XB-ART-55229
Methods Mol Biol
2018 Jan 01;1865:243-250. doi: 10.1007/978-1-4939-8784-9_17.
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Cell Cycle Analysis of the Embryonic Brain of Fluorescent Reporter Xenopus tropicalis by Flow Cytometry.
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Many developmental signals are associated with changes in proliferative response. Also, growing organs and tissues can contain different cellular subpopulations with a defined status in the cell cycle, e.g., quiescent in stem cells, high proliferation in progenitors, cell cycle exit in differentiating cells. This chapter describes a method for isolation of individual cell populations from the Xenopus tadpole brain and determination of their cell cycle status using flow cytometry.
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