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Purification of a DNA-binding protein from Xenopus laevis unfertilized eggs.
Carrara G
,
Gattoni S
,
Mercanti D
,
Tocchini-Valentini GP
.
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A DNA-binding protein from Xenopus laevis unfertilized eggs has been purified to apparent homogeneity. It is a heat stable, lysine-rich protein and has a molecular weight corresponding to 8,200 daltons, measured by sodium dodecyl sulphate gel electrophoresis. The protein, which is active in a monomeric form, stimulates DNA polymerase alpha, and binds to single and double stranded DNA. One egg contains about 4 x 10(12) molecules (minimum estimate) of the protein; since we calculate that 4 x 10(8) molecules are sufficient to cover the entire genome (haploid complement), there is much more protein than is needed to cover chromosomal DNA.
Adamson,
Histone synthesis in early amphibian development: histone and DNA syntheses are not co-ordinated.
1974, Pubmed,
Xenbase
Adamson,
Histone synthesis in early amphibian development: histone and DNA syntheses are not co-ordinated.
1974,
Pubmed
,
Xenbase
Alberts,
Isolation and characterization of gene 5 protein of filamentous bacterial viruses.
1972,
Pubmed
Baldi,
Particulate DNA polymerase from the cytoplasm of Xenopus laevis oocytes.
1976,
Pubmed
,
Xenbase
Banks,
The isolation and properties of a DNA-unwinding protein from Ustilago maydis.
1975,
Pubmed
Brown,
Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.
1968,
Pubmed
Callan,
Replication of DNA in eukaryotic chromosomes.
1973,
Pubmed
Dawid,
Deoxyribonucleic acid in amphibian eggs.
1965,
Pubmed
Gandini Attardi,
Effect of Xenopus laevis oocyte extract on supercoiled simian virus 40 DNA: formation of complex DNA.
1976,
Pubmed
,
Xenbase
Gold,
Regulation of gene 32 expression during bacteriophage T4 infection of Escherichia coli.
1976,
Pubmed
Gurdon,
The appearance of cytoplasmic DNA polymerase activity during the maturation of amphibian oocytes into eggs.
1969,
Pubmed
Herrick,
Single-stranded DNA structure and DNA polymerase activity in the presence of nucleic acid helix-unwinding proteins from calf thymus.
1976,
Pubmed
Herrick,
Nucleic acid helix-coil transitions mediated by helix-unwinding proteins from calf thymus.
1976,
Pubmed
Hotta,
A DNA-binding protein in meiotic cells of Lilium.
1971,
Pubmed
Kedes,
Histone messengers and histone genes.
1976,
Pubmed
Keller,
Characterization of purified DNA-relaxing enzyme from human tissue culture cells.
1975,
Pubmed
Litman,
A deoxyribonucleic acid polymerase from Micrococcus luteus (Micrococcus lysodeikticus) isolated on deoxyribonucleic acid-cellulose.
1968,
Pubmed
LOWRY,
Protein measurement with the Folin phenol reagent.
1951,
Pubmed
Martini,
Nuclear localization of DNA polymerase alpha in Xenopus laevis oocytes.
1976,
Pubmed
,
Xenbase
Mattoccia,
DNA-relaxing activity and endonuclease activity in Xenopus laevis oocytes.
1976,
Pubmed
,
Xenbase
Oey,
Properties of the isolated gene 5 protein of bacteriophage fd.
1972,
Pubmed
Perkowska,
Gene amplification in the oocyte nucleus of mutant and wild-type Xenopus laevis.
1968,
Pubmed
,
Xenbase
Reuben,
A DNA-binding protein induced by bacteriophage T7.
1973,
Pubmed
Reuben,
A deoxyribonucleic acid-binding protein induced by bacteriophage T7. Purification and properties of the protein.
1974,
Pubmed
Scherzinger,
Stimulation of T7 DNA polymerase by a new phage-coded protein.
1973,
Pubmed
Sigal,
A DNA-unwinding protein isolated from Escherichia coli: its interaction with DNA and with DNA polymerases.
1972,
Pubmed
Spadari,
HeLa cell R-deoxyribonucleic acid polymerases. Separation and characterization of two enzymatic activities.
1974,
Pubmed
Tatò,
Major DNA polymerases common to different Xenopus laevis cell types.
1974,
Pubmed
,
Xenbase
Weber,
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
1969,
Pubmed
Weissbach,
Vertebrate DNA polymerases.
1975,
Pubmed
