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The dorsal-specific homeobox gene goosecoid (gsc) and the bone morphogenetic protein 4 gene (BMP-4) are expressed in complementary regions of the Xenopus gastrula. Injection of gsc mRNA dorsalizes ventral mesodermal tissue and can induce axis formation in normal and UV-ventralized embryos. On the other hand, BMP-4 mRNA injection, which has a strong ventralizing effect on whole embryos, has been implicated in ventralization by UV, and can rescue tail structures in embryos dorsalized by LiCl. The above-mentioned putative roles for BMP-4 and gsc are based on gain-of-function experiments. In order to determine the in vivo role of these two genes in the patterning of the Xenopus mesoderm during gastrulation, partial loss-of-function experiments were performed using antisense RNA injections. Using marker genes that are expressed early in gastrulation, we show that antisense gsc RNA has a ventralizing effect on embryos, whereas antisense BMP-4 RNA dorsalizes mesodermal tissue. These loss-of-function studies also show a requirement for gsc and BMP-4 in the dorsalization induced by LiCl and in the ventralization generated by UV irradiation, respectively. Thus, both gain- and loss-of-function results for gsc and BMP-4 support the view that these two genes are necessary components of the dorsal and ventral patterning pathways in Xenopus embryos.
Bass,
A developmentally regulated activity that unwinds RNA duplexes.
1987, Pubmed,
Xenbase
Bass,
A developmentally regulated activity that unwinds RNA duplexes.
1987,
Pubmed
,
Xenbase
Blumberg,
Organizer-specific homeobox genes in Xenopus laevis embryos.
1991,
Pubmed
,
Xenbase
Cho,
Molecular nature of Spemann's organizer: the role of the Xenopus homeobox gene goosecoid.
1991,
Pubmed
,
Xenbase
Cho,
Differential utilization of the same reading frame in a Xenopus homeobox gene encodes two related proteins sharing the same DNA-binding specificity.
1988,
Pubmed
,
Xenbase
Chomczynski,
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
1987,
Pubmed
Christian,
Xwnt-8, a Xenopus Wnt-1/int-1-related gene responsive to mesoderm-inducing growth factors, may play a role in ventral mesodermal patterning during embryogenesis.
1991,
Pubmed
,
Xenbase
Christian,
Interactions between Xwnt-8 and Spemann organizer signaling pathways generate dorsoventral pattern in the embryonic mesoderm of Xenopus.
1993,
Pubmed
,
Xenbase
Dale,
Bone morphogenetic protein 4: a ventralizing factor in early Xenopus development.
1992,
Pubmed
,
Xenbase
Dent,
A whole-mount immunocytochemical analysis of the expression of the intermediate filament protein vimentin in Xenopus.
1989,
Pubmed
,
Xenbase
Fainsod,
On the function of BMP-4 in patterning the marginal zone of the Xenopus embryo.
1994,
Pubmed
,
Xenbase
Gerhart,
Cortical rotation of the Xenopus egg: consequences for the anteroposterior pattern of embryonic dorsal development.
1989,
Pubmed
,
Xenbase
Giebelhaus,
Antisense RNA inhibits expression of membrane skeleton protein 4.1 during embryonic development of Xenopus.
1988,
Pubmed
,
Xenbase
Graff,
Studies with a Xenopus BMP receptor suggest that ventral mesoderm-inducing signals override dorsal signals in vivo.
1994,
Pubmed
,
Xenbase
Grunz,
The four animal blastomeres of the eight-cell stage of Xenopus laevis are intrinsically capable of differentiating into dorsal mesodermal derivatives.
1994,
Pubmed
,
Xenbase
Harland,
Stability of RNA in developing Xenopus embryos and identification of a destabilizing sequence in TFIIIA messenger RNA.
1988,
Pubmed
,
Xenbase
Harland,
The transforming growth factor beta family and induction of the vertebrate mesoderm: bone morphogenetic proteins are ventral inducers.
1994,
Pubmed
,
Xenbase
Harland,
In situ hybridization: an improved whole-mount method for Xenopus embryos.
1991,
Pubmed
,
Xenbase
Heasman,
Overexpression of cadherins and underexpression of beta-catenin inhibit dorsal mesoderm induction in early Xenopus embryos.
1994,
Pubmed
,
Xenbase
Hemmati-Brivanlou,
Follistatin, an antagonist of activin, is expressed in the Spemann organizer and displays direct neuralizing activity.
1994,
Pubmed
,
Xenbase
Hogan,
Growth factors in development: the role of TGF-beta related polypeptide signalling molecules in embryogenesis.
1994,
Pubmed
,
Xenbase
Jones,
DVR-4 (bone morphogenetic protein-4) as a posterior-ventralizing factor in Xenopus mesoderm induction.
1992,
Pubmed
,
Xenbase
Kao,
The entire mesodermal mantle behaves as Spemann's organizer in dorsoanterior enhanced Xenopus laevis embryos.
1988,
Pubmed
,
Xenbase
Kessler,
Vertebrate embryonic induction: mesodermal and neural patterning.
1994,
Pubmed
,
Xenbase
Kim,
Double-stranded RNA adenosine deaminase as a potential mammalian RNA editing factor.
1993,
Pubmed
Kimelman,
An antisense mRNA directs the covalent modification of the transcript encoding fibroblast growth factor in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Kintner,
Monoclonal antibodies identify blastemal cells derived from dedifferentiating limb regeneration.
,
Pubmed
,
Xenbase
Köster,
Bone morphogenetic protein 4 (BMP-4), a member of the TGF-beta family, in early embryos of Xenopus laevis: analysis of mesoderm inducing activity.
1991,
Pubmed
,
Xenbase
Kyhse-Andersen,
Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose.
1984,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lemaire,
Expression cloning of Siamois, a Xenopus homeobox gene expressed in dorsal-vegetal cells of blastulae and able to induce a complete secondary axis.
1995,
Pubmed
,
Xenbase
Maéno,
A truncated bone morphogenetic protein 4 receptor alters the fate of ventral mesoderm to dorsal mesoderm: roles of animal pole tissue in the development of ventral mesoderm.
1994,
Pubmed
,
Xenbase
Niehrs,
Mesodermal patterning by a gradient of the vertebrate homeobox gene goosecoid.
1994,
Pubmed
,
Xenbase
Niehrs,
The homeobox gene goosecoid controls cell migration in Xenopus embryos.
1993,
Pubmed
,
Xenbase
Nishimatsu,
Genes for bone morphogenetic proteins are differentially transcribed in early amphibian embryos.
1992,
Pubmed
,
Xenbase
Rebagliati,
Antisense RNA injections in fertilized frog eggs reveal an RNA duplex unwinding activity.
1987,
Pubmed
,
Xenbase
Rivera-Pérez,
Goosecoid is not an essential component of the mouse gastrula organizer but is required for craniofacial and rib development.
1995,
Pubmed
,
Xenbase
Sanes,
Use of a recombinant retrovirus to study post-implantation cell lineage in mouse embryos.
1986,
Pubmed
Sasai,
Xenopus chordin: a novel dorsalizing factor activated by organizer-specific homeobox genes.
1994,
Pubmed
,
Xenbase
Sasai,
Two mammalian helix-loop-helix factors structurally related to Drosophila hairy and Enhancer of split.
1992,
Pubmed
Sasai,
Regulation of neural induction by the Chd and Bmp-4 antagonistic patterning signals in Xenopus.
1995,
Pubmed
,
Xenbase
Schmidt,
Localized BMP-4 mediates dorsal/ventral patterning in the early Xenopus embryo.
1995,
Pubmed
,
Xenbase
Schulte-Merker,
Effects of truncated activin and FGF receptors and of follistatin on the inducing activities of BVg1 and activin: does activin play a role in mesoderm induction?
1994,
Pubmed
,
Xenbase
Smith,
Expression cloning of noggin, a new dorsalizing factor localized to the Spemann organizer in Xenopus embryos.
1992,
Pubmed
,
Xenbase
Smith,
Biochemical specificity of Xenopus notochord.
1985,
Pubmed
,
Xenbase
Smith,
Injected Xwnt-8 RNA acts early in Xenopus embryos to promote formation of a vegetal dorsalizing center.
1991,
Pubmed
,
Xenbase
Steinbeisser,
Xenopus axis formation: induction of goosecoid by injected Xwnt-8 and activin mRNAs.
1993,
Pubmed
,
Xenbase
Suzuki,
A truncated bone morphogenetic protein receptor affects dorsal-ventral patterning in the early Xenopus embryo.
1994,
Pubmed
,
Xenbase
Taira,
Role of the LIM class homeodomain protein Xlim-1 in neural and muscle induction by the Spemann organizer in Xenopus.
1994,
Pubmed
,
Xenbase
von Dassow,
Induction of the Xenopus organizer: expression and regulation of Xnot, a novel FGF and activin-regulated homeo box gene.
1993,
Pubmed
,
Xenbase
Yamada,
Targeted mutation of the murine goosecoid gene results in craniofacial defects and neonatal death.
1995,
Pubmed
