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Mol Cell Biol
2005 Mar 01;255:1764-78. doi: 10.1128/MCB.25.5.1764-1778.2005.
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Modulation of histone deposition by the karyopherin kap114.
Mosammaparast N
,
Del Rosario BC
,
Pemberton LF
.
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The nuclear import of histones is a prerequisite for the downstream deposition of histones to form chromatin. However, the coordinate regulation of these processes remains poorly understood. Here we demonstrate that Kap114p, the primary karyopherin/importin responsible for the nuclear import of histones H2A and H2B, modulates the deposition of histones H2A and H2B by the histone chaperone Nap1p. We show that a complex comprising Kap114p, histones H2A and H2B, and Nap1p is present in the nucleus and that the presence of this complex is specifically promoted by Nap1p. This places Kap114p in a position to modulate Nap1p function, and we demonstrate by the use of two different assay systems that Kap114p inhibits Nap1p-mediated chromatin assembly. The inhibition of H2A and H2B deposition by Kap114p results in the concomitant inhibition of RCC1 loading onto chromatin. Biochemical evidence suggests that the mechanism by which Kap114p modulates histone deposition primarily involves direct histone binding, while the interaction between Kap114p and Nap1p plays a secondary role. Furthermore, we found that the inhibition of histone deposition by Kap114p is partially reversed by RanGTP. Our results indicate a novel mechanism by which cells can regulate histone deposition and establish a coordinate link between histone nuclear import and chromatin assembly.
Aitchison,
Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins.
1996, Pubmed
Aitchison,
Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins.
1996,
Pubmed
Belotserkovskaya,
Facts about FACT and transcript elongation through chromatin.
2004,
Pubmed
Bilbao-Cortés,
Ran binds to chromatin by two distinct mechanisms.
2002,
Pubmed
,
Xenbase
Casolari,
Genome-wide localization of the nuclear transport machinery couples transcriptional status and nuclear organization.
2004,
Pubmed
Chang,
Histones in transit: cytosolic histone complexes and diacetylation of H4 during nucleosome assembly in human cells.
1997,
Pubmed
Dasso,
A mutant form of the Ran/TC4 protein disrupts nuclear function in Xenopus laevis egg extracts by inhibiting the RCC1 protein, a regulator of chromosome condensation.
1994,
Pubmed
,
Xenbase
Dasso,
Running on Ran: nuclear transport and the mitotic spindle.
2001,
Pubmed
Dominski,
A 3' exonuclease that specifically interacts with the 3' end of histone mRNA.
2003,
Pubmed
Finley,
The yeast polyubiquitin gene is essential for resistance to high temperatures, starvation, and other stresses.
1987,
Pubmed
Goldstein,
Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae.
1999,
Pubmed
Gontijo,
Repairing DNA damage in chromatin.
2003,
Pubmed
Gunjan,
A Rad53 kinase-dependent surveillance mechanism that regulates histone protein levels in S. cerevisiae.
2003,
Pubmed
Harel,
Importin beta negatively regulates nuclear membrane fusion and nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase
Haushalter,
Chromatin assembly by DNA-translocating motors.
2003,
Pubmed
Hetzer,
GTP hydrolysis by Ran is required for nuclear envelope assembly.
2000,
Pubmed
,
Xenbase
Ishimi,
Identification and molecular cloning of yeast homolog of nucleosome assembly protein I which facilitates nucleosome assembly in vitro.
1991,
Pubmed
,
Xenbase
Ito,
Nucleosome assembly and remodeling.
2003,
Pubmed
,
Xenbase
Ito,
ATP-facilitated chromatin assembly with a nucleoplasmin-like protein from Drosophila melanogaster.
1996,
Pubmed
,
Xenbase
Ito,
Chromatin assembly factors: a dual function in nucleosome formation and mobilization?
1997,
Pubmed
Lee,
Kap104p-mediated nuclear import. Nuclear localization signals in mRNA-binding proteins and the role of Ran and Rna.
1999,
Pubmed
Lounsbury,
Mutations within the Ran/TC4 GTPase. Effects on regulatory factor interactions and subcellular localization.
1996,
Pubmed
Ma,
Deposition-related sites K5/K12 in histone H4 are not required for nucleosome deposition in yeast.
1998,
Pubmed
Mosammaparast,
Pathways mediating the nuclear import of histones H3 and H4 in yeast.
2002,
Pubmed
Mosammaparast,
Nuclear import of histone H2A and H2B is mediated by a network of karyopherins.
2001,
Pubmed
Mosammaparast,
A role for nucleosome assembly protein 1 in the nuclear transport of histones H2A and H2B.
2002,
Pubmed
Nemergut,
Chromatin docking and exchange activity enhancement of RCC1 by histones H2A and H2B.
2001,
Pubmed
,
Xenbase
Pemberton,
A distinct and parallel pathway for the nuclear import of an mRNA-binding protein.
1997,
Pubmed
Pemberton,
Nuclear import of the TATA-binding protein: mediation by the karyopherin Kap114p and a possible mechanism for intranuclear targeting.
1999,
Pubmed
Philpott,
Nucleoplasmin remodels sperm chromatin in Xenopus egg extracts.
1992,
Pubmed
,
Xenbase
Philpott,
Sperm decondensation in Xenopus egg cytoplasm is mediated by nucleoplasmin.
1991,
Pubmed
,
Xenbase
Schlenstedt,
Yrb4p, a yeast ran-GTP-binding protein involved in import of ribosomal protein L25 into the nucleus.
1997,
Pubmed
Senger,
Mtr10p functions as a nuclear import receptor for the mRNA-binding protein Npl3p.
1998,
Pubmed
Sobel,
Conservation of deposition-related acetylation sites in newly synthesized histones H3 and H4.
1995,
Pubmed
Sutton,
Yeast ASF1 protein is required for cell cycle regulation of histone gene transcription.
2001,
Pubmed
Tyler,
The RCAF complex mediates chromatin assembly during DNA replication and repair.
1999,
Pubmed
Tyler,
Chromatin assembly. Cooperation between histone chaperones and ATP-dependent nucleosome remodeling machines.
2002,
Pubmed
Verreault,
Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4.
1996,
Pubmed
Walther,
RanGTP mediates nuclear pore complex assembly.
2003,
Pubmed
,
Xenbase
Weis,
Regulating access to the genome: nucleocytoplasmic transport throughout the cell cycle.
2003,
Pubmed
