Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
RNA
2006 Jun 01;126:1084-91. doi: 10.1261/rna.59606.
Show Gene links
Show Anatomy links
CUG-BP binds to RNA substrates and recruits PARN deadenylase.
Moraes KC
,
Wilusz CJ
,
Wilusz J
.
???displayArticle.abstract??? CUG-BP is the human homolog of the Xenopus EDEN-BP, which was shown previously to bind to mRNAs, such as c-mos, that exhibit rapid deadenylation following fertilization of the oocyte. While several studies have focused on roles of CUG-BP as a splicing or translation regulator in mammalian cells, its role in mRNA decay has not been examined in detail. Here, we have used an in vitro deadenylation assay to dissect the function of CUG-BP in the decay of two ARE-containing mRNAs: c-fos and TNFalpha. CUG-BP binds specifically to both of these RNAs and stimulates poly(A) shortening by PARN. Moreover, CUG-BP interacts with PARN in extracts by coimmunoprecipitation, and this interaction can be recapitulated using recombinant proteins. CUG-BP, therefore, is the first RNA-binding protein shown to directly recruit a deadenylase to an RNA substrate.
Audic,
Embryo deadenylation element-dependent deadenylation is enhanced by a cis element containing AUU repeats.
1998, Pubmed,
Xenbase
Audic,
Embryo deadenylation element-dependent deadenylation is enhanced by a cis element containing AUU repeats.
1998,
Pubmed
,
Xenbase
Bonnet-Corven,
An analysis of the sequence requirements of EDEN-BP for specific RNA binding.
2002,
Pubmed
,
Xenbase
Detivaud,
Regulation of EDEN-dependent deadenylation of Aurora A/Eg2-derived mRNA via phosphorylation and dephosphorylation in Xenopus laevis egg extracts.
2003,
Pubmed
,
Xenbase
Dignam,
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
1983,
Pubmed
Faustino,
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
2005,
Pubmed
Ford,
ELAV proteins stabilize deadenylated intermediates in a novel in vitro mRNA deadenylation/degradation system.
1999,
Pubmed
Ford,
An in vitro system using HeLa cytoplasmic extracts that reproduces regulated mRNA stability.
1999,
Pubmed
Gao,
Interaction between a poly(A)-specific ribonuclease and the 5' cap influences mRNA deadenylation rates in vitro.
2000,
Pubmed
Gherzi,
A KH domain RNA binding protein, KSRP, promotes ARE-directed mRNA turnover by recruiting the degradation machinery.
2004,
Pubmed
Ho,
Transgenic mice expressing CUG-BP1 reproduce splicing mis-regulation observed in myotonic dystrophy.
2005,
Pubmed
Kahvejian,
Mammalian poly(A)-binding protein is a eukaryotic translation initiation factor, which acts via multiple mechanisms.
2005,
Pubmed
Körner,
The deadenylating nuclease (DAN) is involved in poly(A) tail removal during the meiotic maturation of Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Lai,
Tristetraprolin and its family members can promote the cell-free deadenylation of AU-rich element-containing mRNAs by poly(A) ribonuclease.
2003,
Pubmed
Legagneux,
Substrate-specific regulation of RNA deadenylation in Xenopus embryo and activated egg extracts.
1995,
Pubmed
,
Xenbase
Lu,
Cardiac elav-type RNA-binding protein (ETR-3) binds to RNA CUG repeats expanded in myotonic dystrophy.
1999,
Pubmed
Lykke-Andersen,
Recruitment and activation of mRNA decay enzymes by two ARE-mediated decay activation domains in the proteins TTP and BRF-1.
2005,
Pubmed
Mukherjee,
The mammalian exosome mediates the efficient degradation of mRNAs that contain AU-rich elements.
2002,
Pubmed
Mukhopadhyay,
Coupled mRNA stabilization and translational silencing of cyclooxygenase-2 by a novel RNA binding protein, CUGBP2.
2003,
Pubmed
Paillard,
Poly(A) metabolism in Xenopus laevis embryos: substrate-specific and default poly(A) nuclease activities are mediated by two distinct complexes.
1996,
Pubmed
,
Xenbase
Paillard,
EDEN and EDEN-BP, a cis element and an associated factor that mediate sequence-specific mRNA deadenylation in Xenopus embryos.
1998,
Pubmed
,
Xenbase
Paillard,
c-Jun ARE targets mRNA deadenylation by an EDEN-BP (embryo deadenylation element-binding protein)-dependent pathway.
2002,
Pubmed
,
Xenbase
Paillard,
A functional deadenylation assay identifies human CUG-BP as a deadenylation factor.
2003,
Pubmed
,
Xenbase
Philips,
Disruption of splicing regulated by a CUG-binding protein in myotonic dystrophy.
1998,
Pubmed
Richter,
Cytoplasmic polyadenylation in development and beyond.
1999,
Pubmed
,
Xenbase
Roberts,
Altered phosphorylation and intracellular distribution of a (CUG)n triplet repeat RNA-binding protein in patients with myotonic dystrophy and in myotonin protein kinase knockout mice.
1997,
Pubmed
Takahashi,
The CUG-binding protein binds specifically to UG dinucleotide repeats in a yeast three-hybrid system.
2000,
Pubmed
Timchenko,
RNA CUG-binding protein 1 increases translation of 20-kDa isoform of CCAAT/enhancer-binding protein beta by interacting with the alpha and beta subunits of eukaryotic initiation translation factor 2.
2005,
Pubmed
Timchenko,
Identification of a (CUG)n triplet repeat RNA-binding protein and its expression in myotonic dystrophy.
1996,
Pubmed
Voeltz,
AUUUA sequences direct mRNA deadenylation uncoupled from decay during Xenopus early development.
1998,
Pubmed
,
Xenbase
Wilusz,
Bringing the role of mRNA decay in the control of gene expression into focus.
2004,
Pubmed
Wilusz,
A 64 kd nuclear protein binds to RNA segments that include the AAUAAA polyadenylation motif.
1988,
Pubmed
Xu,
Modulation of the fate of cytoplasmic mRNA by AU-rich elements: key sequence features controlling mRNA deadenylation and decay.
1997,
Pubmed
