Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
TFIIH functions in regulating transcriptional elongation by RNA polymerase II in Xenopus oocytes.
Yankulov KY
,
Pandes M
,
McCracken S
,
Bouchard D
,
Bentley DL
.
???displayArticle.abstract???
We investigated the role of TFIIH in transcription by RNA polymerase II (pol II) in vivo by microinjection of antibodies against this factor into Xenopus oocytes. Five different antibodies directed against four subunits of TFIIH were tested for effects on transcription of coinjected human immunodeficiency virus type 2 and c-myc templates. Each of these antibodies severely reduced the efficiency of elongation through human immunodeficiency virus type 2 and c-myc terminator elements. In contrast, an anti-TFIIB antibody did not inhibit elongation. Anti-TFIIH antibodies also had a much smaller inhibitory effect on total transcription than did anti-TFIIB or anti-pol II large subunit. Three inhibitors of TFIIH kinase activity, H-7, H-8, and dichlororibofuranosylbenzimidazole (DRB), inhibited elongation similarly to anti-TFIIH antibodies. These results strongly suggest a role for TFIIH in the stimulation of transcriptional elongation in vivo.
Bentley,
Sequence requirements for premature termination of transcription in the human c-myc gene.
1988, Pubmed,
Xenbase
Bentley,
Sequence requirements for premature termination of transcription in the human c-myc gene.
1988,
Pubmed
,
Xenbase
Blau,
Three functional classes of transcriptional activation domain.
1996,
Pubmed
Boerrigter,
Plasmid-based transgenic mouse model for studying in vivo mutations.
1995,
Pubmed
Chodosh,
5,6-Dichloro-1-beta-D-ribofuranosylbenzimidazole inhibits transcription elongation by RNA polymerase II in vitro.
1989,
Pubmed
Cismowski,
KIN28 encodes a C-terminal domain kinase that controls mRNA transcription in Saccharomyces cerevisiae but lacks cyclin-dependent kinase-activating kinase (CAK) activity.
1995,
Pubmed
Devault,
MAT1 ('menage à trois') a new RING finger protein subunit stabilizing cyclin H-cdk7 complexes in starfish and Xenopus CAK.
1995,
Pubmed
,
Xenbase
Drapkin,
The multifunctional TFIIH complex and transcriptional control.
1994,
Pubmed
Emerman,
The specificity of the human immunodeficiency virus type 2 transactivator is different from that of human immunodeficiency virus type 1.
1987,
Pubmed
Evan,
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
1985,
Pubmed
,
Xenbase
Feaver,
Relationship of CDK-activating kinase and RNA polymerase II CTD kinase TFIIH/TFIIK.
1994,
Pubmed
Fischer,
Cloning of the 62-kilodalton component of basic transcription factor BTF2.
1992,
Pubmed
Fisher,
Alternative mechanisms of CAK assembly require an assembly factor or an activating kinase.
1995,
Pubmed
Fisher,
A novel cyclin associates with MO15/CDK7 to form the CDK-activating kinase.
1994,
Pubmed
Goodrich,
Transcription factors IIE and IIH and ATP hydrolysis direct promoter clearance by RNA polymerase II.
1994,
Pubmed
Graves,
Homologous recognition of a promoter domain common to the MSV LTR and the HSV tk gene.
1986,
Pubmed
Gurdon,
The use of Xenopus oocytes for the expression of cloned genes.
1983,
Pubmed
,
Xenbase
Guzder,
DNA repair gene RAD3 of S. cerevisiae is essential for transcription by RNA polymerase II.
1994,
Pubmed
Guzder,
RAD25 is a DNA helicase required for DNA repair and RNA polymerase II transcription.
1994,
Pubmed
Ha,
Cloning of a human gene encoding the general transcription initiation factor IIB.
1991,
Pubmed
Harland,
Transcription of DNA injected into Xenopus oocytes is influenced by template topology.
1983,
Pubmed
,
Xenbase
Holstege,
The requirement for the basal transcription factor IIE is determined by the helical stability of promoter DNA.
1995,
Pubmed
Humbert,
p44 and p34 subunits of the BTF2/TFIIH transcription factor have homologies with SSL1, a yeast protein involved in DNA repair.
1994,
Pubmed
Izaurralde,
A cap-binding protein complex mediating U snRNA export.
1995,
Pubmed
,
Xenbase
Izban,
The RNA polymerase II ternary complex cleaves the nascent transcript in a 3'----5' direction in the presence of elongation factor SII.
1992,
Pubmed
Jiang,
Nucleotide requirements for activated RNA polymerase II open complex formation in vitro.
1995,
Pubmed
Kenny,
The role of human single-stranded DNA binding protein and its individual subunits in simian virus 40 DNA replication.
1990,
Pubmed
Krumm,
The block to transcriptional elongation within the human c-myc gene is determined in the promoter-proximal region.
1992,
Pubmed
London,
Analysis of premature termination in c-myc during transcription by RNA polymerase II in a HeLa nuclear extract.
1991,
Pubmed
Mäkelä,
A kinase-deficient transcription factor TFIIH is functional in basal and activated transcription.
1995,
Pubmed
McKnight,
Transcriptional control signals of a eukaryotic protein-coding gene.
1982,
Pubmed
Meulia,
Sequences in the human c-myc P2 promoter affect the elongation and premature termination of transcripts initiated from the upstream P1 promoter.
1992,
Pubmed
,
Xenbase
Meulia,
Distinct properties of c-myc transcriptional elongation are revealed in Xenopus oocytes and mammalian cells and by template titration, 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB), and promoter mutagenesis.
1993,
Pubmed
,
Xenbase
Miller,
A cis-acting element in the promoter region of the murine c-myc gene is necessary for transcriptional block.
1989,
Pubmed
O'Brien,
Phosphorylation of RNA polymerase II C-terminal domain and transcriptional elongation.
1994,
Pubmed
Pan,
Initiation of transcription by RNA polymerase II is limited by melting of the promoter DNA in the region immediately upstream of the initiation site.
1994,
Pubmed
Parvin,
Promoter specificity of basal transcription factors.
1992,
Pubmed
Parvin,
DNA topology and a minimal set of basal factors for transcription by RNA polymerase II.
1993,
Pubmed
Poon,
Cell cycle regulation of the p34cdc2/p33cdk2-activating kinase p40MO15.
1994,
Pubmed
,
Xenbase
Price,
Dynamic interaction between a Drosophila transcription factor and RNA polymerase II.
1989,
Pubmed
Qiu,
The Saccharomyces cerevisiae DNA repair gene RAD25 is required for transcription by RNA polymerase II.
1993,
Pubmed
Roberts,
Distinct modes of transcription read through or terminate at the c-myc attenuator.
1992,
Pubmed
,
Xenbase
Roy,
The MO15 cell cycle kinase is associated with the TFIIH transcription-DNA repair factor.
1994,
Pubmed
Schaeffer,
DNA repair helicase: a component of BTF2 (TFIIH) basic transcription factor.
1993,
Pubmed
Schaeffer,
The ERCC2/DNA repair protein is associated with the class II BTF2/TFIIH transcription factor.
1994,
Pubmed
Serizawa,
Multifunctional RNA polymerase II initiation factor delta from rat liver. Relationship between carboxyl-terminal domain kinase, ATPase, and DNA helicase activities.
1993,
Pubmed
Serizawa,
Association of Cdk-activating kinase subunits with transcription factor TFIIH.
1995,
Pubmed
Seroz,
TFIIH: a link between transcription, DNA repair and cell cycle regulation.
1995,
Pubmed
Shiekhattar,
Cdk-activating kinase complex is a component of human transcription factor TFIIH.
1995,
Pubmed
Strobl,
Hold back of RNA polymerase II at the transcription start site mediates down-regulation of c-myc in vivo.
1992,
Pubmed
Tamm,
Definition of subclasses of nucleoplasmic RNA.
1977,
Pubmed
Thompson,
Purification of eukaryotic RNA polymerase II by immunoaffinity chromatography. Elution of active enzyme with protein stabilizing agents from a polyol-responsive monoclonal antibody.
1990,
Pubmed
Timmers,
Transcription initiation by RNA polymerase II does not require hydrolysis of the beta-gamma phosphoanhydride bond of ATP.
1994,
Pubmed
Toohey,
In vitro formation of short RNA polymerase II transcripts that terminate within the HIV-1 and HIV-2 promoter-proximal downstream regions.
1989,
Pubmed
Valay,
The KIN28 gene is required both for RNA polymerase II mediated transcription and phosphorylation of the Rpb1p CTD.
1995,
Pubmed
Wang,
Transcription factor b (TFIIH) is required during nucleotide-excision repair in yeast.
1994,
Pubmed
Xiao,
Binding of basal transcription factor TFIIH to the acidic activation domains of VP16 and p53.
1994,
Pubmed
Yankulov,
The transcriptional elongation inhibitor 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole inhibits transcription factor IIH-associated protein kinase.
1995,
Pubmed
Yankulov,
Transcriptional elongation by RNA polymerase II is stimulated by transactivators.
1994,
Pubmed
,
Xenbase
Zawel,
Recycling of the general transcription factors during RNA polymerase II transcription.
1995,
Pubmed
