Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Havin L
,
Git A
,
Elisha Z
,
Oberman F
,
Yaniv K
,
Schwartz SP
,
Standart N
,
Yisraeli JK
.
???displayArticle.abstract???
Vg1 mRNA translocation to the vegetal cortex of Xenopus oocytes requires intact microtubules, and a 3' UTR cis-acting element (termed VLE), which also mediates sequence-specific binding of several proteins. One protein, the 69-kD Vg1 RBP, associates Vg1 RNA to microtubules in vitro. Here we show that Vg1 RBP-binding sites correlate with vegetal localization. Purification and cloning of Vg1 RBP revealed five RNA-binding motifs: four KH and one RRM domains. Surprisingly, Vg1 RBP is highly homologous to the zipcode binding protein implicated in the microfilament-mediated localization of beta actin mRNA in fibroblasts. These data support Vg1 RBP's direct role in vegetal localization and suggest the existence of a general, evolutionarily conserved mechanism for mRNA targeting.
???displayArticle.pubmedLink???
9620847
???displayArticle.pmcLink???PMC316865 ???displayArticle.link???Genes Dev
Bassell,
Sorting of beta-actin mRNA and protein to neurites and growth cones in culture.
1998, Pubmed
Bassell,
Sorting of beta-actin mRNA and protein to neurites and growth cones in culture.
1998,
Pubmed
Deshler,
Localization of Xenopus Vg1 mRNA by Vera protein and the endoplasmic reticulum.
1997,
Pubmed
,
Xenbase
Elisha,
Vg1 RNA binding protein mediates the association of Vg1 RNA with microtubules in Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Erdélyi,
Requirement for Drosophila cytoplasmic tropomyosin in oskar mRNA localization.
1995,
Pubmed
Forristall,
Patterns of localization and cytoskeletal association of two vegetally localized RNAs, Vg1 and Xcat-2.
1995,
Pubmed
,
Xenbase
Gautreau,
Two copies of a subelement from the Vg1 RNA localization sequence are sufficient to direct vegetal localization in Xenopus oocytes.
1997,
Pubmed
,
Xenbase
Gavis,
Expeditions to the pole: RNA localization in Xenopus and Drosophila.
1997,
Pubmed
,
Xenbase
Kaminski,
Direct evidence that polypyrimidine tract binding protein (PTB) is essential for internal initiation of translation of encephalomyocarditis virus RNA.
1995,
Pubmed
Kislauskis,
Isoform-specific 3'-untranslated sequences sort alpha-cardiac and beta-cytoplasmic actin messenger RNAs to different cytoplasmic compartments.
1993,
Pubmed
Kislauskis,
Sequences responsible for intracellular localization of beta-actin messenger RNA also affect cell phenotype.
1994,
Pubmed
Kloc,
Translocation of repetitive RNA sequences with the germ plasm in Xenopus oocytes.
1993,
Pubmed
,
Xenbase
Kloc,
Two distinct pathways for the localization of RNAs at the vegetal cortex in Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Kuznetsov,
Actin-dependent organelle movement in squid axoplasm.
1992,
Pubmed
Lillie,
Suppression of a myosin defect by a kinesin-related gene.
1992,
Pubmed
Lustig,
Expression cloning of a Xenopus T-related gene (Xombi) involved in mesodermal patterning and blastopore lip formation.
1996,
Pubmed
,
Xenbase
Melton,
Translocation of a localized maternal mRNA to the vegetal pole of Xenopus oocytes.
,
Pubmed
,
Xenbase
Mosquera,
A mRNA localized to the vegetal cortex of Xenopus oocytes encodes a protein with a nanos-like zinc finger domain.
1993,
Pubmed
,
Xenbase
Mowry,
Complex formation between stage-specific oocyte factors and a Xenopus mRNA localization element.
1996,
Pubmed
,
Xenbase
Mowry,
Vegetal messenger RNA localization directed by a 340-nt RNA sequence element in Xenopus oocytes.
1992,
Pubmed
,
Xenbase
Müeller-Pillasch,
Cloning of a gene highly overexpressed in cancer coding for a novel KH-domain containing protein.
1997,
Pubmed
Ross,
Characterization of a beta-actin mRNA zipcode-binding protein.
1997,
Pubmed
Schwartz,
A 69-kDa RNA-binding protein from Xenopus oocytes recognizes a common motif in two vegetally localized maternal mRNAs.
1992,
Pubmed
,
Xenbase
Siomi,
Essential role for KH domains in RNA binding: impaired RNA binding by a mutation in the KH domain of FMR1 that causes fragile X syndrome.
1994,
Pubmed
Siomi,
RNA-binding proteins as regulators of gene expression.
1997,
Pubmed
Stennard,
The Xenopus T-box gene, Antipodean, encodes a vegetally localised maternal mRNA and can trigger mesoderm formation.
1996,
Pubmed
,
Xenbase
St Johnston,
The intracellular localization of messenger RNAs.
1995,
Pubmed
Sundell,
Requirement of microfilaments in sorting of actin messenger RNA.
1991,
Pubmed
Woehlke,
Microtubule interaction site of the kinesin motor.
1997,
Pubmed
Yisraeli,
The material mRNA Vg1 is correctly localized following injection into Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Yisraeli,
A two-step model for the localization of maternal mRNA in Xenopus oocytes: involvement of microtubules and microfilaments in the translocation and anchoring of Vg1 mRNA.
1990,
Pubmed
,
Xenbase
Zhang,
Xenopus VegT RNA is localized to the vegetal cortex during oogenesis and encodes a novel T-box transcription factor involved in mesodermal patterning.
1996,
Pubmed
,
Xenbase
Zhou,
RNA transport to the vegetal cortex of Xenopus oocytes.
1996,
Pubmed
,
Xenbase
Zhou,
Localization of Xcat-2 RNA, a putative germ plasm component, to the mitochondrial cloud in Xenopus stage I oocytes.
1996,
Pubmed
,
Xenbase
