Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Yeast proteins that recognize nuclear localization sequences.
Silver P
,
Sadler I
,
Osborne MA
.
???displayArticle.abstract???
A variety of peptides can mediate the localization of proteins to the nucleus. We have identified yeast proteins of 70 and 59 kD that bind to nuclear localization peptides of SV-40 T antigen, Xenopus nucleoplasmin, and the yeast proteins Ga14 and histone H2B. These proteins are assayed by the binding of peptide-albumin conjugates to proteins immobilized on nitrocellulose filters. These binding proteins fractionate with nuclei and are extractable with salt but not detergent. Radiolabeled peptide-albumin conjugates also bind to isolated nuclei; the binding is saturable and can be extracted with salt. Different nuclear localization peptides compete with each other, implying that a single class of proteins is responsible for their recognition. The 70- and 59-kD proteins have the properties expected for a receptor that would act to direct proteins to the nucleus.
Adam,
Identification of specific binding proteins for a nuclear location sequence.
1989, Pubmed
Adam,
Identification of specific binding proteins for a nuclear location sequence.
1989,
Pubmed
Burnette,
"Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
1981,
Pubmed
Colledge,
Extensive mutagenesis of the nuclear location signal of simian virus 40 large-T antigen.
1986,
Pubmed
Daniel,
Visualization of lipoprotein receptors by ligand blotting.
1983,
Pubmed
Dingwall,
A polypeptide domain that specifies migration of nucleoplasmin into the nucleus.
1982,
Pubmed
,
Xenbase
Dingwall,
The nucleoplasmin nuclear location sequence is larger and more complex than that of SV-40 large T antigen.
1988,
Pubmed
Feldherr,
Movement of a karyophilic protein through the nuclear pores of oocytes.
1984,
Pubmed
,
Xenbase
Fletcher,
Purification and characterization of OTF-1, a transcription factor regulating cell cycle expression of a human histone H2b gene.
1987,
Pubmed
Gilliam,
Localization of the Huntington's disease gene to a small segment of chromosome 4 flanked by D4S10 and the telomere.
1987,
Pubmed
Goldfarb,
Synthetic peptides as nuclear localization signals.
,
Pubmed
,
Xenbase
Hall,
Targeting of E. coli beta-galactosidase to the nucleus in yeast.
1984,
Pubmed
Kalderon,
Sequence requirements for nuclear location of simian virus 40 large-T antigen.
,
Pubmed
Kalderon,
A short amino acid sequence able to specify nuclear location.
1984,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lanford,
Construction and characterization of an SV40 mutant defective in nuclear transport of T antigen.
1984,
Pubmed
Lanford,
Induction of nuclear transport with a synthetic peptide homologous to the SV40 T antigen transport signal.
1986,
Pubmed
Lapetina,
Specific binding of [alpha-32P]GTP to cytosolic and membrane-bound proteins of human platelets correlates with the activation of phospholipase C.
1987,
Pubmed
Lin,
GAL4 derivatives function alone and synergistically with mammalian activators in vitro.
1988,
Pubmed
Lin,
The general affinity of lac repressor for E. coli DNA: implications for gene regulation in procaryotes and eucaryotes.
1975,
Pubmed
Ma,
A new class of yeast transcriptional activators.
1987,
Pubmed
Markland,
Signal-dependent translocation of simian virus 40 large-T antigen into rat liver nuclei in a cell-free system.
1987,
Pubmed
Moreland,
Amino acid sequences that determine the nuclear localization of yeast histone 2B.
1987,
Pubmed
Nelson,
Context affects nuclear protein localization in Saccharomyces cerevisiae.
1989,
Pubmed
Newmeyer,
In vitro transport of a fluorescent nuclear protein and exclusion of non-nuclear proteins.
1986,
Pubmed
,
Xenbase
Newmeyer,
Assembly in vitro of nuclei active in nuclear protein transport: ATP is required for nucleoplasmin accumulation.
1986,
Pubmed
,
Xenbase
Newmeyer,
Nuclear import can be separated into distinct steps in vitro: nuclear pore binding and translocation.
1988,
Pubmed
,
Xenbase
Prywes,
Purification of the c-fos enhancer-binding protein.
1987,
Pubmed
Richardson,
Nuclear protein migration involves two steps: rapid binding at the nuclear envelope followed by slower translocation through nuclear pores.
1988,
Pubmed
,
Xenbase
Ruohola,
Reconstitution of protein transport from the endoplasmic reticulum to the Golgi complex in yeast: the acceptor Golgi compartment is defective in the sec23 mutant.
1988,
Pubmed
Silver,
Amino terminus of the yeast GAL4 gene product is sufficient for nuclear localization.
1984,
Pubmed
Yoneda,
Antibodies to Asp-Asp-Glu-Asp can inhibit transport of nuclear proteins into the nucleus.
1988,
Pubmed
