Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Katanin is responsible for the M-phase microtubule-severing activity in Xenopus eggs.
McNally FJ
,
Thomas S
.
???displayArticle.abstract???
Microtubules are dynamic structures whose proper rearrangement during the cell cycle is essential for the positioning of membranes during interphase and for chromosome segregation during mitosis. The previous discovery of a cyclin B/cdc2-activated microtubule-severing activity in M-phase Xenopus egg extracts suggested that a microtubule-severing protein might play an important role in cell cycle-dependent changes in microtubule dynamics and organization. However, the isolation of three different microtubule-severing proteins, p56, EF1alpha, and katanin, has only confused the issue because none of these proteins is directly activated by cyclin B/cdc2. Here we use immunodepletion with antibodies specific for a vertebrate katanin homologue to demonstrate that katanin is responsible for the majority of M-phase severing activity in Xenopus eggs. This result suggests that katanin is responsible for changes in microtubules occurring at mitosis. Immunofluorescence analysis demonstrated that katanin is concentrated at a microtubule-dependent structure at mitotic spindle poles in Xenopus A6 cells and in human fibroblasts, suggesting a specific role in microtubule disassembly at spindle poles. Surprisingly, katanin was also found in adult mouse brain, indicating that katanin may have other functions distinct from its mitotic role.
Andersen,
Mitotic chromatin regulates phosphorylation of Stathmin/Op18.
1997, Pubmed,
Xenbase
Andersen,
Mitotic chromatin regulates phosphorylation of Stathmin/Op18.
1997,
Pubmed
,
Xenbase
Baas,
A composite model for establishing the microtubule arrays of the neuron.
1996,
Pubmed
Belmont,
Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules.
1996,
Pubmed
,
Xenbase
Belmont,
Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts.
1990,
Pubmed
,
Xenbase
Clark-Maguire,
mei-1, a gene required for meiotic spindle formation in Caenorhabditis elegans, is a member of a family of ATPases.
1994,
Pubmed
Clark-Maguire,
Localization of the mei-1 gene product of Caenorhaditis elegans, a meiotic-specific spindle component.
1994,
Pubmed
Confalonieri,
A 200-amino acid ATPase module in search of a basic function.
1995,
Pubmed
Doxsey,
Pericentrin, a highly conserved centrosome protein involved in microtubule organization.
1994,
Pubmed
,
Xenbase
Félix,
Centrosome assembly in vitro: role of gamma-tubulin recruitment in Xenopus sperm aster formation.
1994,
Pubmed
,
Xenbase
Frohman,
Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer.
1988,
Pubmed
Hartman,
Katanin, a microtubule-severing protein, is a novel AAA ATPase that targets to the centrosome using a WD40-containing subunit.
1998,
Pubmed
Horio,
Visualization of the dynamic instability of individual microtubules by dark-field microscopy.
,
Pubmed
Keating,
Microtubule release from the centrosome.
1997,
Pubmed
Kennelly,
Consensus sequences as substrate specificity determinants for protein kinases and protein phosphatases.
1991,
Pubmed
Kishino,
Force measurements by micromanipulation of a single actin filament by glass needles.
1988,
Pubmed
Lennon,
The I.M.A.G.E. Consortium: an integrated molecular analysis of genomes and their expression.
1996,
Pubmed
Lin,
Malignant transformation of human fibroblast strain MSU-1.1 by v-fes requires an additional genetic change.
1995,
Pubmed
Lohret,
A role for katanin-mediated axonemal severing during Chlamydomonas deflagellation.
1998,
Pubmed
McNally,
Identification of katanin, an ATPase that severs and disassembles stable microtubules.
1993,
Pubmed
,
Xenbase
McNally,
Katanin, the microtubule-severing ATPase, is concentrated at centrosomes.
1996,
Pubmed
Merdes,
A complex of NuMA and cytoplasmic dynein is essential for mitotic spindle assembly.
1996,
Pubmed
,
Xenbase
Mitchison,
Dynamic instability of microtubule growth.
,
Pubmed
Mitchison,
Polewards microtubule flux in the mitotic spindle: evidence from photoactivation of fluorescence.
1989,
Pubmed
Moritz,
Microtubule nucleation by gamma-tubulin-containing rings in the centrosome.
1995,
Pubmed
Murray,
Cell cycle extracts.
1991,
Pubmed
Saxton,
Tubulin dynamics in cultured mammalian cells.
1984,
Pubmed
Shiina,
Microtubule severing by elongation factor 1 alpha.
1994,
Pubmed
,
Xenbase
Shiina,
A novel homo-oligomeric protein responsible for an MPF-dependent microtubule-severing activity.
1992,
Pubmed
,
Xenbase
Stearns,
In vitro reconstitution of centrosome assembly and function: the central role of gamma-tubulin.
1994,
Pubmed
,
Xenbase
Stearns,
Gamma-tubulin is a highly conserved component of the centrosome.
1991,
Pubmed
,
Xenbase
Tournebize,
Distinct roles of PP1 and PP2A-like phosphatases in control of microtubule dynamics during mitosis.
1997,
Pubmed
,
Xenbase
Tousson,
Centrophilin: a novel mitotic spindle protein involved in microtubule nucleation.
1991,
Pubmed
Vale,
Severing of stable microtubules by a mitotically activated protein in Xenopus egg extracts.
1991,
Pubmed
,
Xenbase
Walczak,
XKCM1: a Xenopus kinesin-related protein that regulates microtubule dynamics during mitotic spindle assembly.
1996,
Pubmed
,
Xenbase
Walker,
Dynamic instability of individual microtubules analyzed by video light microscopy: rate constants and transition frequencies.
1988,
Pubmed
Waters,
The kinetochore microtubule minus-end disassembly associated with poleward flux produces a force that can do work.
1996,
Pubmed
Zhai,
Microtubule dynamics at the G2/M transition: abrupt breakdown of cytoplasmic microtubules at nuclear envelope breakdown and implications for spindle morphogenesis.
1996,
Pubmed
Zhai,
Quantitative determination of the proportion of microtubule polymer present during the mitosis-interphase transition.
1994,
Pubmed
Zheng,
Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome.
1991,
Pubmed
Zheng,
Nucleation of microtubule assembly by a gamma-tubulin-containing ring complex.
1995,
Pubmed
,
Xenbase
