Dev Dyn 2007 Jun 01;2366:1633-49. doi: 10.1002/dvdy.21167.

Xenopus cDNA microarray identification of genes with endodermal organ expression.

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The endoderm is classically defined as the innermost layer of three Metazoan germ layers. During organogenesis, the endoderm gives rise to the digestive and respiratory tracts as well as associated organs such as the liver, pancreas, and lung. At present, however, how the endoderm forms the variety of cell types of digestive and respiratory tracts as well as the budding organs is not well understood. In order to investigate the molecular basis and mechanism of organogenesis and to identify the endodermal organ-related marker genes, we carried out microarray analysis using Xenopus cDNA chips. To achieve this goal, we isolated the Xenopus gut endoderm from three different stages of Xenopus organogenesis, and separated each stage of gut endoderm into anterior and posterior regions. Competitive hybridization of cDNA between the anterior and posterior endoderm regions, to screen genes that specifically expressed in the major organs, revealed 915 candidates. We then selected 104 clones for in situ hybridization analysis. Here, we report the identification and expression patterns of the 104 Xenopus endodermal genes, which would serve as useful markers for studying endodermal organ development.

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Species referenced: Xenopus laevis
Genes referenced: a2ml1 ag1 anxa1 apoe atp1b3 bmpr1a btf3 c8b ca2 cd82 cers2 cers3 cfap52 cldn4 cnfn.1 col4a5 crebrf ddx3x desi2 dstn entpd2 epcam etv1 fetub fst fzd7 gata6 gpd2 hdac1 hes4 hmgn1 hmgn2 hrg hspd1 iars1 id4 ids ift88 impdh2 jund kng1 mafb meis1 msn myc ndufaf3 nom1 nr2f2 odc1 oxct1 pfkm rab3d rfx3 rpl6 sec61b sema6d sephs1 serbp1 serpinb10 sfrp1 slc6a14.2 sox2 stard10 tbx2 tspan1 tubb usf1 xbp1 zbtb46

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