Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Mol Cell Biol
2002 Aug 01;2216:5688-97. doi: 10.1128/MCB.22.16.5688-5697.2002.
Show Gene links
Show Anatomy links
Involvement of histone methylation and phosphorylation in regulation of transcription by thyroid hormone receptor.
Li J
,
Lin Q
,
Yoon HG
,
Huang ZQ
,
Strahl BD
,
Allis CD
,
Wong J
.
???displayArticle.abstract???
Previous studies have established an important role of histone acetylation in transcriptional control by nuclear hormone receptors. With chromatin immunoprecipitation assays, we have now investigated whether histone methylation and phosphorylation are also involved in transcriptional regulation by thyroid hormone receptor (TR). We found that repression by unliganded TR is associated with a substantial increase in methylation of H3 lysine 9 (H3-K9) and a decrease in methylation of H3 lysine 4 (H3-K4), methylation of H3 arginine 17 (H3-R17), and a dual modification of phosphorylation of H3 serine 10 and acetylation of lysine 14 (pS10/acK14). On the other hand, transcriptional activation by liganded TR is coupled with a substantial decrease in both H3-K4 and H3-K9 methylation and a robust increase in H3-R17 methylation and the dual modification of pS10/acK14. Trichostatin A treatment results in not only histone hyperacetylation but also an increase in methylation of H3-K4, increase in dual modification of pS10/acK14, and reduction in methylation of H3-K9, revealing an extensive interplay between histone acetylation, methylation, and phosphorylation. In an effort to understand the underlying mechanism for an increase in H3-K9 methylation during repression by unliganded TR, we demonstrated that TR interacts in vitro with an H3-K9-specific histone methyltransferase (HMT), SUV39H1. Functional analysis indicates that SUV39H1 can facilitate repression by unliganded TR and in so doing requires its HMT activity. Together, our data uncover a novel role of H3-K9 methylation in repression by unliganded TR and provide strong evidence for the involvement of multiple distinct histone covalent modifications (acetylation, methylation, and phosphorylation) in transcriptional control by nuclear hormone receptors.
Almouzni,
Replication-coupled chromatin assembly is required for the repression of basal transcription in vivo.
1993, Pubmed,
Xenbase
Almouzni,
Replication-coupled chromatin assembly is required for the repression of basal transcription in vivo.
1993,
Pubmed
,
Xenbase
Bannister,
Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain.
2001,
Pubmed
Bauer,
Methylation at arginine 17 of histone H3 is linked to gene activation.
2002,
Pubmed
Blanco,
The histone acetylase PCAF is a nuclear receptor coactivator.
1998,
Pubmed
Boggs,
Differentially methylated forms of histone H3 show unique association patterns with inactive human X chromosomes.
2002,
Pubmed
Briggs,
Histone H3 lysine 4 methylation is mediated by Set1 and required for cell growth and rDNA silencing in Saccharomyces cerevisiae.
2001,
Pubmed
Chen,
Synergistic, p160 coactivator-dependent enhancement of estrogen receptor function by CARM1 and p300.
2000,
Pubmed
Chen,
Regulation of hormone-induced histone hyperacetylation and gene activation via acetylation of an acetylase.
1999,
Pubmed
Chen,
A transcriptional co-repressor that interacts with nuclear hormone receptors.
1995,
Pubmed
Chen,
Regulation of transcription by a protein methyltransferase.
1999,
Pubmed
Cheung,
Synergistic coupling of histone H3 phosphorylation and acetylation in response to epidermal growth factor stimulation.
2000,
Pubmed
Glass,
The coregulator exchange in transcriptional functions of nuclear receptors.
2000,
Pubmed
Grunstein,
Histone acetylation in chromatin structure and transcription.
1997,
Pubmed
Guenther,
A core SMRT corepressor complex containing HDAC3 and TBL1, a WD40-repeat protein linked to deafness.
2000,
Pubmed
Heinzel,
A complex containing N-CoR, mSin3 and histone deacetylase mediates transcriptional repression.
1997,
Pubmed
Hörlein,
Ligand-independent repression by the thyroid hormone receptor mediated by a nuclear receptor co-repressor.
1995,
Pubmed
Hsu,
Mitotic phosphorylation of histone H3 is governed by Ipl1/aurora kinase and Glc7/PP1 phosphatase in budding yeast and nematodes.
2000,
Pubmed
Hu,
Transcriptional repression by nuclear hormone receptors.
2000,
Pubmed
Jenuwein,
Translating the histone code.
2001,
Pubmed
Jepsen,
Combinatorial roles of the nuclear receptor corepressor in transcription and development.
2000,
Pubmed
Koh,
Synergistic enhancement of nuclear receptor function by p160 coactivators and two coactivators with protein methyltransferase activities.
2001,
Pubmed
Lachner,
Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins.
2001,
Pubmed
Li,
Both corepressor proteins SMRT and N-CoR exist in large protein complexes containing HDAC3.
2000,
Pubmed
,
Xenbase
Li,
Specific targeting and constitutive association of histone deacetylase complexes during transcriptional repression.
2002,
Pubmed
,
Xenbase
Li,
p300 stimulates transcription instigated by ligand-bound thyroid hormone receptor at a step subsequent to chromatin disruption.
1999,
Pubmed
,
Xenbase
Li,
p300 requires its histone acetyltransferase activity and SRC-1 interaction domain to facilitate thyroid hormone receptor activation in chromatin.
2000,
Pubmed
,
Xenbase
Litt,
Correlation between histone lysine methylation and developmental changes at the chicken beta-globin locus.
2001,
Pubmed
Lo,
Phosphorylation of serine 10 in histone H3 is functionally linked in vitro and in vivo to Gcn5-mediated acetylation at lysine 14.
2000,
Pubmed
Lo,
Snf1--a histone kinase that works in concert with the histone acetyltransferase Gcn5 to regulate transcription.
2001,
Pubmed
Ma,
Hormone-dependent, CARM1-directed, arginine-specific methylation of histone H3 on a steroid-regulated promoter.
2001,
Pubmed
Mangelsdorf,
The nuclear receptor superfamily: the second decade.
1995,
Pubmed
McKenna,
Nuclear receptor coregulators: cellular and molecular biology.
1999,
Pubmed
MURRAY,
THE OCCURRENCE OF EPSILON-N-METHYL LYSINE IN HISTONES.
1964,
Pubmed
Nagy,
Nuclear receptor repression mediated by a complex containing SMRT, mSin3A, and histone deacetylase.
1997,
Pubmed
Nielsen,
Rb targets histone H3 methylation and HP1 to promoters.
2001,
Pubmed
Nishioka,
Set9, a novel histone H3 methyltransferase that facilitates transcription by precluding histone tail modifications required for heterochromatin formation.
2002,
Pubmed
Noma K,
Transitions in distinct histone H3 methylation patterns at the heterochromatin domain boundaries.
2001,
Pubmed
Ordentlich,
Corepressors and nuclear hormone receptor function.
2001,
Pubmed
Rea,
Regulation of chromatin structure by site-specific histone H3 methyltransferases.
2000,
Pubmed
Roth,
Histone acetyltransferases.
2001,
Pubmed
Schurter,
Methylation of histone H3 by coactivator-associated arginine methyltransferase 1.
2001,
Pubmed
Shang,
Cofactor dynamics and sufficiency in estrogen receptor-regulated transcription.
2000,
Pubmed
Strahl,
The language of covalent histone modifications.
2000,
Pubmed
Strahl,
Methylation of histone H3 at lysine 4 is highly conserved and correlates with transcriptionally active nuclei in Tetrahymena.
1999,
Pubmed
Strahl,
Set2 is a nucleosomal histone H3-selective methyltransferase that mediates transcriptional repression.
2002,
Pubmed
Struhl,
Histone acetylation and transcriptional regulatory mechanisms.
1998,
Pubmed
Tachibana,
Set domain-containing protein, G9a, is a novel lysine-preferring mammalian histone methyltransferase with hyperactivity and specific selectivity to lysines 9 and 27 of histone H3.
2001,
Pubmed
Urnov,
A necessary good: nuclear hormone receptors and their chromatin templates.
2001,
Pubmed
Vandel,
Transcriptional repression by the retinoblastoma protein through the recruitment of a histone methyltransferase.
2001,
Pubmed
Wade,
A multiple subunit Mi-2 histone deacetylase from Xenopus laevis cofractionates with an associated Snf2 superfamily ATPase.
1998,
Pubmed
,
Xenbase
Wang,
Purification and functional characterization of a histone H3-lysine 4-specific methyltransferase.
2001,
Pubmed
Wang,
Methylation of histone H4 at arginine 3 facilitating transcriptional activation by nuclear hormone receptor.
2001,
Pubmed
,
Xenbase
Wolffe,
Activators and repressors: making use of chromatin to regulate transcription.
1997,
Pubmed
Wolffe,
Chromatin disruption and modification.
1999,
Pubmed
Wong,
Distinct requirements for chromatin assembly in transcriptional repression by thyroid hormone receptor and histone deacetylase.
1998,
Pubmed
,
Xenbase
Wong,
A role for nucleosome assembly in both silencing and activation of the Xenopus TR beta A gene by the thyroid hormone receptor.
1995,
Pubmed
,
Xenbase
Wong,
Transcriptional repression by the SMRT-mSin3 corepressor: multiple interactions, multiple mechanisms, and a potential role for TFIIB.
1998,
Pubmed
Workman,
Alteration of nucleosome structure as a mechanism of transcriptional regulation.
1998,
Pubmed
Xue,
NURD, a novel complex with both ATP-dependent chromatin-remodeling and histone deacetylase activities.
1998,
Pubmed
Zhang,
Histone deacetylases and SAP18, a novel polypeptide, are components of a human Sin3 complex.
1997,
Pubmed
Zhang,
The dermatomyositis-specific autoantigen Mi2 is a component of a complex containing histone deacetylase and nucleosome remodeling activities.
1998,
Pubmed
