Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
EMBO J
2005 Oct 19;2420:3519-31. doi: 10.1038/sj.emboj.7600825.
Show Gene links
Show Anatomy links
Nup155 regulates nuclear envelope and nuclear pore complex formation in nematodes and vertebrates.
Franz C
,
Askjaer P
,
Antonin W
,
Iglesias CL
,
Haselmann U
,
Schelder M
,
de Marco A
,
Wilm M
,
Antony C
,
Mattaj IW
.
???displayArticle.abstract???
Nuclear envelope (NE) formation during cell division in multicellular organisms is a central yet poorly understood biological process. We report that the conserved nucleoporin Nup155 has an essential function in NE formation in Caenorhabditis elegans embryos and in Xenopus laevis egg extracts. In vivo depletion of Nup155 led to failure of nuclear lamina formation and defects in chromosome segregation at anaphase. Nup155 depletion inhibited accumulation of nucleoporins at the nuclear periphery, including those recruited to chromatin early in NE formation. Electron microscopy analysis revealed that Nup155 is also required for the formation of a continuous nuclear membrane in vivo and in vitro. Time-course experiments indicated that Nup155 is recruited to chromatin at the time of NE sealing, suggesting that nuclear pore complex assembly has to progress to a relatively late stage before NE membrane assembly occurs.
Aitchison,
Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p.
1995, Pubmed
Aitchison,
Two novel related yeast nucleoporins Nup170p and Nup157p: complementation with the vertebrate homologue Nup155p and functional interactions with the yeast nuclear pore-membrane protein Pom152p.
1995,
Pubmed
Antonin,
The integral membrane nucleoporin pom121 functionally links nuclear pore complex assembly and nuclear envelope formation.
2005,
Pubmed
,
Xenbase
Askjaer,
Ran GTPase cycle and importins alpha and beta are essential for spindle formation and nuclear envelope assembly in living Caenorhabditis elegans embryos.
2002,
Pubmed
Belgareh,
An evolutionarily conserved NPC subcomplex, which redistributes in part to kinetochores in mammalian cells.
2001,
Pubmed
Bodoor,
Sequential recruitment of NPC proteins to the nuclear periphery at the end of mitosis.
1999,
Pubmed
Cronshaw,
Proteomic analysis of the mammalian nuclear pore complex.
2002,
Pubmed
Finlay,
A complex of nuclear pore proteins required for pore function.
1991,
Pubmed
Finlay,
Reconstitution of biochemically altered nuclear pores: transport can be eliminated and restored.
1990,
Pubmed
,
Xenbase
Galy,
Caenorhabditis elegans nucleoporins Nup93 and Nup205 determine the limit of nuclear pore complex size exclusion in vivo.
2003,
Pubmed
,
Xenbase
Gant,
Nuclear assembly.
1997,
Pubmed
Geles,
A role for Caenorhabditis elegans importin IMA-2 in germ line and embryonic mitosis.
2002,
Pubmed
Gigliotti,
Nup154, a new Drosophila gene essential for male and female gametogenesis is related to the nup155 vertebrate nucleoporin gene.
1998,
Pubmed
Goldberg,
Dimples, pores, star-rings, and thin rings on growing nuclear envelopes: evidence for structural intermediates in nuclear pore complex assembly.
1997,
Pubmed
,
Xenbase
Grandi,
Nup93, a vertebrate homologue of yeast Nic96p, forms a complex with a novel 205-kDa protein and is required for correct nuclear pore assembly.
1997,
Pubmed
,
Xenbase
Gruenbaum,
The nuclear lamina comes of age.
2005,
Pubmed
Gurdon,
Injected nuclei in frog oocytes: fate, enlargement, and chromatin dispersal.
1976,
Pubmed
,
Xenbase
Haraguchi,
Live fluorescence imaging reveals early recruitment of emerin, LBR, RanBP2, and Nup153 to reforming functional nuclear envelopes.
2000,
Pubmed
Harel,
Removal of a single pore subcomplex results in vertebrate nuclei devoid of nuclear pores.
2003,
Pubmed
,
Xenbase
Hartl,
Nuclear assembly with lambda DNA in fractionated Xenopus egg extracts: an unexpected role for glycogen in formation of a higher order chromatin intermediate.
1994,
Pubmed
,
Xenbase
Hawryluk-Gara,
Vertebrate Nup53 interacts with the nuclear lamina and is required for the assembly of a Nup93-containing complex.
2005,
Pubmed
,
Xenbase
Kenna,
Yeast N1e3p/Nup170p is required for normal stoichiometry of FG nucleoporins within the nuclear pore complex.
1996,
Pubmed
Kerscher,
Novel role for a Saccharomyces cerevisiae nucleoporin, Nup170p, in chromosome segregation.
2001,
Pubmed
Kiger,
Developmental genetics of the essential Drosophila nucleoporin nup154: allelic differences due to an outward-directed promoter in the P-element 3' end.
1999,
Pubmed
Liu,
Essential roles for Caenorhabditis elegans lamin gene in nuclear organization, cell cycle progression, and spatial organization of nuclear pore complexes.
2000,
Pubmed
Lohka,
Formation in vitro of sperm pronuclei and mitotic chromosomes induced by amphibian ooplasmic components.
1983,
Pubmed
,
Xenbase
Lutzmann,
Reconstitution of Nup157 and Nup145N into the Nup84 complex.
2005,
Pubmed
Macaulay,
Assembly of the nuclear pore: biochemically distinct steps revealed with NEM, GTP gamma S, and BAPTA.
1996,
Pubmed
,
Xenbase
Maduro,
Identification and cloning of unc-119, a gene expressed in the Caenorhabditis elegans nervous system.
1995,
Pubmed
Mattaj,
Sorting out the nuclear envelope from the endoplasmic reticulum.
2004,
Pubmed
Maul,
Time sequence of nuclear pore formation in phytohemagglutinin-stimulated lymphocytes and in HeLa cells during the cell cycle.
1972,
Pubmed
McDonald,
Cryomethods for thin section electron microscopy.
2002,
Pubmed
Moore,
HCP-1, a protein involved in chromosome segregation, is localized to the centromere of mitotic chromosomes in Caenorhabditis elegans.
1999,
Pubmed
Müller-Reichert,
Cryoimmobilization and three-dimensional visualization of C. elegans ultrastructure.
2003,
Pubmed
Pellettieri,
Coordinate activation of maternal protein degradation during the egg-to-embryo transition in C. elegans.
2003,
Pubmed
Poteryaev,
Involvement of the actin cytoskeleton and homotypic membrane fusion in ER dynamics in Caenorhabditis elegans.
2005,
Pubmed
Powers,
Reconstituted nuclei depleted of a vertebrate GLFG nuclear pore protein, p97, import but are defective in nuclear growth and replication.
1995,
Pubmed
,
Xenbase
Radu,
Nup155 is a novel nuclear pore complex protein that contains neither repetitive sequence motifs nor reacts with WGA.
1993,
Pubmed
Rayala,
The mRNA export factor human Gle1 interacts with the nuclear pore complex protein Nup155.
2004,
Pubmed
Rout,
The yeast nuclear pore complex: composition, architecture, and transport mechanism.
2000,
Pubmed
Sheehan,
Steps in the assembly of replication-competent nuclei in a cell-free system from Xenopus eggs.
1988,
Pubmed
,
Xenbase
Shulga,
Binding dynamics of structural nucleoporins govern nuclear pore complex permeability and may mediate channel gating.
2003,
Pubmed
Strome,
Spindle dynamics and the role of gamma-tubulin in early Caenorhabditis elegans embryos.
2001,
Pubmed
Suntharalingam,
Peering through the pore: nuclear pore complex structure, assembly, and function.
2003,
Pubmed
Vasu,
Nuclear pores and nuclear assembly.
2001,
Pubmed
Walther,
The conserved Nup107-160 complex is critical for nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase
Walther,
The cytoplasmic filaments of the nuclear pore complex are dispensable for selective nuclear protein import.
2002,
Pubmed
,
Xenbase
Walther,
The nucleoporin Nup153 is required for nuclear pore basket formation, nuclear pore complex anchoring and import of a subset of nuclear proteins.
2001,
Pubmed
,
Xenbase
Wilson,
A trypsin-sensitive receptor on membrane vesicles is required for nuclear envelope formation in vitro.
1988,
Pubmed
,
Xenbase
