Nestor-Bergmann A , Stooke-Vaughan GA , Goddard GK , Starborg T , Jensen OE , Woolner S .

Wellcome Trust , Biotechnology and Biological Sciences Research Council , 098390/Z/12/Z Wellcome Trust , 105610/Z/14/Z Wellcome Trust

	Figure 1. Application of Tensile Force to a Multi-layered Tissue(A) Animal cap tissue was dissected from stage 10 Xenopus laevis embryos and adhered to fibronectin-coated PDMS membranes, and a 35% uniaxial stretch of the membrane was applied.(B) 3View scanning electron micrograph showing that the cultured animal cap tissue is two to three cells thick. Cell shape and divisions were assessed in the apical cell layer.(C) Displacement of nuclei was tracked in a stretched animal cap.(D) Confocal images of the apical cells in unstretched and stretched animal caps (green, GFP-alpha-tubulin; magenta, cherry-histone2B), taken 0 and 90 min after stretch. Representative cells outlined by dashed lines.(E) Rose plot showing orientation of cell shape relative to direction of stretch in unstretched (blue) and stretched (red; measured immediately following stretch) experiments.(F) Cumulative plots of cell circularity in unstretched (blue) and stretched (red; at 0, 30, 60 and 90 min after stretch) animal caps (0 = straight line, 1 = circle). One hundred percent of cells have circularity ≤ 1. Markers are slightly offset for clarity. Error bars represent 95% confidence intervals.(G) Rose plot of division angle relative to direction of stretch for unstretched (blue) and stretched (red) experiments. Kolmogorov-Smirnov test indicates that the unstretched distribution is not significantly different from a uniform distribution, n = 343 divisions, 15 animal caps; Kolmogorov-Smirnov test indicates that stretched distribution is significantly different from uniform, p < 1.4 × 10−9, n = 552 divisions, 17 animal caps.Scale bars, 10 μm in (B), 500 μm in (C), and 50 μm in (D).
	Figure 2. Cell Division Orientation Is Best Predicted by an Axis of Shape Defined by TCJs(A) Representative image of control cells from an unstretched experiment. Scale bar, 20 μm.(A′) Overlay of segmentation of cells given in (A), with the principal axis of shape characterized by area, perimeter, and junctions drawn in red, blue, and yellow, respectively.(A″) Enlargement of segmented cells from white box drawn in (A′); cells analyzed are outlined by dashed white line.(B) Circularities of 2,035 cells from unstretched experiments, with shape characterized by area, perimeter, and junctions plotted in red, blue, and yellow respectively. Cells have been ordered in descending order of perimeter-based circularity (CP), with the corresponding values of CA and CJ plotted alongside.(C) Rose plot of difference between division angle, θD, and orientation of shape on the basis of perimeter (blue; θshape=θP) and junctions (yellow; θshape=θJ), for cells that satisfy |θP−θJ|≥15°.(D) Rose plot of difference between division angle, θD, and orientation of shape on the basis of area (red; θshape=θA) and junctions (yellow; θshape=θJ), for cells that satisfy |θA−θJ|≥15°.(E) Examples of elongated (top) and round (bottom) cells where division angle (black arrows) is well predicted by the principal axis of shape defined by area (yellow arrows).(F) Rose plot of difference between division angle, θD, and orientation of shape on the basis of perimeter (blue; θshape=θP) and junctions (yellow; θshape=θJ), for round cells that satisfy CA > 0.65.(G) Rose plot of difference between division angle, θD, and orientation of shape on the basis of area (red; θshape=θA) and junctions (yellow; θshape=θJ), for round cells that satisfy CA > 0.65. See also Figure S1.(H) Rose plot of difference between division angle, θD, and orientation of shape on the basis of Minc model when β = 3 (magenta; θshape=θMinc) and junctions (yellow; θshape=θJ) for all cells in stretched and unstretched experiments (n = 599 cells).(I) Rose plot of difference between division angle, θD, and orientation of shape on the basis of Minc model when β = 3 (magenta; θshape=θMinc) and junctions (yellow; θshape=θJ), for cells that satisfy |θMinc−θJ|≥15° (n = 65 cells).(J) Cumulative plot of difference between division angle, θD, and orientation of shape for data shown in (I).
	Figure 3. Division Orientation Is Better Predicted by Shape Rather Than High Relative Isotropic or Shear Stress(A) Images taken from a confocal time-lapse video of a division in a cell in stretched tissue whose interphase shape (dashed line, 0:00) is oriented with the stretch (horizontal) axis. Cell division aligns with both cell shape and stretch axis.(B) Time-lapse images of an unusual cell in a stretched tissue, whose interphase shape (dashed line, 0:00) is oriented against the stretch axis. Cell division aligns with cell shape but against the stretch axis.(C) Rose plot of difference between division angle, θD, and orientation of shape on the basis of junctions, θJ, for cells from stretched experiments, where θJ was at least 60° divergent to the direction of stretch. Twenty-nine cells satisfied this condition. Kolmogorov-Smirnov test found a significant difference from a uniform distribution (p = 0.022).(D) Representative cells showing classification of cell stress configurations. Red (blue) cells are under net tension (compression), where Peff is positive (negative). Larger (smaller) black arrows indicate the orientation of the principal (secondary) axis of stress, with inward- (outward)-pointing arrows indicating the tension (compression) generated by the cell. Yellow arrows indicate the principal axis of shape defined by cell junctions, which aligns exactly with a principal axis of stress.(E) Fifty simulated cells randomly generated in a periodic box, relaxed to equilibrium with parameters (Λ,Γ) = (−0.259, 0.172), under conditions of zero global stress (Nestor-Bergmann et al., 2018a). Red (blue) cells are under net tension (compression). Principal axis of stress (shape) indicated in black (yellow).(F) Cells from (E) following a 13% area-preserving uniaxial stretch along the x axis.(G) Example segmented cells from an unstretched experiment. Cells in red (blue) are predicted to be under net tension (compression).(H) Cell circularity defined by junctions, CJ, versus |θD−θJ|. Spearman rank correlation coefficient found a significant correlation (p < 3.04 × 10−10). Elongated cells (CJ ≤ 0.65) cluster in blue box, whereas rounded cells (CJ > 0.65) have a more uniform distribution.(I) Rose plot of difference between division angle, θD, and orientation of shape on the basis of junctions, θJ for round (CJ > 0.65; right) and elongated (CJ ≤ 0.65; left) cells shown in (H). Mann-Whitney U test indicated that elongated cells have θJ aligned significantly more with θD than rounded cells (p < 1.64 × 10−8).Scale bars in (A) and (B), 20 μm. All rose plots show percentage of cells.See also Figure S2.
	Figure 4. C-Cadherin Is Required to Orient the Mitotic Spindle According to Cell Shape(A) Single confocal slices from immunofluorescent staining for β-catenin (green) and myc-tag (magenta) in uninjected and CdhFL-injected stage 12 embryos (stage matched to time that animal caps are stretched and imaged). Hotspots of β-catenin localization (arrows) are seen at TCJs in controls but are lost when CdhFL is overexpressed.(B) Schematic of Cadherin constructs CdhFL and CdhΔC.(C) Rose plot of division angles, θD, relative to direction of stretch for cells from stretched CdhΔC-injected (411 cells; cyan) and stretched CdhFL-injected experiments (552 cells; orange). CdhFL-injected cells align significantly better with direction of stretch (p < 0.0162, Mann-Whitney U test).(D) Rose plot of difference between division angle, θD, and orientation of shape on the basis of junctions, θJ, for cells from CdhΔC-injected experiments (390 cells; cyan) and control experiments (239 cells; blue). Distributions are significantly different (p < 0.016 Kolmogorov-Smirnov test).(E) Rose plot of difference between division angle, θD, and orientation of shape on the basis of perimeter, θP, (blue) and junctions, θJ, (yellow) for 96 cells from CdhFL-injected experiments that satisfied |θP−θJ|≥15°. θD aligns significantly better to θP than a random distribution (p < 0.004; Kolmogorov-Smirnov test), but not to θJ.(F) Images from time-lapse videos of control and CdhFL-injected animal cap tissue expressing GFP-LGN in a mosaic fashion. In control cells, GFP-LGN is enriched at TCJs during interphase (arrows), and this localization persists through mitosis. The enrichment of GFP-LGN at TCJs is lost when CdhFL is expressed, with localization spread throughout the cell edge (line).(G) Quantification of GFP-LGN localization at TCJs compared with cell edges in single mitotic cells in animal caps. GFP-LGN is more strongly localized at TCJs compared with cells edges in controls, but this bias is lost in CdhFL-injected tissue (∗p < 0.05, Kolmogorov-Smirnov test; n = 21 and 23 mitotic cells from seven and six unrelated animal caps for control and CdhFL, respectively). Error bars represent mean and SD.Red points show quantification for mitotic cells highlighted in (F). Rose plots show percentage of cells. Scale bars, 20 μm.See also Figure S3.
	Figure 5. Stretching Increases Division RateDividing cells have large area, perimeter, and relative effective pressure.(A) Division rate (percentage of cells entering mitosis per hour) increases in stretched tissue compared with unstretched. Ninety-five percent confidence intervals do not overlap, indicating significant difference. Each point represents the mean division rate from an animal cap.(B) Percentage of cells that have undergone nuclear envelope breakdown (NEB) with respect to time in control stretched (red) and unstretched (blue) experiments from (A). Dashed lines indicate linear lines of best fit; control unstretched experiments have gradient 4.2% cells undergoing division per hour. Stretched experiments have initial gradient 8.1% and then 4.35% cells undergoing division per hour.(C) Comparison of mean area of population of all cells versus dividing cells from unstretched and stretched control experiments. Error bars represent mean and 95% confidence intervals, which do not overlap between the population and dividing cells, indicating a significant difference.(D) Comparison of mean perimeter of population of all cells versus dividing cells from unstretched and stretched control experiments. Error bars represent mean and 95% confidence intervals, which do not overlap between the population and dividing cells, indicating a significant difference.(E) Heatmap showing predicted relative isotropic stress (effective pressure, Peff) of dividing cells from control unstretched experiments. Areas and perimeters have been nondimensionalized using the preferred areas, A˜0, fitted to each experiment in Figure S4C. Polygonal class (number of neighbors) indicated by marker color and style, with (4, 5, 6, 7, 8+) sided cells given in (blue, green, red, purple, yellow). Dashed vertical line represents mean area of all cells. Cells lying in red (blue) regions are under predicted net tension (compression).
	Figure 6. Myosin II MO Cells Maintain Alignment of Division to TCJ Shape, but Have Perturbed Proliferation Rate(A) Images taken from a confocal time-lapse video of stretched myosin II morpholino-injected animal cap explants at 0 and 90 min intervals. Butterfly nuclei seen prominently at 90 min, where nuclei are in contact.(B) Time-lapse images of control morpholino-injected stretched animal cap explants at 0 and 90 min intervals.(C) Cumulative distribution of cell circularity defined by area, CA, in myosin II MO knockdown stretched animal caps (shaded green) at t = 0, 30, 60, and 90 min after stretch. Cumulative distribution for unstretched t = 0 control MO knockdown experiments shown in blue. Error bars represent 95% confidence intervals. Error bars for myosin II MO t = 90 min distribution does not overlap with control MO, indicating a significant difference from unstretched shape. Markers are slightly offset for clarity.(D) Rose plot of difference between division angle, θD, and orientation of shape on the basis of junctions, θJ, for 216 cells from myosin II knockdown stretched experiments. Mann-Whitney U test found significant alignment compared with random (p < 5.72 × 10−15) but no significant difference from equivalent dataset in control stretched experiments. Percentages of cells shown.(E) Rose plot of division angle relative to direction for stretch for control MO (532 cells; blue) and myosin II MO (301 cells; green) experiments. Mann-Whitney U and Kolmogorov-Smirnov tests found no significant difference between the two.(F) Division rate (percentage of total cells entering mitosis per hour) in unstretched and stretched tissue from myosin II MO (green; n = 10 for unstretched and n = 12 for stretched) and control MO (blue; n = 13 for unstretched and n = 10 for stretched) experiments. Error bars represent mean and 95% confidence intervals.(G) Comparison of mean area of population of all cells versus dividing cells from stretched myosin II knockdown experiments. Error bars represent mean and 95% confidence intervals, which overlap, indicating no significant difference.(H) Comparison of mean perimeter of population of all cells versus dividing cells from stretched myosin II knockdown experiments. Error bars represent mean and 95% confidence intervals, which overlap, indicating no significant difference.Scale bars in (A) and (B), 100 μm.

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