Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
PKA modulation of Kv4.2-encoded A-type potassium channels requires formation of a supramolecular complex.
Schrader LA
,
Anderson AE
,
Mayne A
,
Pfaffinger PJ
,
Sweatt JD
.
???displayArticle.abstract???
A-type channels, encoded by the pore-forming alpha-subunits of the Kv4.x family, are particularly important in regulating membrane excitability in the CNS and the heart. Given the key role of modulation of A currents by kinases, we sought to investigate the protein structure-function relationships underlying the regulation of these currents by PKA. We have previously shown the existence of two PKA phosphorylation sites in the Kv4.2 sequence; therefore, we focused this study on the Kv4.2 primary subunit. In the present studies we made the surprising finding that PKA phosphorylation of the Kv4.2 alpha-subunit is necessary but not sufficient for channel modulation; channel modulation by PKA required the presence of an ancillary subunit, the K+ channel interacting protein (KChIP3). Therefore, these findings indicate a surprising complexity to kinase regulation of A currents, in that an interaction of two separate molecular events, alpha-subunit phosphorylation and the association of an ancillary subunit (KChIP3), are necessary for phosphorylation-dependent regulation of Kv4.2-encoded A channels by PKA. Overall, our studies indicate that PKA must of necessity act on a supramolecular complex of pore-forming alpha-subunits plus ancillary subunits to alter channel properties.
Adams,
The A-type potassium channel Kv4.2 is a substrate for the mitogen-activated protein kinase ERK.
2000, Pubmed
Adams,
The A-type potassium channel Kv4.2 is a substrate for the mitogen-activated protein kinase ERK.
2000,
Pubmed
An,
Modulation of A-type potassium channels by a family of calcium sensors.
2000,
Pubmed
,
Xenbase
Anderson,
Kv4.2 phosphorylation by cyclic AMP-dependent protein kinase.
2000,
Pubmed
Baldwin,
Characterization of a mammalian cDNA for an inactivating voltage-sensitive K+ channel.
1991,
Pubmed
,
Xenbase
Beck,
Remodelling inactivation gating of Kv4 channels by KChIP1, a small-molecular-weight calcium-binding protein.
2002,
Pubmed
,
Xenbase
Blair,
Functional characterization of RK5, a voltage-gated K+ channel cloned from the rat cardiovascular system.
1991,
Pubmed
,
Xenbase
Bourque,
Transient calcium-dependent potassium current in magnocellular neurosecretory cells of the rat supraoptic nucleus.
1988,
Pubmed
Buxbaum,
Calsenilin: a calcium-binding protein that interacts with the presenilins and regulates the levels of a presenilin fragment.
1998,
Pubmed
Carrión,
DREAM is a Ca2+-regulated transcriptional repressor.
1999,
Pubmed
Chabala,
Low molecular weight poly(A)+ mRNA species encode factors that modulate gating of a non-Shaker A-type K+ channel.
1993,
Pubmed
,
Xenbase
Covarrubias,
Elimination of rapid potassium channel inactivation by phosphorylation of the inactivation gate.
1994,
Pubmed
,
Xenbase
Decher,
hKChIP2 is a functional modifier of hKv4.3 potassium channels: cloning and expression of a short hKChIP2 splice variant.
2001,
Pubmed
,
Xenbase
Fisher,
Properties of the transient K+ current in acutely isolated supraoptic neurons from adult rat.
1998,
Pubmed
Frangioni,
Use of a general purpose mammalian expression vector for studying intracellular protein targeting: identification of critical residues in the nuclear lamin A/C nuclear localization signal.
1993,
Pubmed
Hakes,
New vectors for high level expression of recombinant proteins in bacteria.
1992,
Pubmed
Heinemann,
Molecular and functional characterization of a rat brain Kv beta 3 potassium channel subunit.
1995,
Pubmed
,
Xenbase
Hoffman,
Downregulation of transient K+ channels in dendrites of hippocampal CA1 pyramidal neurons by activation of PKA and PKC.
1998,
Pubmed
Hoffman,
Neuromodulation of dendritic action potentials.
1999,
Pubmed
Hoffman,
K+ channel regulation of signal propagation in dendrites of hippocampal pyramidal neurons.
1997,
Pubmed
Holmqvist,
Kinetic modulation of Kv4-mediated A-current by arachidonic acid is dependent on potassium channel interacting proteins.
2001,
Pubmed
,
Xenbase
Jan,
Structural elements involved in specific K+ channel functions.
1992,
Pubmed
Jerng,
K+ channel inactivation mediated by the concerted action of the cytoplasmic N- and C-terminal domains.
1997,
Pubmed
,
Xenbase
Jerng,
Inactivation gating of Kv4 potassium channels: molecular interactions involving the inner vestibule of the pore.
1999,
Pubmed
,
Xenbase
Jin,
Phosphorylation-dependent functional coupling of hSlo calcium-dependent potassium channel and its hbeta 4 subunit.
2002,
Pubmed
Jonas,
Regulation of potassium channels by protein kinases.
1996,
Pubmed
Leicher,
Coexpression of the KCNA3B gene product with Kv1.5 leads to a novel A-type potassium channel.
1998,
Pubmed
Levin,
Phosphorylation of a K+ channel alpha subunit modulates the inactivation conferred by a beta subunit. Involvement of cytoskeleton.
1996,
Pubmed
,
Xenbase
Levitan,
Modulation of ion channels by protein phosphorylation and dephosphorylation.
1994,
Pubmed
Liss,
Tuning pacemaker frequency of individual dopaminergic neurons by Kv4.3L and KChip3.1 transcription.
2001,
Pubmed
Ma,
ER transport signals and trafficking of potassium channels and receptors.
2002,
Pubmed
Maletic-Savatic,
Differential spatiotemporal expression of K+ channel polypeptides in rat hippocampal neurons developing in situ and in vitro.
1995,
Pubmed
Morohashi,
Molecular cloning and characterization of CALP/KChIP4, a novel EF-hand protein interacting with presenilin 2 and voltage-gated potassium channel subunit Kv4.
2002,
Pubmed
Murakoshi,
Phosphorylation of the Kv2.1 K+ channel alters voltage-dependent activation.
1997,
Pubmed
Nagaya,
Potassium channel alpha and beta subunits assemble in the endoplasmic reticulum.
1997,
Pubmed
Pongs,
Molecular biology of voltage-dependent potassium channels.
1992,
Pubmed
Quirk,
Experience-dependent changes in extracellular spike amplitude may reflect regulation of dendritic action potential back-propagation in rat hippocampal pyramidal cells.
2001,
Pubmed
Rettig,
Inactivation properties of voltage-gated K+ channels altered by presence of beta-subunit.
1994,
Pubmed
,
Xenbase
Roberson,
The mitogen-activated protein kinase cascade couples PKA and PKC to cAMP response element binding protein phosphorylation in area CA1 of hippocampus.
1999,
Pubmed
Rudy,
At least two mRNA species contribute to the properties of rat brain A-type potassium channels expressed in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Schorderet-Slatkine,
Forskolin increases cAMP and inhibits progesterone induced meiosis reinitiation in Xenopus laevis oocytes.
1982,
Pubmed
,
Xenbase
Scott,
Primary structure of a beta subunit of alpha-dendrotoxin-sensitive K+ channels from bovine brain.
1994,
Pubmed
Serôdio,
Identification of molecular components of A-type channels activating at subthreshold potentials.
1994,
Pubmed
,
Xenbase
Serôdio,
Differential expression of Kv4 K+ channel subunits mediating subthreshold transient K+ (A-type) currents in rat brain.
1998,
Pubmed
Serôdio,
Cloning of a novel component of A-type K+ channels operating at subthreshold potentials with unique expression in heart and brain.
1996,
Pubmed
,
Xenbase
Sheng,
Subcellular segregation of two A-type K+ channel proteins in rat central neurons.
1992,
Pubmed
,
Xenbase
Shi,
Beta subunits promote K+ channel surface expression through effects early in biosynthesis.
1996,
Pubmed
Shibata,
Expression of Kv3.1 and Kv4.2 genes in developing cerebellar granule cells.
1999,
Pubmed
Song,
Somatodendritic depolarization-activated potassium currents in rat neostriatal cholinergic interneurons are predominantly of the A type and attributable to coexpression of Kv4.2 and Kv4.1 subunits.
1998,
Pubmed
Tkatch,
Kv4.2 mRNA abundance and A-type K(+) current amplitude are linearly related in basal ganglia and basal forebrain neurons.
2000,
Pubmed
Trimmer,
Regulation of ion channel expression by cytoplasmic subunits.
1998,
Pubmed
Tsaur,
Cloning, expression and CNS distribution of Kv4.3, an A-type K+ channel alpha subunit.
1997,
Pubmed
,
Xenbase
Varga,
Input-specific immunolocalization of differentially phosphorylated Kv4.2 in the mouse brain.
2000,
Pubmed
Watanabe,
Dendritic K+ channels contribute to spike-timing dependent long-term potentiation in hippocampal pyramidal neurons.
2002,
Pubmed
Yang,
Kvbeta subunits increase expression of Kv4.3 channels by interacting with their C termini.
2001,
Pubmed
Yang,
GDNF acutely modulates excitability and A-type K(+) channels in midbrain dopaminergic neurons.
2001,
Pubmed
Yuan,
Protein kinase modulation of dendritic K+ channels in hippocampus involves a mitogen-activated protein kinase pathway.
2002,
Pubmed
