Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
XB-ART-45744
FEBS Lett 2012 Jun 04;58611:1638-44. doi: 10.1016/j.febslet.2012.04.035.
Show Gene links Show Anatomy links

Live imaging of active fluorophore labelled Wnt proteins.

Holzer T , Liffers K , Rahm K , Trageser B , Ozbek S , Gradl D .


???displayArticle.abstract???
For almost 30 years, Wnt proteins have been known as key regulators of many developmental decisions, including the formation of the embryonic axes, patterning of the CNS, limb bud outgrowth and segment polarity. However, live cell imaging of active Wnt proteins was rarely reported. Here, we have generated a Wnt2b-EGFP fusion protein that retains functionality in bona fide Wnt activity assays, although the secreted protein is rapidly cleaved by extracellular proteases. We can show with this new tool that Wnt2b-EGFP moves along the microtubules of Wnt producing cells and that this directed movement is essential for the secretion of active Wnt protein.

???displayArticle.pubmedLink??? 22554900
???displayArticle.link??? FEBS Lett


Species referenced: Xenopus
Genes referenced: wnt2b