,
The survivin-like C. elegans BIR-1 protein acts with the Aurora-like kinase AIR-2 to affect chromosomes and the spindle midzone.
2000,
Pubmed
Strahl,
The language of covalent histone modifications.
2000,
Pubmed
Sutani,
Fission yeast condensin complex: essential roles of non-SMC subunits for condensation and Cdc2 phosphorylation of Cut3/SMC4.
1999,
Pubmed
,
Xenbase
Thomson,
MAP kinase-mediated signalling to nucleosomes and immediate-early gene induction.
1999,
Pubmed
Thomson,
The nucleosomal response associated with immediate-early gene induction is mediated via alternative MAP kinase cascades: MSK1 as a potential histone H3/HMG-14 kinase.
1999,
Pubmed
Turner,
Histone acetylation and an epigenetic code.
2000,
Pubmed
Van Hooser,
Histone H3 phosphorylation is required for the initiation, but not maintenance, of mammalian chromosome condensation.
1998,
Pubmed
Wei,
Phosphorylation of histone H3 at serine 10 is correlated with chromosome condensation during mitosis and meiosis in Tetrahymena.
1998,
Pubmed
Wei,
Phosphorylation of histone H3 is required for proper chromosome condensation and segregation.
1999,
Pubmed
Wolffe,
Chromatin disruption and modification.
1999,
Pubmed
