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Am J Physiol Renal Physiol
2011 May 01;3005:F1089-95. doi: 10.1152/ajprenal.00610.2010.
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Second transmembrane domain modulates epithelial sodium channel gating in response to shear stress.
Abi-Antoun T
,
Shi S
,
Tolino LA
,
Kleyman TR
,
Carattino MD
.
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Na(+) absorption and K(+) secretion in the distal segments of the nephron are modulated by the tubular flow rate. Epithelial Na(+) channels (ENaC), composed of α-, β-, and γ-subunits respond to laminar shear stress (LSS) with an increase in open probability. Higher vertebrates express a δ-ENaC subunit that is functionally related to the α-subunit, while sharing only 35% of sequence identity. We investigated the response of δβγ channels to LSS. Both the time course and magnitude of activation of δβγ channels by LSS were remarkably different from those of αβγ channels. ENaC subunits have similar topology, with an extracellular region connected by two transmembrane domains with intracellular N and C termini. To identify the specific domains that are responsible for the differences in the response to flow of αβγ and δβγ channels, we generated a series of α-δ chimeras and site-specific α-subunit mutants and examined parameters of activation by LSS. We found that specific sites in the region encompassing and just preceding the second transmembrane domain were responsible for the differences in the magnitude and time course of channel activation by LSS.
Althaus,
Mechano-sensitivity of epithelial sodium channels (ENaCs): laminar shear stress increases ion channel open probability.
2007, Pubmed,
Xenbase
Althaus,
Mechano-sensitivity of epithelial sodium channels (ENaCs): laminar shear stress increases ion channel open probability.
2007,
Pubmed
,
Xenbase
Bianchi,
The neurotoxic MEC-4(d) DEG/ENaC sodium channel conducts calcium: implications for necrosis initiation.
2004,
Pubmed
,
Xenbase
Bruns,
Epithelial Na+ channels are fully activated by furin- and prostasin-dependent release of an inhibitory peptide from the gamma-subunit.
2007,
Pubmed
,
Xenbase
Canessa,
Amiloride-sensitive epithelial Na+ channel is made of three homologous subunits.
1994,
Pubmed
,
Xenbase
Canessa,
Membrane topology of the epithelial sodium channel in intact cells.
1994,
Pubmed
,
Xenbase
Carattino,
Mutations in the pore region modify epithelial sodium channel gating by shear stress.
2005,
Pubmed
,
Xenbase
Carattino,
Epithelial Na+ channels are activated by laminar shear stress.
2004,
Pubmed
,
Xenbase
Carattino,
The epithelial Na+ channel is inhibited by a peptide derived from proteolytic processing of its alpha subunit.
2006,
Pubmed
,
Xenbase
Chalfie,
Neurosensory mechanotransduction.
2009,
Pubmed
Collier,
Extracellular chloride regulates the epithelial sodium channel.
2009,
Pubmed
,
Xenbase
Collier,
Extracellular protons regulate human ENaC by modulating Na+ self-inhibition.
2009,
Pubmed
,
Xenbase
Giraldez,
Cloning and functional expression of a new epithelial sodium channel delta subunit isoform differentially expressed in neurons of the human and monkey telencephalon.
2007,
Pubmed
,
Xenbase
Good,
Luminal influences on potassium secretion: sodium concentration and fluid flow rate.
1979,
Pubmed
Huang,
Gene interactions affecting mechanosensory transduction in Caenorhabditis elegans.
1994,
Pubmed
Jasti,
Structure of acid-sensing ion channel 1 at 1.9 A resolution and low pH.
2007,
Pubmed
Kashlan,
Constraint-based, homology model of the extracellular domain of the epithelial Na+ channel α subunit reveals a mechanism of channel activation by proteases.
2011,
Pubmed
Kashlan,
On the interaction between amiloride and its putative alpha-subunit epithelial Na+ channel binding site.
2005,
Pubmed
,
Xenbase
Kellenberger,
Epithelial sodium channel/degenerin family of ion channels: a variety of functions for a shared structure.
2002,
Pubmed
Khuri,
Effects of flow rate and potassium intake on distal tubular potassium transfer.
1975,
Pubmed
Kleyman,
ENaC at the cutting edge: regulation of epithelial sodium channels by proteases.
2009,
Pubmed
Liechti,
A combined computational and functional approach identifies new residues involved in pH-dependent gating of ASIC1a.
2010,
Pubmed
Liu,
Ca2+ dependence of flow-stimulated K secretion in the mammalian cortical collecting duct.
2007,
Pubmed
Malnic,
Flow dependence of K+ secretion in cortical distal tubules of the rat.
1989,
Pubmed
Mano,
DEG/ENaC channels: a touchy superfamily that watches its salt.
1999,
Pubmed
Morimoto,
Mechanism underlying flow stimulation of sodium absorption in the mammalian collecting duct.
2006,
Pubmed
,
Xenbase
Passero,
Conformational changes associated with proton-dependent gating of ASIC1a.
2009,
Pubmed
,
Xenbase
Paukert,
Candidate amino acids involved in H+ gating of acid-sensing ion channel 1a.
2008,
Pubmed
,
Xenbase
Satlin,
Regulation of cation transport in the distal nephron by mechanical forces.
2006,
Pubmed
Satlin,
Epithelial Na(+) channels are regulated by flow.
2001,
Pubmed
,
Xenbase
Schild,
Identification of amino acid residues in the alpha, beta, and gamma subunits of the epithelial sodium channel (ENaC) involved in amiloride block and ion permeation.
1997,
Pubmed
,
Xenbase
Sheng,
Furin cleavage activates the epithelial Na+ channel by relieving Na+ self-inhibition.
2006,
Pubmed
,
Xenbase
Sheng,
Extracellular histidine residues crucial for Na+ self-inhibition of epithelial Na+ channels.
2004,
Pubmed
,
Xenbase
Sheng,
Functional role of extracellular loop cysteine residues of the epithelial Na+ channel in Na+ self-inhibition.
2007,
Pubmed
,
Xenbase
Snyder,
Membrane topology of the amiloride-sensitive epithelial sodium channel.
1994,
Pubmed
,
Xenbase
Waldmann,
Molecular cloning and functional expression of a novel amiloride-sensitive Na+ channel.
1995,
Pubmed
,
Xenbase
Woda,
Flow-dependent K+ secretion in the cortical collecting duct is mediated by a maxi-K channel.
2001,
Pubmed
