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Total RNA and poly(A)+ RNA were isolated from tissues and cultured cells of various mammalian species (bovine muzzle epidermis and bladder urothelium; rat hepatoma cells; human cell lines HeLa, MCF-7 and A-431) and examined by translation in vitro using the reticulocyte lysate system. Polypeptides were separated and identified by two-dimensional electrophoresis and cytokeratins were selectively enriched from the translation assays by co-polymerization with added heterologous cytokeratins. In all three species, non-epidermal cytokeratins A, D and mol. wt. 40,000 (corresponding to numbers 8, 18 and 19 of the human cytokeratin catalog of Moll et al., 1982) were identified as translation products capable of co-polymerization with epidermal keratins. Several other basic and other acidic cytokeratins were also identified as translational products. In addition, two unidentified polypeptides (mol. wt. 52,000 and 43,000) which were minor polypeptides in cytoskeletons and translation assays were found to be specifically enriched in co-polymers with bovine epidermal keratins. The results indicate that many, perhaps all, non-epidermal cytokeratins characteristic of simple epithelia are genuine products of translation and that their diversity is not due to post-translational modification or processing. These findings, taken together with observations of in vitro translation of epidermal mRNAs, suggest that the diversity of cell type-specific expression of the different members of the cytokeratin polypeptide family is largely due to the cell type-specific synthesis of diverse mRNAs.
Bennett,
Differences among 100-A filamentilament subunits from different cell types.
1978, Pubmed
Bennett,
Differences among 100-A filamentilament subunits from different cell types.
1978,
Pubmed
Bladon,
Prekeratin biosynthesis in human scalp epidermis.
1982,
Pubmed
Bravo,
Architecture and polypeptide composition of HeLa cytoskeletons. Modification of cytoarchitectural polypeptides during mitosis.
1982,
Pubmed
Czosnek,
Studies on the biosynthesis of neurofilament proteins.
1980,
Pubmed
Davison,
Classes of distinguishable 10 nm cytoplasmic filaments.
1977,
Pubmed
Dodemont,
The genes coding for the cytoskeletal proteins actin and vimentin in warm-blooded vertebrates.
1982,
Pubmed
Fey,
Differential immunological crossreactivity of HeLa keratin antibodies with human epidermal keratins.
1983,
Pubmed
Franke,
Intermediate-sized filaments of the prekeratin type in myoepithelial cells.
1980,
Pubmed
Franke,
Differentiation-related patterns of expression of proteins of intermediate-size filaments in tissues and cultured cells.
1982,
Pubmed
Franke,
Diversity of cytokeratins. Differentiation specific expression of cytokeratin polypeptides in epithelial cells and tissues.
1981,
Pubmed
Franke,
Differences of expression of cytoskeletal proteins in cultured rat hepatocytes and hepatoma cells.
1981,
Pubmed
Franke,
Biochemical and immunological identification of cytokeratin proteins present in hepatocytes of mammalian liver tissue.
1981,
Pubmed
Franke,
Permanently proliferating rat vascular smooth muscle cell with maintained expression of smooth muscle characteristics, including actin of the vascular smooth muscle type.
1980,
Pubmed
Franke,
Antibody to prekeratin. Decoration of tonofilament like arrays in various cells of epithelial character.
1978,
Pubmed
Franke,
HeLa cells contain intermediate-sized filaments of the prekeratin type.
1979,
Pubmed
Franke,
Different intermediate-sized filaments distinguished by immunofluorescence microscopy.
1978,
Pubmed
Fuchs,
Multiple keratins of cultured human epidermal cells are translated from different mRNA molecules.
1979,
Pubmed
Fuchs,
Changes in keratin gene expression during terminal differentiation of the keratinocyte.
1980,
Pubmed
Geisler,
The amino acid sequence of chicken muscle desmin provides a common structural model for intermediate filament proteins.
1982,
Pubmed
Geisler,
Comparison of the proteins of two immunologically distinct intermediate-sized filaments by amino acid sequence analysis: desmin and vimentin.
1981,
Pubmed
Gibbs,
Epidermal keratin messenger RNAs: a heterogeneous family.
1982,
Pubmed
Gilmartin,
Phosphorylation of epidermal keratins.
1980,
Pubmed
Gown,
Monoclonal antibodies to intermediate filament proteins of human cells: unique and cross-reacting antibodies.
1982,
Pubmed
Hubbard,
Isolation of rat hepatocyte plasma membranes. II. Identification of membrane-associated cytoskeletal proteins.
1983,
Pubmed
Kreis,
De novo synthesis and specific assembly of keratin filaments in nonepithelial cells after microinjection of mRNA for epidermal keratin.
1983,
Pubmed
Lazarides,
Intermediate filaments: a chemically heterogeneous, developmentally regulated class of proteins.
1982,
Pubmed
Leim,
Chemistry and biology of neuronal and glial intermediate filaments.
1982,
Pubmed
Moll,
The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells.
1982,
Pubmed
O'Connor,
In vitro translation of the intermediate filament proteins desmin and vimentin.
1981,
Pubmed
O'Farrell,
High resolution two-dimensional electrophoresis of proteins.
1975,
Pubmed
O'Farrell,
High resolution two-dimensional electrophoresis of basic as well as acidic proteins.
1977,
Pubmed
Osborn,
Intermediate filaments.
1982,
Pubmed
Pruss,
All classes of intermediate filaments share a common antigenic determinant defined by a monoclonal antibody.
1981,
Pubmed
Quinlan,
Heteropolymer filaments of vimentin and desmin in vascular smooth muscle tissue and cultured baby hamster kidney cells demonstrated by chemical crosslinking.
1982,
Pubmed
Quinlan,
Molecular interactions in intermediate-sized filaments revealed by chemical cross-linking. Heteropolymers of vimentin and glial filament protein in cultured human glioma cells.
1983,
Pubmed
Roop,
Keratin gene expression in mouse epidermis and cultured epidermal cells.
1983,
Pubmed
Schiller,
A subfamily of relatively large and basic cytokeratin polypeptides as defined by peptide mapping is represented by one or several polypeptides in epithelial cells.
1982,
Pubmed
Schiller,
Limited proteolysis of cytokeratin a by an endogeneous protease: removal of positively charged terminal sequences.
1983,
Pubmed
Schmidt,
Identification of cytokeratin antigens in Novikoff ascites hepatoma.
1982,
Pubmed
Schweizer,
Synthesis in vitro of keratin polypeptides directed by mRNA isolated from newborn and adult mouse epidermis.
1980,
Pubmed
Sharp,
Occurrence of two different intermediate filament proteins in the same filament in situ within a human glioma cell line. An immunoelectron microscopical study.
1982,
Pubmed
Steinert,
Heterogeneity of intermediate filaments assembled in vitro.
1982,
Pubmed
Sun,
Keratin cytoskeletons in epithelial cells of internal organs.
1979,
Pubmed
Sun,
Immunofluorescent staining of keratin fibers in cultured cells.
1978,
Pubmed
Wu,
A new small (40 kd) keratin filament protein made by some cultured human squamous cell carcinomas.
1981,
Pubmed
Zehner,
Characterization of the chicken vimentin gene: single copy gene producing multiple mRNAs.
1983,
Pubmed
