Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Proc Natl Acad Sci U S A
1994 Jun 21;9113:6069-73.
Show Gene links
Show Anatomy links
Cloning and functional expression of a Drosophila gamma-aminobutyric acid receptor.
Chen R
,
Belelli D
,
Lambert JJ
,
Peters JA
,
Reyes A
,
Lan NC
.
???displayArticle.abstract???
A cDNA encoding a functional gamma-aminobutyric (GABA)-activated Cl- channel has been isolated from an adult Drosophila head cDNA library. When expressed in Xenopus laevis oocytes, the subunit functions efficiently, presumably as a homooligomeric complex and is activated by GABA or muscimol. GABA-evoked currents are highly sensitive to antagonism by picrotoxin but are insensitive to bicuculline, RU 5135, or zinc. Pentobarbitone greatly enhances GABA-evoked currents, whereas the neurosteroid 5 alpha-pregnan-3 alpha-ol-20-one demonstrates a large reduction in both the potency and maximal effect when compared with its actions upon vertebrate GABA type A receptors. Although zinc-insensitive, the subunit is also insensitive to flunitrazepam. Hence, the GABA receptors formed by this subunit exhibit a unique pharmacology when compared with vertebrate GABA type A receptors or those composed of rho subunits. Because the receptor-channel complex functions as a homooligomer, this subunit may be of value in mutagenesis studies aiming to define drug-binding sites.
Blair,
Single subunits of the GABAA receptor form ion channels with properties of the native receptor.
1988, Pubmed,
Xenbase
Blair,
Single subunits of the GABAA receptor form ion channels with properties of the native receptor.
1988,
Pubmed
,
Xenbase
Bormann,
Electrophysiology of GABAA and GABAB receptor subtypes.
1988,
Pubmed
Darlison,
Invertebrate GABA and glutamate receptors: molecular biology reveals predictable structures but some unusual pharmacologies.
1992,
Pubmed
Draguhn,
Functional and molecular distinction between recombinant rat GABAA receptor subtypes by Zn2+.
1990,
Pubmed
ffrench-Constant,
Drosophila gamma-aminobutyric acid receptor gene Rdl shows extensive alternative splicing.
1993,
Pubmed
Ffrench-Constant,
A point mutation in a Drosophila GABA receptor confers insecticide resistance.
1993,
Pubmed
,
Xenbase
Ffrench-Constant,
Molecular cloning and transformation of cyclodiene resistance in Drosophila: an invertebrate gamma-aminobutyric acid subtype A receptor locus.
1991,
Pubmed
Harvey,
Sequence of a functional invertebrate GABAA receptor subunit which can form a chimeric receptor with a vertebrate alpha subunit.
1991,
Pubmed
,
Xenbase
Henderson,
Characterization of a putative gamma-aminobutyric acid (GABA) receptor beta subunit gene from Drosophila melanogaster.
1993,
Pubmed
Hope,
Cloning and functional expression of an apparent splice variant of the murine 5-HT3 receptor A subunit.
1993,
Pubmed
,
Xenbase
Hutton,
A novel invertebrate GABAA receptor-like polypeptide. Sequence and pattern of gene expression.
1993,
Pubmed
,
Xenbase
Nakahiro,
General anesthetics modulate GABA receptor channel complex in rat dorsal root ganglion neurons.
1989,
Pubmed
Nash,
New mutants of Drosophila that are resistant to the anesthetic effects of halothane.
1991,
Pubmed
Newland,
On the mechanism of action of picrotoxin on GABA receptor channels in dissociated sympathetic neurones of the rat.
1992,
Pubmed
Palazzolo,
Phage lambda cDNA cloning vectors for subtractive hybridization, fusion-protein synthesis and Cre-loxP automatic plasmid subcloning.
1990,
Pubmed
Peters,
Modulation of the GABAA receptor by depressant barbiturates and pregnane steroids.
1988,
Pubmed
Puia,
Neurosteroids act on recombinant human GABAA receptors.
1990,
Pubmed
Sanger,
DNA sequencing with chain-terminating inhibitors.
1977,
Pubmed
Shimada,
gamma-Aminobutyric acid A or C receptor? gamma-Aminobutyric acid rho 1 receptor RNA induces bicuculline-, barbiturate-, and benzodiazepine-insensitive gamma-aminobutyric acid responses in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Sieghart,
GABAA receptors: ligand-gated Cl- ion channels modulated by multiple drug-binding sites.
1992,
Pubmed
Sigel,
Point mutations affecting antagonist affinity and agonist dependent gating of GABAA receptor channels.
1992,
Pubmed
,
Xenbase
Sigel,
The effect of subunit composition of rat brain GABAA receptors on channel function.
1990,
Pubmed
,
Xenbase
Simmonds,
Antagonism of inhibitory amino acids by the steroid derivative RU5135.
1985,
Pubmed
Smart,
A novel effect of zinc on the lobster muscle GABA receptor.
1982,
Pubmed
Thompson,
Cloning and sequencing of the cyclodiene insecticide resistance gene from the yellow fever mosquito Aedes aegypti. Conservation of the gene and resistance associated mutation with Drosophila.
1993,
Pubmed
Yanisch-Perron,
Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
1985,
Pubmed
Zaman,
Unusual effects of benzodiazepines and cyclodiene insecticides on an expressed invertebrate GABAA receptor.
1992,
Pubmed
