Graindorge A , Thuret R , Pollet N , Osborne HB , Audic Y .

	Figure 1. Comparison of microarray and qRT–PCR results. Relative amounts of poly(A)+ mRNA of the indicated gene products in stage VI oocytes (black bars), UFE (gray bars) and 64-cell embryos (open bars), as measured by (A) microarrays and (B) qRT–PCR. Relative levels for microarray data are given with the maximal normalized intensity as 100%, and for qRT–PCR, the maximal poly(A)+ mRNA amount is considered as 100%.
	Figure 2. Sequence analysis of mRNAs classified according to their adenylation behaviors. Regulatory sequences were searched for in putative 3′-UTR of mRNAs in each category. For each category, the percentage of sequences containing the regulatory element is indicated. The number of sequences analyzed and the total length of the sequence in each category are indicated. The percentage of each regulatory sequence elements in the totality of the 3′-UTR sequences of categorized mRNAs (cat UTR) and of all the UTR's recovered from the genome annotation (whole 3′-UTR) is indicated.
	Figure 3. RL-PAT specificity. (A) Schematic of the RNA ligation mediated poly(A) test, as described by Rassa et al. (35). P1 denotes the primer ligated to the 3′-UTR of the RNA, the 3′ end of P1 is blocked by an amino group. cDNA synthesis is achieved from primer P′1 and PCR products are radiolabeled by the 32P phosphorylated gene-specific primer (GSP). X indicates a restriction site, radiolabeled fragments are in gray. (B) Specificity of RL-PAT was tested by restriction digest (D) of PCR products obtained from StVI oocyte or UFE RNA as indicated (St., stage). The identity of the tested mRNAs is indicated above each lane. When indicated, RNase H (RH) digestion in the presence of oligo(dT) was performed prior to the RL-PAT. M, radiolabeled DNA size markers as indicated (base pairs).
	Figure 4. Adenylation behaviors of selected X.tropicalis endogenous mRNAs. Total RNAs from StVI oocytes, UFE and 64C embryos were analyzed by RL-PAT. The identity of the tested mRNAs is indicated above each panel. Oligo(dT)/RNase H digestion (RH) was performed on StVI oocyte total RNA. M, DNA size markers. In RT- control lanes, ligated RNAs were not reverse transcribed before PCR. (A) RL-PAT results for mRNAs with known adenylation behaviors in X.laevis. (B) RL-PAT results for mRNAs with unknown behaviors.

Audic, Zygotic regulation of maternal cyclin A1 and B2 mRNAs. 2001, Pubmed, Xenbase

Audic, Zygotic regulation of maternal cyclin A1 and B2 mRNAs. 2001, Pubmed , Xenbase
Audic, Post-transcriptional regulation in cancer. 2004, Pubmed
Audic, Embryo deadenylation element-dependent deadenylation is enhanced by a cis element containing AUU repeats. 1998, Pubmed , Xenbase
Audic, Postfertilization deadenylation of mRNAs in Xenopus laevis embryos is sufficient to cause their degradation at the blastula stage. 1997, Pubmed , Xenbase
Audic, Zygotic control of maternal cyclin A1 translation and mRNA stability. 2002, Pubmed , Xenbase
Bassez, Post-transcriptional regulation of ornithine decarboxylase in Xenopus laevis oocytes. 1990, Pubmed , Xenbase
Beaudoing, Patterns of variant polyadenylation signal usage in human genes. 2000, Pubmed
Blot, Cell cycle regulation of pEg3, a new Xenopus protein kinase of the KIN1/PAR-1/MARK family. 2002, Pubmed , Xenbase
Bodart, Characterization of MPF and MAPK activities during meiotic maturation of Xenopus tropicalis oocytes. 2002, Pubmed , Xenbase
Bouvet, The deadenylation conferred by the 3' untranslated region of a developmentally controlled mRNA in Xenopus embryos is switched to polyadenylation by deletion of a short sequence element. 1994, Pubmed , Xenbase
Brown, Endonucleolytic cleavage of a maternal homeo box mRNA in Xenopus oocytes. 1990, Pubmed , Xenbase
Brown, Sequence-specific endonucleolytic cleavage and protection of mRNA in Xenopus and Drosophila. 1993, Pubmed , Xenbase
Charlesworth, Cytoplasmic polyadenylation element (CPE)- and CPE-binding protein (CPEB)-independent mechanisms regulate early class maternal mRNA translational activation in Xenopus oocytes. 2004, Pubmed , Xenbase
Charlesworth, A novel regulatory element determines the timing of Mos mRNA translation during Xenopus oocyte maturation. 2002, Pubmed , Xenbase
Cubizolles, pEg7, a new Xenopus protein required for mitotic chromosome condensation in egg extracts. 1998, Pubmed , Xenbase
Dallas, Gene expression levels assessed by oligonucleotide microarray analysis and quantitative real-time RT-PCR -- how well do they correlate? 2005, Pubmed
de Moor, Mechanisms of translational control by the 3' UTR in development and differentiation. 2005, Pubmed
Du, Activity-dependent polyadenylation in neurons. 2005, Pubmed , Xenbase
Duncan, The poly(A)(+)RNA sequence complexity is also represented in poly(A)(-)RNA in sea-urchin embryos. 1984, Pubmed
Dworkin, Changes in RNA titers and polyadenylation during oogenesis and oocyte maturation in Xenopus laevis. 1985, Pubmed , Xenbase
Dworkin, Cellular titers and subcellular distributions of abundant polyadenylate-containing ribonucleic acid species during early development in the frog Xenopus laevis. 1981, Pubmed , Xenbase
Dworkin, Mobilization of specific maternal RNA species into polysomes after fertilization in Xenopus laevis. 1985, Pubmed , Xenbase
Dworkin, Metabolic regulation during early frog development. Identification of proteins labeled by 32P-glycolytic intermediates. 1987, Pubmed , Xenbase
Fox, Poly(A) addition during maturation of frog oocytes: distinct nuclear and cytoplasmic activities and regulation by the sequence UUUUUAU. 1989, Pubmed , Xenbase
Fox, Poly(A) removal during oocyte maturation: a default reaction selectively prevented by specific sequences in the 3' UTR of certain maternal mRNAs. 1990, Pubmed , Xenbase
Fritz, Regulation of the mRNAs encoding proteins of the BMP signaling pathway during the maternal stages of Xenopus development. 2001, Pubmed , Xenbase
Gautier-Courteille, EDEN-BP-dependent post-transcriptional regulation of gene expression in Xenopus somitic segmentation. 2004, Pubmed , Xenbase
Hilgers, In vivo analysis of mRNA stability using the Tet-Off system in the chicken embryo. 2005, Pubmed
Houliston, The kinesin-related protein Eg5 associates with both interphase and spindle microtubules during Xenopus early development. 1994, Pubmed , Xenbase
Iwabuchi, Two cellular proteins that bind to wild-type but not mutant p53. 1994, Pubmed
Iwabuchi, Stimulation of p53-mediated transcriptional activation by the p53-binding proteins, 53BP1 and 53BP2. 1998, Pubmed
Le Guellec, Cloning by differential screening of a Xenopus cDNA that encodes a kinesin-related protein. 1991, Pubmed , Xenbase
McGrew, Poly(A) elongation during Xenopus oocyte maturation is required for translational recruitment and is mediated by a short sequence element. 1989, Pubmed , Xenbase
Mendez, Translational control by CPEB: a means to the end. 2001, Pubmed , Xenbase
Murphy, Survivin mRNA is down-regulated during early Xenopus laevis embryogenesis. 2002, Pubmed , Xenbase
Naumovski, The p53-binding protein 53BP2 also interacts with Bc12 and impedes cell cycle progression at G2/M. 1996, Pubmed
Newport, A major developmental transition in early Xenopus embryos: II. Control of the onset of transcription. 1982, Pubmed , Xenbase
Newport, A major developmental transition in early Xenopus embryos: I. characterization and timing of cellular changes at the midblastula stage. 1982, Pubmed , Xenbase
Osborne, Expression and post-transcriptional regulation of ornithine decarboxylase during early Xenopus development. 1991, Pubmed , Xenbase
Paillard, East of EDEN was a poly(A) tail. 2003, Pubmed , Xenbase
Paillard, Identification of a C-rich element as a novel cytoplasmic polyadenylation element in Xenopus embryos. 2000, Pubmed , Xenbase
Paris, Changes in the polyadenylation of specific stable RNA during the early development of Xenopus laevis. 1988, Pubmed , Xenbase
Paris, Poly(A) metabolism and polysomal recruitment of maternal mRNAs during early Xenopus development. 1990, Pubmed , Xenbase
Rassa, Spacing constraints on reinitiation of paramyxovirus transcription: the gene end U tract acts as a spacer to separate gene end from gene start sites. 2000, Pubmed
Reimann, Emi1 regulates the anaphase-promoting complex by a different mechanism than Mad2 proteins. 2001, Pubmed , Xenbase
Reimann, Emi1 is required for cytostatic factor arrest in vertebrate eggs. 2002, Pubmed , Xenbase
Roghi, The Xenopus protein kinase pEg2 associates with the centrosome in a cell cycle-dependent manner, binds to the spindle microtubules and is involved in bipolar mitotic spindle assembly. 1998, Pubmed , Xenbase
Saeed, TM4: a free, open-source system for microarray data management and analysis. 2003, Pubmed
Simon, Further analysis of cytoplasmic polyadenylation in Xenopus embryos and identification of embryonic cytoplasmic polyadenylation element-binding proteins. 1994, Pubmed , Xenbase
Simon, Translational control by poly(A) elongation during Xenopus development: differential repression and enhancement by a novel cytoplasmic polyadenylation element. 1992, Pubmed , Xenbase
Slater, Cell-free cytoplasmic polyadenylation of oogenic RNA. 1979, Pubmed
Stanford, Regulation of the G2/M transition in oocytes of xenopus tropicalis. 2003, Pubmed , Xenbase
Tian, A large-scale analysis of mRNA polyadenylation of human and mouse genes. 2005, Pubmed
Tusher, Significance analysis of microarrays applied to the ionizing radiation response. 2001, Pubmed
Varnum, Deadenylation of maternal mRNAs during Xenopus oocyte maturation does not require specific cis-sequences: a default mechanism for translational control. 1990, Pubmed , Xenbase
Vassalli, Translational control. Awakening dormant mRNAs. 1995, Pubmed
Verrotti, Evolutionary conservation of sequence elements controlling cytoplasmic polyadenylylation. 1996, Pubmed , Xenbase
Voeltz, AUUUA sequences direct mRNA deadenylation uncoupled from decay during Xenopus early development. 1998, Pubmed , Xenbase
Watrin, Expression and functional dynamics of the XCAP-D2 condensin subunit in Xenopus laevis oocytes. 2003, Pubmed , Xenbase
Wells, A role for the cytoplasmic polyadenylation element in NMDA receptor-regulated mRNA translation in neurons. 2001, Pubmed
Wilt, The dynamics of maternal poly(A)-containing mRNA in fertilized sea urchin eggs. 1977, Pubmed
Zubiaga, The nonamer UUAUUUAUU is the key AU-rich sequence motif that mediates mRNA degradation. 1995, Pubmed