Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
Na+ currents recorded from Xenopus oocytes expressing the Na+ channel alpha subunit alone inactivate with two exponential components. The slow component predominates in monomeric channels, while co-expression with the beta 1 subunit favors the fast component. Macropatch recordings show that the relative rates of these components are much greater than previously estimated from two-electrode measurements (approximately 30-fold vs approximately 5-fold). A re-assessment of steady-state inactivation, h infinity (V), shows that there is no depolarized shift of the slow component, provided a sufficiently long prepulse duration and repetition interval are used to achieve steady-state entry and recovery from inactivation, respectively. Deletion mutagenesis of the beta 1 subunit was used to define which regions of the subunit are required to modulate inactivation kinetics. The carboxy tail, comprising the entire predicted intracellular domain, can be deleted without a loss of activity; whereas small deletions in the extracellular amino domain or the signal peptide totally disrupt function.
Bennett,
A molecular basis for gating mode transitions in human skeletal muscle Na+ channels.
1993, Pubmed,
Xenbase
Bennett,
A molecular basis for gating mode transitions in human skeletal muscle Na+ channels.
1993,
Pubmed
,
Xenbase
Cannon,
Modification of the Na+ current conducted by the rat skeletal muscle alpha subunit by coexpression with a human brain beta subunit.
1993,
Pubmed
Cannon,
Theoretical reconstruction of myotonia and paralysis caused by incomplete inactivation of sodium channels.
1993,
Pubmed
Cannon,
A sodium channel defect in hyperkalemic periodic paralysis: potassium-induced failure of inactivation.
1991,
Pubmed
Catterall,
Cellular and molecular biology of voltage-gated sodium channels.
1992,
Pubmed
Fernandez,
Membrane patches and whole-cell membranes: a comparison of electrical properties in rat clonal pituitary (GH3) cells.
1984,
Pubmed
Fleig,
Kinetic mode switch of rat brain IIA Na channels in Xenopus oocytes excised macropatches.
1994,
Pubmed
,
Xenbase
Ho,
Site-directed mutagenesis by overlap extension using the polymerase chain reaction.
1989,
Pubmed
Isom,
Primary structure and functional expression of the beta 1 subunit of the rat brain sodium channel.
1992,
Pubmed
,
Xenbase
Isom,
Auxiliary subunits of voltage-gated ion channels.
1994,
Pubmed
Ji,
Voltage-dependent regulation of modal gating in the rat SkM1 sodium channel expressed in Xenopus oocytes.
1994,
Pubmed
,
Xenbase
Krafte,
Evidence for the involvement of more than one mRNA species in controlling the inactivation process of rat and rabbit brain Na channels expressed in Xenopus oocytes.
1988,
Pubmed
,
Xenbase
Krafte,
Inactivation of cloned Na channels expressed in Xenopus oocytes.
1990,
Pubmed
,
Xenbase
Kupper,
Intracellular and extracellular amino acids that influence C-type inactivation and its modulation in a voltage-dependent potassium channel.
1995,
Pubmed
,
Xenbase
Makita,
Voltage-gated Na+ channel beta 1 subunit mRNA expressed in adult human skeletal muscle, heart, and brain is encoded by a single gene.
1994,
Pubmed
,
Xenbase
McClatchey,
The cloning and expression of a sodium channel beta 1-subunit cDNA from human brain.
1993,
Pubmed
,
Xenbase
Moorman,
Fast and slow gating of sodium channels encoded by a single mRNA.
1990,
Pubmed
,
Xenbase
Patton,
The adult rat brain beta 1 subunit modifies activation and inactivation gating of multiple sodium channel alpha subunits.
1994,
Pubmed
,
Xenbase
Scheuer,
Functional properties of rat brain sodium channels expressed in a somatic cell line.
1990,
Pubmed
Simoncini,
Slow sodium channel inactivation in rat fast-twitch muscle.
1987,
Pubmed
Stühmer,
Patch clamp characterization of sodium channels expressed from rat brain cDNA.
1987,
Pubmed
,
Xenbase
Stühmer,
Structural parts involved in activation and inactivation of the sodium channel.
1989,
Pubmed
,
Xenbase
Tejedor,
Site of covalent attachment of alpha-scorpion toxin derivatives in domain I of the sodium channel alpha subunit.
1988,
Pubmed
Trimmer,
Primary structure and functional expression of a mammalian skeletal muscle sodium channel.
1989,
Pubmed
,
Xenbase
Ukomadu,
muI Na+ channels expressed transiently in human embryonic kidney cells: biochemical and biophysical properties.
1992,
Pubmed
Zhou,
Multiple gating modes and the effect of modulating factors on the microI sodium channel.
1991,
Pubmed
,
Xenbase
