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Zhu W
,
Ukomadu C
,
Jha S
,
Senga T
,
Dhar SK
,
Wohlschlegel JA
,
Nutt LK
,
Kornbluth S
,
Dutta A
.
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The MCM2-7 helicase complex is loaded on DNA replication origins during the G1 phase of the cell cycle to license the origins for replication in S phase. How the initiator primase-polymerase complex, DNA polymerase alpha (pol alpha), is brought to the origins is still unclear. We show that And-1/Ctf4 (Chromosome transmission fidelity 4) interacts with Mcm10, which associates with MCM2-7, and with the p180 subunit of DNA pol alpha. And-1 is essential for DNA synthesis and the stability of p180 in mammalian cells. In Xenopus egg extracts And-1 is loaded on the chromatin after Mcm10, concurrently with DNA pol alpha, and is required for efficient DNA synthesis. Mcm10 is required for chromatin loading of And-1 and an antibody that disrupts the Mcm10-And-1 interaction interferes with the loading of And-1 and of pol alpha, inhibiting DNA synthesis. And-1/Ctf4 is therefore a new replication initiation factor that brings together the MCM2-7 helicase and the DNA pol alpha-primase complex, analogous to the linker between helicase and primase or helicase and polymerase that is seen in the bacterial replication machinery. The discovery also adds to the connection between replication initiation and sister chromatid cohesion.
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17761813
???displayArticle.pmcLink???PMC1973143 ???displayArticle.link???Genes Dev ???displayArticle.grants???[+]
Aparicio,
Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase.
1997, Pubmed
Aparicio,
Components and dynamics of DNA replication complexes in S. cerevisiae: redistribution of MCM proteins and Cdc45p during S phase.
1997,
Pubmed
Arias,
PCNA functions as a molecular platform to trigger Cdt1 destruction and prevent re-replication.
2006,
Pubmed
,
Xenbase
Bell,
Molecular biology: prime-time progress.
2006,
Pubmed
Bell,
DNA replication in eukaryotic cells.
2002,
Pubmed
Blow,
The chromosome cycle: coordinating replication and segregation. Second in the cycles review series.
2005,
Pubmed
Bowman,
Structural analysis of a eukaryotic sliding DNA clamp-clamp loader complex.
2004,
Pubmed
Chen,
Separate domains of p21 involved in the inhibition of Cdk kinase and PCNA.
1995,
Pubmed
,
Xenbase
Dornreiter,
Interaction of DNA polymerase alpha-primase with cellular replication protein A and SV40 T antigen.
1992,
Pubmed
Formosa,
Dna2 mutants reveal interactions with Dna polymerase alpha and Ctf4, a Pol alpha accessory factor, and show that full Dna2 helicase activity is not essential for growth.
1999,
Pubmed
Gambus,
GINS maintains association of Cdc45 with MCM in replisome progression complexes at eukaryotic DNA replication forks.
2006,
Pubmed
Gillespie,
Scc2 couples replication licensing to sister chromatid cohesion in Xenopus egg extracts.
2004,
Pubmed
,
Xenbase
Hanna,
Saccharomyces cerevisiae CTF18 and CTF4 are required for sister chromatid cohesion.
2001,
Pubmed
Harris,
sepB: an Aspergillus nidulans gene involved in chromosome segregation and the initiation of cytokinesis.
1995,
Pubmed
Huang,
Stoichiometry and mechanism of assembly of SV40 T antigen complexes with the viral origin of DNA replication and DNA polymerase alpha-primase.
1998,
Pubmed
Izumi,
Localization of human Mcm10 is spatially and temporally regulated during the S phase.
2004,
Pubmed
Izumi,
Cell cycle-dependent proteolysis and phosphorylation of human Mcm10.
2001,
Pubmed
Jeruzalmi,
Crystal structure of the processivity clamp loader gamma (gamma) complex of E. coli DNA polymerase III.
2001,
Pubmed
Kawasaki,
Interactions between Mcm10p and other replication factors are required for proper initiation and elongation of chromosomal DNA replication in Saccharomyces cerevisiae.
2000,
Pubmed
Kim,
tau couples the leading- and lagging-strand polymerases at the Escherichia coli DNA replication fork.
1996,
Pubmed
Köhler,
AND-1, a natural chimeric DNA-binding protein, combines an HMG-box with regulatory WD-repeats.
1997,
Pubmed
,
Xenbase
Kong,
Three-dimensional structure of the beta subunit of E. coli DNA polymerase III holoenzyme: a sliding DNA clamp.
1992,
Pubmed
Kouprina,
CTF4 (CHL15) mutants exhibit defective DNA metabolism in the yeast Saccharomyces cerevisiae.
1992,
Pubmed
Krishna,
Crystal structure of the eukaryotic DNA polymerase processivity factor PCNA.
1994,
Pubmed
Lee,
Depletion of human micro-RNA miR-125b reveals that it is critical for the proliferation of differentiated cells but not for the down-regulation of putative targets during differentiation.
2005,
Pubmed
Lei,
Two mcm3 mutations affect different steps in the initiation of DNA replication.
2002,
Pubmed
Lengronne,
Establishment of sister chromatid cohesion at the S. cerevisiae replication fork.
2006,
Pubmed
Mayer,
Identification of protein complexes required for efficient sister chromatid cohesion.
2004,
Pubmed
McHenry,
Chromosomal replicases as asymmetric dimers: studies of subunit arrangement and functional consequences.
2003,
Pubmed
Miles,
Protein affinity chromatography with purified yeast DNA polymerase alpha detects proteins that bind to DNA polymerase.
1992,
Pubmed
Miles,
Evidence that POB1, a Saccharomyces cerevisiae protein that binds to DNA polymerase alpha, acts in DNA metabolism in vivo.
1992,
Pubmed
Mimura,
Xenopus Cdc45-dependent loading of DNA polymerase alpha onto chromatin under the control of S-phase Cdk.
1998,
Pubmed
,
Xenbase
Pacek,
A requirement for MCM7 and Cdc45 in chromosome unwinding during eukaryotic DNA replication.
2004,
Pubmed
,
Xenbase
Pacek,
Localization of MCM2-7, Cdc45, and GINS to the site of DNA unwinding during eukaryotic DNA replication.
2006,
Pubmed
,
Xenbase
Petronczki,
Sister-chromatid cohesion mediated by the alternative RF-CCtf18/Dcc1/Ctf8, the helicase Chl1 and the polymerase-alpha-associated protein Ctf4 is essential for chromatid disjunction during meiosis II.
2004,
Pubmed
Ricke,
Mcm10 regulates the stability and chromatin association of DNA polymerase-alpha.
2004,
Pubmed
Saxena,
A dimerized coiled-coil domain and an adjoining part of geminin interact with two sites on Cdt1 for replication inhibition.
2004,
Pubmed
,
Xenbase
Senga,
PCNA is a cofactor for Cdt1 degradation by CUL4/DDB1-mediated N-terminal ubiquitination.
2006,
Pubmed
Soultanas,
The bacterial helicase-primase interaction: a common structural/functional module.
2005,
Pubmed
Takahashi,
Recruitment of Xenopus Scc2 and cohesin to chromatin requires the pre-replication complex.
2004,
Pubmed
,
Xenbase
Takayama,
GINS, a novel multiprotein complex required for chromosomal DNA replication in budding yeast.
2003,
Pubmed
Tsutsui,
Genetic and physical interactions between Schizosaccharomyces pombe Mcl1 and Rad2, Dna2 and DNA polymerase alpha: evidence for a multifunctional role of Mcl1 in DNA replication and repair.
2005,
Pubmed
Williams,
mcl1+, the Schizosaccharomyces pombe homologue of CTF4, is important for chromosome replication, cohesion, and segregation.
2002,
Pubmed
Wittmeyer,
The Saccharomyces cerevisiae DNA polymerase alpha catalytic subunit interacts with Cdc68/Spt16 and with Pob3, a protein similar to an HMG1-like protein.
1997,
Pubmed
Zhou,
A coordinated temporal interplay of nucleosome reorganization factor, sister chromatin cohesion factor, and DNA polymerase alpha facilitates DNA replication.
2004,
Pubmed
Zhu,
Rereplication by depletion of geminin is seen regardless of p53 status and activates a G2/M checkpoint.
2004,
Pubmed
