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Dev Biol
2008 May 19;1210:1-10. doi: 10.1016/j.brainres.2008.03.017.
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Protein kinase C isozyme-specific potentiation of expressed Ca v 2.3 currents by acetyl-beta-methylcholine and phorbol-12-myristate, 13-acetate.
Rajagopal S
,
Fang H
,
Patanavanich S
,
Sando JJ
,
Kamatchi GL
.
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Protein kinase C (PKC) is implicated in the potentiation of Ca v 2.3 currents by acetyl-beta-methylcholine (MCh), a muscarinic M1 receptor agonist or phorbol-12-myristate, 13-acetate (PMA). The PKC isozymes responsible for the action of MCh and PMA were investigated using translocation as a measure of activation and with isozyme-selective antagonists and siRNA. Ca v channels were expressed with alpha1 2.3, beta1b and alpha2delta subunits and muscarinic M1 receptors in the Xenopus oocytes and the expressed currents (I Ba) were studied using Ba2+ as the charge carrier. Translocation of PKC isozymes to the membrane studied by Western blot revealed that all eleven known PKC isozymes are present in the Xenopus oocytes. Exposure of the oocytes to MCh led to the translocation of PKC alpha whereas PMA activated PKC betaII and epsilon isozymes. The action of MCh was inhibited by Go 6976, an inhibitor of cPKC isozymes or PKC alpha siRNA. PMA-induced potentiation of Ca v 2.3 currents was inhibited by CG533 53, a PKC betaII antagonist, betaIIV5.3, a peptide translocation inhibitor of PKC betaII or PKC betaII siRNA. Similarly, epsilonV1.2, a peptide translocation inhibitor of PKC epsilon or PKC epsilon siRNA inhibited PMA action. The inhibitors of PKC increased the basal I Ba slightly. It is possible that some PKC isozymes have negative control over the I Ba. Our results implicate PKC alpha in the potentiation of Ca v 2.3 currents by MCh and PKC betaII and epsilon in the potentiation of Ca v 2.3 currents by PMA.
Benussi,
Specific role for protein kinase C alpha in the constitutive and regulated secretion of amyloid precursor protein in human skin fibroblasts.
1998, Pubmed
Benussi,
Specific role for protein kinase C alpha in the constitutive and regulated secretion of amyloid precursor protein in human skin fibroblasts.
1998,
Pubmed
Castellano,
Cloning and expression of a third calcium channel beta subunit.
1993,
Pubmed
,
Xenbase
Caulfield,
Muscarinic receptors--characterization, coupling and function.
1993,
Pubmed
Chalfant,
A carboxy-terminal deletion mutant of protein kinase C beta II inhibits insulin-stimulated 2-deoxyglucose uptake in L6 rat skeletal muscle cells.
1996,
Pubmed
Corbalán-García,
Protein kinase C regulatory domains: the art of decoding many different signals in membranes.
2006,
Pubmed
Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987,
Pubmed
,
Xenbase
Dolphin,
A short history of voltage-gated calcium channels.
2006,
Pubmed
Elmslie,
Neurotransmitter modulation of neuronal calcium channels.
2003,
Pubmed
Ertel,
Nomenclature of voltage-gated calcium channels.
2000,
Pubmed
Evans,
Specific translocation of protein kinase Calpha to the plasma membrane requires both Ca2+ and PIP2 recognition by its C2 domain.
2006,
Pubmed
Fang,
Role of alpha1 2.3 subunit I-II linker sites in the enhancement of Ca(v) 2.3 current by phorbol 12-myristate 13-acetate and acetyl-beta-methylcholine.
2005,
Pubmed
,
Xenbase
Fang,
Inhibitory role of Ser-425 of the alpha1 2.2 subunit in the enhancement of Cav 2.2 currents by phorbol-12-myristate, 13-acetate.
2006,
Pubmed
,
Xenbase
Gallegos,
Targeting protein kinase C activity reporter to discrete intracellular regions reveals spatiotemporal differences in agonist-dependent signaling.
2006,
Pubmed
Hamid,
Identification of an integration center for cross-talk between protein kinase C and G protein modulation of N-type calcium channels.
1999,
Pubmed
Johnson,
Progesterone acts through protein kinase C to remodel the cytoplasm as the amphibian oocyte becomes the fertilization-competent egg.
1997,
Pubmed
,
Xenbase
Kamatchi,
Distinct regulation of expressed calcium channels 2.3 in Xenopus oocytes by direct or indirect activation of protein kinase C.
2003,
Pubmed
,
Xenbase
Kamatchi,
Identification of sites responsible for potentiation of type 2.3 calcium currents by acetyl-beta-methylcholine.
2004,
Pubmed
,
Xenbase
Leno,
DNA replication in cell-free extracts from Xenopus laevis.
1991,
Pubmed
,
Xenbase
Lenz,
Ca2+-controlled competitive diacylglycerol binding of protein kinase C isoenzymes in living cells.
2002,
Pubmed
McHugh,
Inhibition of cardiac L-type calcium channels by protein kinase C phosphorylation of two sites in the N-terminal domain.
2000,
Pubmed
Melliti,
Muscarinic stimulation of alpha1E Ca channels is selectively blocked by the effector antagonist function of RGS2 and phospholipase C-beta1.
2000,
Pubmed
Nishizuka,
The heterogeneity and differential expression of multiple species of the protein kinase C family.
1988,
Pubmed
Nishizuka,
Protein kinase C and lipid signaling for sustained cellular responses.
1995,
Pubmed
Oancea,
Protein kinase C as a molecular machine for decoding calcium and diacylglycerol signals.
1998,
Pubmed
Pemberton,
Inhibition of the L-type calcium channel by the five muscarinic receptors (m1-m5) expressed in NIH 3T3 cells.
1997,
Pubmed
Rane,
Kinase C activator 1,2-oleoylacetylglycerol attenuates voltage-dependent calcium current in sensory neurons.
1986,
Pubmed
Snutch,
The use of Xenopus oocytes to probe synaptic communication.
1988,
Pubmed
,
Xenbase
Stith,
sn-1,2-diacylglycerol and choline increase after fertilization in Xenopus laevis.
1997,
Pubmed
,
Xenbase
Stumpo,
Protein kinase C isozyme distribution and down-regulation in relation to insulin-stimulated c-fos induction.
1994,
Pubmed
Swartz,
Modulation of Ca2+ channels by protein kinase C in rat central and peripheral neurons: disruption of G protein-mediated inhibition.
1993,
Pubmed
Vijayaragavan,
Modulation of Nav1.7 and Nav1.8 peripheral nerve sodium channels by protein kinase A and protein kinase C.
2004,
Pubmed
,
Xenbase
Walker,
Subunit interaction sites in voltage-dependent Ca2+ channels: role in channel function.
1998,
Pubmed
Wetsel,
Tissue and cellular distribution of the extended family of protein kinase C isoenzymes.
1992,
Pubmed
Yang,
Enhancement of N- and L-type calcium channel currents by protein kinase C in frog sympathetic neurons.
1993,
Pubmed
Zühlke,
Ca2+-sensitive inactivation of L-type Ca2+ channels depends on multiple cytoplasmic amino acid sequences of the alpha1C subunit.
1998,
Pubmed
,
Xenbase
